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Preparation and application of a dendritic mesoporous silica/iridium dioxide composite nanometer enzyme

A technology of mesoporous silica and iridium dioxide, applied in organic compound/hydride/coordination complex catalysts, chemical instruments and methods, chemical/physical processes, etc. Problems such as poor particle stability and increased economic costs can achieve the effect of improving thermal stability, considerable application prospects, and good catalytic thermal stability

Active Publication Date: 2019-08-30
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] A large number of studies have found that ultra-small iridium dioxide nanoparticles have ultra-high peroxidase activity, but such ultra-small nanoparticles have poor stability and are difficult to recycle, which greatly increases the economic cost of applications. It is urgent to introduce related carriers to nano Enzyme recycling, dendritic mesoporous silica supports enable stabilization and recycling of ultrasmall iridium dioxide nanoparticles while maintaining their high peroxidase activity

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  • Preparation and application of a dendritic mesoporous silica/iridium dioxide composite nanometer enzyme
  • Preparation and application of a dendritic mesoporous silica/iridium dioxide composite nanometer enzyme
  • Preparation and application of a dendritic mesoporous silica/iridium dioxide composite nanometer enzyme

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Embodiment 1

[0042] The embodiment of the present invention provides a preparation method of dendritic mesoporous silica / iridium dioxide composite nanozyme, comprising the following steps:

[0043] (1) Place an iridium metal target with a purity of 99.99wt% on the bottom of the glass tube, add 3 mL of redistilled ethanol, and then focus a pulsed laser with an energy of 85 mJ, a wavelength of 532 nm, and a focal length of 77 nm on the glass tube immersed in redistilled ethanol The iridium metal target was ablated for 20 minutes to obtain an ethanol dispersion of ultra-small iridium dioxide;

[0044] (2) Dissolve 380 mg of CTAB and 168 mg of sodium salicylate in 25 mL of deionized water containing 95 μL of triethylamine and stir for 1 h, add TEOS to the above mixing system, stir for 1.5 h, and then add 25 mL of absolute ethanol to terminate React, centrifuge for 10 minutes to obtain the product, and wash with ethanol and water 3 times and methanol / hydrochloric acid mixed solution reflux 16h ...

Embodiment 2

[0048] Mix 10 μL of 50 mM TMB solution and 10 μL of 250 mM HO 2 o 2 The solution was added to 970 μL acetic acid-sodium acetate buffer solution, and incubated at 25°C for 5 minutes, and then, a dendritic mesoporous silica / iridium dioxide composite nanozyme dispersion containing 236.2 μg / mL iridium dioxide was added thereto To start the reaction, add only the dendritic mesoporous silica carrier and the substrates TMB and H 2 o 2 , substrate TMB and H 2 o 2 With the substrate TMB as a control, its absorption spectrum was measured in the range of 300-900 nm after reacting at 25° C. for 10 minutes.

[0049] image 3 It is a color photograph and a comparison chart of visible absorption spectrum of dendritic mesoporous silica / iridium dioxide composite nanozyme catalyzed TMB oxidation and control experiment, from image 3 It can be seen that the dendritic mesoporous silica support alone cannot catalyze the color development of TMB, and TMB itself is difficult to be detected by ...

Embodiment 3

[0051] 10 μL 50 mM OPD, ABTS, DAB, DOPA solution and 10 μL 250 mM H 2 o 2 The solution was added to 970 μL acetic acid-sodium acetate buffer solution, and incubated at 25° C. for 5 min, and then, a dendritic mesoporous silica / iridium dioxide composite nanozyme dispersion containing 236.2 μg / mL iridium dioxide was added thereto to To start the reaction, take only 10 μL of 50 mMOPD, ABTS, DAB and DOPA solution in 990 μL of acetic acid-sodium acetate buffer solution as a control, react at 25 °C for 10 min, and measure the absorption spectra in different wavelength ranges.

[0052] Figure 4-7 The color rendering diagram and visible absorption spectrum comparison diagram of the dendritic mesoporous silica / iridium dioxide composite nanozyme catalyzed oxidation of OPD, ABTS, DAB and DOPA and the blank test respectively; it can be seen from the figure that the dendritic mesoporous Silica / iridium dioxide composite nanozyme with catalytic H 2 o 2 Oxidation of OPD showed yellow, oxi...

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Abstract

The invention discloses a preparation and application of a dendritic mesoporous silica / iridium dioxide composite nanometer enzyme. The preparation method comprises the following steps: uniformly mixing iridium dioxide nanoparticles with dendritic mesoporous silica modified by amino group on the surface according to a certain proportion, and standing for 6-12hours. The iridium dioxide nanoparticlesprepared by the laser ablation method have no ligands on the surface, can fully contact with the substrate in the catalysis, and are loaded in the dendritic mesoporous silica to improve the thermal stability of the iridium dioxide nanoparticles, and the iridium dioxide nanoparticles have the ability to be recycled, and have little influence on the catalytic activity of the iridium dioxide nanoparticles. It can catalyze the oxidation colouring of TMB in the presence of H2O2, and can be used to synthesize dendritic mesoporous silica / iridium dioxide composite nanoenzyme-acetylcholinesterase cascade reaction system, so as to evaluate the activity of acetylcholinesterase by detecting the absorbance. It has a considerable application prospect in biochemical fields such as the detection of acetylcholinesterase activity.

Description

technical field [0001] The invention relates to the application field of colorimetric detection of enzyme activity of inorganic composite nanomaterials, in particular to the preparation and application of a dendritic mesoporous silica / iridium dioxide composite nanozyme. Background technique [0002] Inorganic nanozymes are a kind of inorganic nanomaterials with pseudo-natural enzyme catalytic ability. Therefore, compared with the chemical nature of proteins or nucleic acids of biological enzymes, this material has high thermal and chemical stability, and is economical and convenient to prepare and purify. Improving the application of biological enzymes in the field of biochemical research has extraordinary potential. Among inorganic nanozymes, peroxide nanozymes are a class of inorganic nanomaterials with catalytic ability similar to natural peroxidases, and their main function is in H 2 o 2 capable of catalyzing the catalase substrate in the presence of In 2007, the rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/26G01N21/31
CPCB01J31/26G01N21/31
Inventor 杨蓉肖蔚刘学良伏钊蔡双飞王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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