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Preparation method of wild antheraea pernyi silk fibroin/aloe gel composite dressing

A technology of tussah silk fibroin and aloe vera gel, which is applied in the field of preparation of tussah silk fibroin/aloe vera gel composite dressing, can solve the problem that the speed and effectiveness of collagen wound repair are not as fast as aloe vera gel, and wound ulceration is not very effective. Composite scaffolds have the effect of enhancing cell adhesion and growth, promoting cell adhesion, and repairing wounds due to the high cost of composite scaffolds

Active Publication Date: 2019-09-24
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent with application number 200610155970.4 uses natural tussah silk as raw material, and adopts electrospinning method to obtain tussah silk fibroin protein fiber felt porous material. The repair of scrapes, cuts, wound ulcers, etc. is not very effective
The Chinese patent application number 201810344414.4 proposes a method of preparing a collagen / tussah silk fibroin composite scaffold loaded with BMSCs by freeze-drying method. The prepared scaffold has good biocompatibility, large pore size and no inflammation Reaction, immune rejection, etc., strong cell adhesion, can promote skin wound repair, but because the cost of collagen is high, and the preparation method of BMSCs suspension is responsible, resulting in high cost of preparation method of composite scaffold, collagen has no effect on wound The speed and effectiveness of repair are not as fast as aloe vera gel

Method used

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  • Preparation method of wild antheraea pernyi silk fibroin/aloe gel composite dressing
  • Preparation method of wild antheraea pernyi silk fibroin/aloe gel composite dressing
  • Preparation method of wild antheraea pernyi silk fibroin/aloe gel composite dressing

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Experimental program
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Effect test

Embodiment 1

[0027] With 0.5~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming tussah silk, and then dissolve it with 5~10% lithium thiocyanate (LiSCN) aqueous solution, and after centrifugation, suction filtration, and dialysis, the regenerated tussah fibroin protein aqueous solution is obtained, and the obtained regenerated tussah silk fibroin The protein aqueous solution was concentrated until the mass percentage of tussah silk fibroin protein was 5-20%, which was recorded as solution A.

[0028] The aloe gel is crushed with a pulverizer, and the aloe gel powder is prepared by freeze-drying method. The aloe gel powder is dissolved in water to prepare a 1-10% aloe gel aqueous solution. The freezing temperature of the freeze-drying method is -80 ~ -20°C, the freezing time is 48 h.

[0029] Take solution A with 20% tussah fibroin protein mass percentage and 2% aloe vera gel aqueous solution for blending and stirring, and control the mass ratio of aloe vera gel and tussah fib...

Embodiment 2

[0034] The difference between embodiment 2 and embodiment 1 is: embodiment 2 controls the mass ratio of aloe vera gel and tussah silk protein to be 1:50, and other operations are the same as embodiment 1, as follows:

[0035] With 0.5~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming tussah silk, and then dissolve it with 5~10% lithium thiocyanate (LiSCN) aqueous solution, and after centrifugation, suction filtration, and dialysis, the regenerated tussah silk fibroin protein aqueous solution is obtained, and the obtained regenerated tussah silk The protein aqueous solution is concentrated until the mass percentage of tussah silk fibroin protein is 5-20%, which is recorded as solution A.

[0036] The aloe gel is crushed with a pulverizer, and the aloe gel powder is prepared by freeze-drying method. The aloe gel powder is dissolved in water to prepare a 1-10% aloe gel aqueous solution. The freezing temperature of the freeze-drying method is -80 ~ -20°C, the freezi...

Embodiment 3

[0042] The difference between embodiment 3 and embodiment 1 and embodiment 2 is: embodiment 3 controls the mass ratio of aloe vera gel and tussah silk protein to be 1:10, and other operations are the same as embodiments 1 and 2, as follows:

[0043] With 0.5~1% sodium carbonate (Na 2 CO 3 ) aqueous solution to degumming tussah silk, and then dissolve it with 5~10% lithium thiocyanate (LiSCN) aqueous solution, and after centrifugation, suction filtration, and dialysis, the regenerated tussah silk fibroin protein aqueous solution is obtained, and the obtained regenerated tussah silk The protein aqueous solution is concentrated until the mass percentage of tussah silk fibroin protein is 5-20%, which is recorded as solution A.

[0044] The aloe gel is crushed with a pulverizer, and the aloe gel powder is prepared by freeze-drying method. The aloe gel powder is dissolved in water to prepare a 1-10% aloe gel aqueous solution. The freezing temperature of the freeze-drying method is ...

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Abstract

The invention relates to the field of a biomedical material and discloses a preparation method of wild antheraea pernyi silk fibroin / aloe gel composite dressing. The preparation method comprises the following steps of (1) after tussah silk is degummed, enabling the degummed tussah silk to dissolve to prepare a regenerated wild antheraea pernyi silk fibroin aqueous solution; (2) enabling aloe gelatin powder to dissolve in water to prepare an aloe gel solution in a certain proportion; (3) blending the aloe gel solution with the wild antheraea pernyi silk fibroin aqueous solution, controlling the mass ratio of the aloe gel to the wild antheraea pernyi silk fibroin being (1 to 10) to (1 to 100), and continuing to concentrate the mixed solution until the wild antheraea pernyi silk fibroin achieves a certain concentration; (4) preparing a two-dimensional fibrous framework and a three-dimensional spongy porous framework respectively through an electrostatic spinning method and a freeze-drying method; and (5) treating the composite material with ethanol water. Compared with the prior art, the preparation method is simpler, easier to operate and lower in cost, the wild antheraea pernyi silk fibroin / aloe gel composite dressing can be effectively prepared by the preparation method, and the preparation method can be used as wound dressing.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of tussah silk fibroin / aloe gel composite dressing. Background technique [0002] Silk is a common and inexpensive biomaterial with excellent flexibility, breathability, biodegradability, and biocompatibility. Silk fibroin accounts for about 70-85% of the total mass of silk. Because it is non-toxic to cells and the body, it will not or less cause inflammation and immune rejection, so it has great application value in the field of biomedicine. Tussah silk is rich in content, and my country's annual tussah silk production accounts for about 90% of the world's total output. Tussah silk fibroin contains a unique arginine-glycine-aspartic acid (RGD) tripeptide sequence, and the RGD sequence is more conducive to cell adhesion and proliferation. Therefore, compared with silkworm silk fibroin, tussah silk fibroin The healing effect is more significant....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00A61L15/32A61L15/40A61L15/42A61L15/44A61L15/46
CPCA61L15/32A61L15/40A61L15/425A61L15/44A61L15/46A61L26/0028A61L26/0057A61L26/0066A61L26/0085A61L2300/252A61L2300/30A61L2300/404A61L2300/412C08L89/00
Inventor 张超王心如叶玮丁红燕
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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