Plastic filling material used for surgical treatment of bone defect and preparation method thereof

Abstract

The invention provides a plastic filling material used for surgical treatment of a bone defect and a preparation method thereof. The method comprises the steps of using an allogenous cortical bone forpreparing bone grains in particle size of 0.1-1 mm; adopting a chloroform / methyl alcohol (1 to 1) solution for room-temperature soak degreasing; in purified water at 60+ / -1 DEG C, soaking the bone grains subjected to degreasing for 10 h to conduct virus inactivation; in hydrochloric acid or acetic acid, using an ultrasonic method for conducting demineralization treatment, wherein the residual calcium amount of the bone grains subjected to demineralization treatment is lower than 8%; using purified water for ultrasonic cleaning, and replacing the water hourly until a cleaning liquid pH is 5.8-7.5; conducting freeze drying treatment, using two layers of bacteria-isolating plastic bags for packaging, and making the bone grains subjected to irradiation sterilization for use; and in a sterileenvironment, preparing a hyaluronic acid solution in a concentration of 2.5%-2.9%, and adding 450 mg of the bone grains into each milliliter of hyaluronic acid solution for mixing. By means of the provided material and method, without changing bone induction activity of the demineralized bone, the plastic mixture of the demineralized bone matrix and hyaluronic acid can be prepared, and can be directly used in clinic.

Description

technical field [0001] The invention belongs to the technical field of filling materials for surgical treatment of bone defects, in particular to a plastic filling material for surgical treatment of bone defects and a preparation method thereof. Background technique [0002] At present, allogeneic bone implant materials are widely used as bone graft materials for bone defect repair and reconstruction at home and abroad. Due to the natural osteoconductivity and osteoinductive activity of this material, it has a good clinical application as a filling of general bone defects, spinal fusion, filling of oral and maxillofacial bone defects, and bone grafting and structural reconstruction of bone defects caused by other reasons. treatment effect. Demineralized bone matrix is ​​a homogeneous bone material that has been treated with appropriate acidity, virus inactivation, and freeze-drying. It retains and exposes natural bone morphogenetic proteins, thereby increasing its osteoindu...

AI Technical Summary

Problems solved by technology

Demineralized bone matrix is ​​currently widely used abroad. However, when filling bone defects with the same prefabricated bone repair material in clinical orthopedics, stomatology and plastic surgery, the demineralized bone matrix has a high strength and is not easy to shape. There is often a problem that the implant material does not match the shape of the irregular bone defect, and there will be incomplete filling and dead space left, resulting in delayed union or nonunion of the bone

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