Quality detection method of Yao medicinal herb Pilea cavaleriei subsp. cavaleriei

A quality inspection method and technology of petroleum vegetable, applied in the direction of measuring device, material separation, analysis material, etc., can solve the problems affecting the safety and effectiveness of clinical drug treatment, uncontrollable quality of medicinal materials, and lack of quality standards for medicinal materials, so as to improve safety. and stability, high sensitivity and good comparability

Inactive Publication Date: 2019-09-17
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no quality standard for the medicinal material, and the quality of the medicinal material cannot be controlled, resulting in large differences in the efficacy of folk or clinical medication, which seriously affects the safety and effectiveness of its clinical medication.

Method used

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  • Quality detection method of Yao medicinal herb Pilea cavaleriei subsp. cavaleriei

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Experimental program
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Effect test

Embodiment 1

[0027] A quality detection method of Yao medicine rapeseed medicinal material, comprising a TLC identification method of the rapeseed medicinal material and a method for determining the total flavonoid content in the rapeseed medicinal material, wherein the TLC identification method of the rapeseed medicinal material comprises the following steps:

[0028] (1) Preparation of the test solution: Weigh 1.0 g of petroleum vegetable medicinal powder, pass through a No. 2 sieve, add 30 mL of ethanol with a volume concentration of 95%, ultrasonically extract for 30 min, filter with suction, evaporate the filtrate to dryness, add 2 mL of the extraction solvent is dissolved to obtain the product; the same method is used for the preparation of the test solution of the reference medicinal material;

[0029] (2) Preparation of reference substance solution: Take an appropriate amount of β-sitosterol reference substance, add ethanol with a volume concentration of 95% to make a reference subs...

Embodiment 2

[0038] One, the investigation situation of the system adaptability of the TLC identification method of the petroleum vegetable medicinal material of the present invention is as follows:

[0039] Materials: β-sitosterol reference substance (batch number 110851-201608, China Institute for Food and Drug Identification). Reagents: methanol (Chengdu Kelong Chemicals Co., Ltd., 2017052601); ethanol (Chengdu Kelong Chemicals Co., Ltd., 2017102601); ethyl acetate (Chengdu Mao Chemical Reagent Factory, 20170102); Ether (Shanghai Wokai Biotechnology Co., Ltd., 20170919); water is pure water.

[0040] 1. Sample volume

[0041] Weigh 5 parts of 1 g of reference medicinal material powder (passed through a No. 2 sieve), add 30 mL of ethanol with a volume concentration of 95%, ultrasonicate for 30 minutes, filter with suction, evaporate the filtrate to dryness, and dissolve the residue in 2 mL of extraction solvent to obtain the test product product solution. Investigate the sample volume...

Embodiment 3

[0068] One, the methodological investigation of the assay method of the total flavonoid content in the petroleum vegetable medicinal material described in the quality detection method of Yao medicine petroleum vegetable medicinal material of the present invention is as follows:

[0069] 1. Drawing of standard curve

[0070] Accurately measure 0.2092 mg / mL rutin control solution 0, 1, 3, 5, 6, 8 mL respectively, add pure water to 8 mL, add 1 mL sodium nitrite, mix well, let stand for 6 min, add aluminum nitrate 1 mL, mix well, stand still for 6 min, slowly add 10 mL of sodium hydroxide dropwise within 5 min, and after standing for 10 min, conduct full-wavelength scanning under ultraviolet light, and measure the absorbance of each solution at 510 nm, and compare with rutin The concentration of the product solution (mg / mL) is the abscissa, and the absorbance at 510 nm is the ordinate to draw the working curve to obtain the regression equation.

[0071] For the drawing of rutin s...

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Abstract

The present invention belongs to the technical field of traditional Chinese medicine analysis, in particular to a quality detection method of Yao medicinal herb Pilea cavaleriei subsp. cavaleriei. The quality detection method comprises a TLC identification method of the medicinal herb Pilea cavaleriei subsp. cavaleriei and a method for determining a total flavonoid content in the medicinal herb Pilea cavaleriei subsp. cavaleriei. The TLC identification method of the medicinal herb Pilea cavaleriei subsp. cavaleriei is simple and feasible, has strong reproducibility and specificity, can be used for accurate authenticity judgement of the medicinal herb, and can directly respond to the quality of the medicinal herb to a certain extent. By using the method for determining the total flavonoid content in the medicinal herb Pilea cavaleriei subsp. cavaleriei, the total flavonoid content in the medicinal herb Pilea cavaleriei subsp. cavaleriei can be accurately and quickly determined, which facilitates the quality monitoring of the medicinal herb Pilea cavaleriei subsp. cavaleriei. The quality of the medicinal herb Pilea cavaleriei subsp. cavaleriei can be determined and evaluated more accurately with the quality detection method of the Yao medicinal herb Pilea cavaleriei subsp. cavaleriei of the invention, and the quality detection method is beneficial to improving the safety and stability of the medicinal herb Pilea cavaleriei subsp. cavaleriei.

Description

technical field [0001] The invention belongs to the technical field of analysis of traditional Chinese medicines, and in particular relates to a method for detecting the quality of medicinal materials of Yao medicine and petroleum vegetable. Background technique [0002] Yaoyao oleracea is Urticaceae plant with corrugated cold water flowers Pilea cavaleriei H. Lév. The whole plant, aliased with stone cauliflower, fat slave grass, small stone mustard, frozen grass, malnutrition grass, stone amaranth, fat pig dish, beaten dead, stone cold dish, frog legs, distributed in Guangxi, Guangdong, Guizhou, Hunan and other places . Born in the shade of rocky mountains, more common on rocks under forests on hillsides at an altitude of 300-1500 meters. Harvested throughout the year, washed, used fresh or sun-dried. Slightly bitter, cool in nature; returns lung, spleen meridian. Clearing lung heat and relieving cough, inducing diuresis to reduce swelling, detoxifying and relieving pai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/95
CPCG01N30/90G01N30/95
Inventor 王志萍陈晓艺庞宇舟黎芳李晓霞蒋雅娴刘笑苏佳昇
Owner GUANGXI UNIV OF CHINESE MEDICINE
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