Streptomycete TJ561, product for preventing and controlling successive cropping obstacle of soil, preparation method and application thereof and application of eugenol

A soil continuous cropping obstacle, TJ561 technology, applied in the field of microorganisms, can solve the problems of low utilization rate of Streptomyces, low rate of Streptomyces fermentation sporulation, etc., to improve soil continuous cropping obstacles, improve vegetable disease resistance, and suppress diseases.

Active Publication Date: 2019-09-10
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Description
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  • Application Information

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Problems solved by technology

[0009] The third object of the present invention is to provide a method for improving the sporulation rate of Streptomyces TJ561, so as to alleviate the technical problems of low Streptomyces fermentation sporulation rate and low utilization rate of Streptomyces in the prior art

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  • Streptomycete TJ561, product for preventing and controlling successive cropping obstacle of soil, preparation method and application thereof and application of eugenol
  • Streptomycete TJ561, product for preventing and controlling successive cropping obstacle of soil, preparation method and application thereof and application of eugenol
  • Streptomycete TJ561, product for preventing and controlling successive cropping obstacle of soil, preparation method and application thereof and application of eugenol

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preparation example Construction

[0053] In a preferred embodiment, the preparation method of the spore suspension comprises: Streptomyces TJ561 using the first solid medium, cultured upside down at 27-29°C for 3-5 days, collecting the spores into sterile water to obtain the spore suspension . The first solid medium includes: glucose 6-9g / L, peptone 1.5-3.5g / L, soybean meal powder 1.5-2.1g / L, yeast powder 1-2g / L and agar powder 17-23g / L, pH8.0 -8.5. Streptomyces TJ561 is solidly fermented on this medium, and the spore seed liquid required for fermentation can be obtained quickly and in large quantities, and the spores formed are of good quality.

[0054] Further preferably, the first solid medium includes: glucose 8g / L, peptone 2.5g / L, soybean meal powder 1.8g / L, yeast powder 1.5g / L and agar powder 20g / L, pH 8.0-8.5.

[0055] In a preferred embodiment, the fermentation conditions of Streptomyces TJ561 include: solid fermentation at 26-28° C. for 2-4 days.

[0056] Preferably, the second solid medium for sol...

Embodiment 1

[0061] Screening, identification and preservation of Streptomyces TJ561 of embodiment 1

[0062] (1) The strain TJ561 was isolated from vegetable soil in Baodi District, Tianjin. The collected field soil samples were dried in an oven at 100°C for 24 hours, taken out and cooled to room temperature at room temperature for later use.

[0063] (2) Preparation method of strain solid separation medium: Weigh 5.0g of glucose, 6.0g of starch, 5.0g of malt extract, 3.0g of yeast powder, 2.0g of peptone, 3.0g of ammonium sulfate, and 0.2g of calcium carbonate, and add 1.0L Fully dissolve the water, adjust the pH between 7.5-8.0 with 0.1M sodium hydroxide, then add 20g of agar powder, mix evenly, divide into 500ml triangular flasks according to the liquid volume of 200ml per bottle, and store at 121°C Sterilize under high temperature for 30 minutes, and when the culture is cooled to 50-60°C at room temperature, add 1.0ml of sterile nystatin aqueous solution with a concentration of 1000m...

Embodiment 2

[0069] The bacteriostasis of embodiment 2 Streptomyces TJ561

[0070] Indoor antimicrobial spectrum test of strain TJ561.

[0071] (1) Fermentation broth preparation: using the block-digging inoculation method, inoculate the mature strain TJ561 grown on a solid slant medium into a mixture containing glucose 15g / L, peptone 6g / L, yeast powder 4g / L, and calcium carbonate 0.2g / L , in a liquid medium with pH=7.5-8.0. Cultivate for 96 hours at 28°C with a rotational speed of 180 rpm. Centrifuge at 3000 rpm for 5 min, and take the supernatant for later use.

[0072] (2) The strain TJ561 has good antibacterial effect against various plant pathogenic fungi indoors. In the aseptic operation bench, use the inoculation loop to scrape the hyphae of the plant pathogenic fungi Botrytis cinerea, cabbage black spot, bean sclerotia, and Phytophthora capsici growing in the inclined test tube until it is filled with 20ml of sterile water and 20-25 Particle diameter is in the 50ml Erlenmeyer f...

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Abstract

The invention relates to the field of microbes, in particular to streptomycete TJ561, a product for preventing and controlling successive cropping obstacle of soil, a preparation method and application thereof and application of eugenol. The preservation number of the streptomyce TJ561 is CGMCC No.17247. The streptomycete TJ561 has the multiple effects of improving disease resistance of vegetables, inhibiting soil-borne fungal diseases, promoting crop growth and increasing crop yield. The streptomycete TJ561 has the good effect on improving the successive cropping obstacle of soil, and the product, for preventing and controlling the successive cropping obstacle of soil, prepared by using the strain can effectively inhibit diseases, promote the growth of crop root systems and increase the yield after being applied to soil, so that soil problems caused by perennial successive cropping can be solved, and intensive production development of modern agriculture is facilitated. The preparation method of the product for preventing and controlling successive cropping obstacle of soil is simple, easy to operate, low in cost and suitable for large-scale production.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a streptomyces TJ561, a product for preventing and controlling soil continuous cropping obstacles, a preparation method and application thereof, and an application of eugenol. Background technique [0002] Facility vegetable production is a form of land use with strong human influence, which has the characteristics of high input, high output, common continuous cropping, and often in a closed state. Continuous cropping of the same crop can enrich certain specific microbial populations, especially plant pathogenic fungi, which are not conducive to the balance of microbial populations in the soil, but are conducive to the occurrence of plant root diseases, reducing yield year by year. With the increase of continuous cropping years in greenhouse vegetable continuous cropping soil, the types and numbers of fungi decreased, but the types and numbers of harmful pathogenic fungi increased. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00A01N63/00A01P3/00A01P21/00C09K17/14C12R1/465C09K101/00
CPCC12N3/00A01N63/00C09K17/14C09K2101/00C12R2001/465C12N1/205
Inventor 刘红梅杨殿林赵建宁王慧张艳军
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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