Low-temperature resistant cellulose penicillium and application thereof

A Penicillium, cellulase technology, applied in the application, enzyme, hydrolase and other directions, to achieve the effects of high β-glucosidase activity, good environmental and economic benefits, and good industrial application prospects

Inactive Publication Date: 2019-09-20
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of low-temperature microbial strain resources, the research on the microbial transformation of cellulose is mainly concentrated at room temperature or high temperature, and there are relatively few studies on low-temperature strains and their enzyme-producing ability.

Method used

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  • Low-temperature resistant cellulose penicillium and application thereof
  • Low-temperature resistant cellulose penicillium and application thereof
  • Low-temperature resistant cellulose penicillium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The separation and identification of embodiment 1 Penicillium NLS-2

[0037] Please refer to figure 1 , figure 1 A flow chart for the preparation of the low-temperature resistant Penicillium cellulolyticum NLS-2 provided by the embodiment of the present invention is shown. The preparation steps of the low-temperature-resistant Penicillium cellulolyticum NLS-2 provided by the embodiments of the present invention are:

[0038] Collect soil samples from the rhizosphere of Prunus japonica at an altitude of 4500m in the Nagqu region of Tibet; put the collected soil samples into a centrifuge tube filled with sterile water and shake them fully to make a soil suspension; dilute the soil suspension gradient to make 10 -2 to 10 -7 concentration of soil suspension; add the diluted soil suspension to the PDA medium plate for coating treatment, and put it in a 4°C incubator for cultivation to obtain colonies; select single colonies with different shapes for purification, and cult...

Embodiment 2

[0048] Determination of cellulose degrading ability under the low temperature of embodiment 2 Penicillium strain NLS-2

[0049] The embodiment of the present invention provides a schematic diagram of the hydrolysis effect of the low-temperature-resistant Penicillium cellulolyticum strain NLS-2 on a Congo red sodium carboxymethylcellulose plate, see Image 6 . from Image 6 It can be seen that at 15°C, the Congo red carmellose sodium plate inoculated with Penicillium strain NLS-2 had obvious hydrolysis circles after 5 days, and the diameter of the hydrolysis circles was above 17.4 mm, which was caused by the appearance of hydrolysis circles. It can be shown that the Penicillium strain NLS-2 provided in the examples of the present invention has a strong potential to degrade cellulose under low temperature conditions.

Embodiment 3

[0050] Example 3 Determination of Cellulase Production Ability of Penicillium Strain NLS-2 at Low Temperature

[0051] The embodiment of the present invention has carried out cellulase-producing ability assay to Penicillium bacterial strain NLS-2, and this assay includes the following contents:

[0052] 1. Preparation of crude enzyme solution

[0053] The bacterial strain NLS-2 provided by Example 1 of the present invention was inoculated into PDA liquid medium, cultivated at 15°C, 160r / min for 24h, and then inoculated with 2% inoculum in the enzyme-producing medium, at 15°C, 160r / min / min for 7 days. During this period, the enzyme activity was measured every 24 hours, and the fermentation broth was taken at 6000r / min, 4°C, centrifuged for 10min, and repeated three times at each time, and the supernatant was the crude enzyme solution.

[0054] The composition of described enzyme production medium is: CMC-Na 15.0g / L, yeast powder 5.0g / L, KH 2 PO 4 2.0g / L, pH7.0, sterilized...

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Abstract

The invention provides a low-temperature resistant cellulose penicillium and an application thereof. The preservation number of penicillium NLS-2 (Penicillium sp. NLS-2) is CCTCC NO: M20154. The penicillium strain NLS-2 has strong cellulase production capacity at 15 DEG C, and the cellulase is endo-beta-glucanase, exo-beta-glucanase and beta-glucosidase respectively, and thus the penicillium strain NLS-2 has the activity of regulating soil functional enzyme, so that the penicillium has a good industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of bacterial strain isolation and application, in particular to a low-temperature-resistant Penicillium cellulolyticum and its application. Background technique [0002] Cellulose is a macromolecular polysaccharide composed of glucose connected by β-1,4-glucosidic bonds. It is an important component of plant cell walls, accounting for about 35%-50% of plant dry weight, and is the most abundant carbohydrate in nature. compound. At the same time, cellulose is also a very important renewable resource, which can be used as a raw material for energy, food and chemical industries, such as the production of ethanol, lactic acid and single-cell protein. However, because cellulose contains a large number of high-energy hydrogen bonds, it is not easy to be degraded, and the utilization rate is low. Most of the cellulose resources are discarded or directly incinerated, resulting in extremely serious resource waste and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C09K17/14C12R1/80
CPCC09K17/14C12N9/2437C12Y302/01004C12Y302/01074C12Y302/0115C12N1/145C12R2001/80
Inventor 黄艺张梦君邱晨浩柴立伟
Owner PEKING UNIV
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