Silicon nanowire chip and mass spectrum detection method based on silicon nanowire chip

Abstract

The invention discloses a silicon nanowire chip and a mass spectrum detection method and detection method based on the silicon nanowire chip. The mass spectrum detection method comprises the followingsteps: step 1, manufacturing the silicon nanowire chip; carrying out surface washing pretreatment on the monocrystalline silicon wafer, carrying out metal-assisted etching, and carrying out alkali etching to obtain a silicon nanowire chip with a tip; carrying out surface chemical or nano material modification on the silicon nanowire chip; step 2, evaluating the mass spectrum performance of the silicon nanowire chip; step 3, carrying out top contact sampling and in-situ ionization mass spectrometry detection. According to the method, the nanostructure characteristic and the semiconductor characteristic of the silicon nanowire chip are fully utilized, contact type extraction transfer printing and matrix-free mass spectrometry detection are integrated, and the collection, pretreatment and detection processes of complex samples are greatly simplified; the silicon nanowire chip manufactured by the method has adsorption and extraction functions and a mass spectrum detection function at thesame time, and in-situ information of a sample with spatial heterogeneity can be reserved.

Description

technical field [0001] The invention belongs to the technical field of new materials and mass spectrometry detection and analysis methods, and in particular relates to a silicon nanowire chip and a mass spectrometry detection method based on the silicon nanowire chip. Background technique [0002] The collection, pretreatment and detection of biological samples are three important links in the field of analytical technology. Compared with conventional analysis methods, mass spectrometry has the characteristics of high accuracy, high sensitivity, and high content, and is especially suitable for qualitative and quantitative analysis of trace analytes in complex samples and parallel analysis of multiple analytes. play an important role in clinical diagnosis. Among them, the method based on matrix-assisted laser desorption / ionization (MALDI) provides a good platform for the rapid detection of complex samples due to its high speed, high throughput, and strong anti-pollution char...

AI Technical Summary

Problems solved by technology

Although a variety of nanomaterials can solve the problem of background interference, their role is limited to replacing organic matrices, and the sample collection and pretreatment processes required in traditional MALDI are still indispensable

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