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A kit for the determination of serum amyloid a content by nephelometry

A technology of serum amyloid and scattering turbidimetry, which is applied in the field of biotechnology and medical inspection, can solve problems such as inaccurate detection results, difficulty in measuring serum amyloid A concentration, and limited promotion, so as to improve sensitivity, solve detection sensitivity, and improve Effects of Specificity and Accuracy

Active Publication Date: 2021-12-14
山东艾科达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of this method is affected by cholesterol, resulting in inaccurate detection results, which brings difficulties to the determination of serum amyloid A concentration, thus limiting the clinical promotion of such reagents

Method used

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  • A kit for the determination of serum amyloid a content by nephelometry
  • A kit for the determination of serum amyloid a content by nephelometry
  • A kit for the determination of serum amyloid a content by nephelometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 A method for measuring serum amyloid A in serum, plasma or whole blood using a kit

[0024] 1) Preparation of calibrator: Prepare serum amyloid A calibrator antigen standard with different concentration gradients with calibrator diluent, and obtain final concentrations of 0mg / L, 6.3mg / L, 12.6mg / L, 25.2mg / L , 50.3mg / L, 100.6mg / L serum amyloid A calibrator antigen standard solution.

[0025] 2) Venous blood collection, whole blood samples are placed in EDTA or heparin anticoagulant tubes; serum samples are placed in non-anticoagulant tubes; plasma samples are placed in EDTA or heparin anticoagulant tubes, and mixed gently by inversion. Serum and plasma samples were centrifuged at 2500-3000 rpm for 8-10 minutes, and the supernatant liquid (serum or plasma) was collected to obtain samples.

[0026] 3) Use Shandong Aikeda automatic specific protein analyzer to measure, set the instrument parameters as detection temperature 37°C, detection wavelength 650nm, reactio...

Embodiment 2

[0028] Embodiment 2 contains the serum amyloid A assay kit of different alfalfa saponin amounts

[0029] 1. Methods and Reagents

[0030] 1. Serum Amyloid A Determination Kit Containing Different Alfalfa Saponin Amount Assay Reagent 1

[0031] Serum amyloid A assay kit Assay reagent 1-A: 0.2 mol / L phosphate buffer, 0.5 mol / L sodium chloride, 0.02% wt ProClin300, 0% wt alfalfa saponin.

[0032] Serum amyloid A assay reagent 1-B, C, D, E, F, G, H, and I have the same components as reagent 1-A, except that the content of alfalfa saponin is different, which are 0.01%wt alfalfa saponin, 0.02% wt alfalfa saponins, 0.03% wt alfalfa saponins, 0.04% wt alfalfa saponins, 0.05% wt alfalfa saponins, 0.06% wt alfalfa saponins, 0.07% wt alfalfa saponins and 0.08% wt alfalfa saponins.

[0033] 2. Serum amyloid A assay kit assay reagent 2: 0.2mol / L phosphate buffer, 5% (w / v) anti-human serum amyloid A antibody latex, 0.5mol / L sodium chloride, 1% wt bovine serum albumin, 0.02% wt ProClin300...

Embodiment 3

[0042] Example 3 Test in the serum amyloid A assay kit containing different glycerol concentrations

[0043] 1. Methods and Reagents

[0044] 1. Serum Amyloid A Assay Kit Containing Different Glycerol Concentration Assay Reagent 1

[0045] Serum amyloid A assay kit Assay reagent 1: 0.2 mol / L phosphate buffer, 0.5 mol / L sodium chloride, 0.02% wt ProClin300.

[0046] 2. Serum amyloid A assay kit containing different concentrations of glycerol Assay reagent 2-A: 0.2mol / L phosphate buffer, 5% (w / v) anti-human serum amyloid A antibody latex, 0.5mol / L L sodium chloride, 1% wt bovine serum albumin, 0.02% wt ProClin 300; glycerol 0 mmol / L.

[0047] The components in serum amyloid A assay reagents 2-B, C, D, E, F and G are the same as those in reagent 2-A, except that the concentration of glycerol is 1mmol / L, 2mmol / L, 4mmol / L respectively , 6mmol / L, 8mmol / L and 10mmol / L.

[0048] 3. Preparation of serum samples containing different cholesterol contents: Add different amounts of cho...

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Abstract

The invention discloses a kit for measuring serum amyloid A content by scattering turbidimetry. The composition of the kit is simple, adding alfalfa saponin can combine with cholesterol to reduce total cholesterol in serum, and can effectively eliminate the interference of cholesterol. The kit is carried out by nephelometry, and the linear range, correlation and sensitivity of the detection are higher than the existing kits, which significantly improves the sensitivity of serum amyloid A detection and solves the problem of existing serum amyloid A detection reagents. Box detection sensitivity, low precision, and narrow linear range.

Description

technical field [0001] The invention relates to a kit for measuring serum amyloid A content by scattering turbidimetry, which belongs to the field of biotechnology and medical inspection. Background technique [0002] Serum amyloid A (serum amyloid A protein, SAA) is a polymorphic protein family encoded by multiple genes, which belongs to the acute phase response protein and a heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12,000. During inflammation, infectious and non-infectious diseases, its concentration in the blood can rise sharply within hours, up to 1000 times the initial concentration, but the half-life is very short, only about 50 minutes; SAA and HDL Related to its ability to regulate HDL metabolism during inflammation, a particularly important property of SAA is that its degradation products can be deposited in different organs in the form of amyloid A fibrils, which is a serious disease in chronic inflammatory disea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/51
CPCG01N21/51
Inventor 谭柏清齐笑马万山王醒赵志敏
Owner 山东艾科达生物科技有限公司
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