Microorganism composite bacterium agent capable of preventing and treating grape powdery mildew and preparation method of microorganism composite bacterium agent

Abstract

The invention discloses a microorganism composite bacterium agent capable of preventing and treating grape powdery mildew and a preparation method of the microorganism composite bacterium agent. The microorganism composite bacterium agent comprises bacillus subtilis, trichoderma harzianum and lactobacillus plantarum, wherein the biological preservation number of the bacillus subtilis is CCTCC NO:M 2018854. The preparation method comprises the steps of performing deep fermentation on the bacillus subtilis, the trichoderma harzianum and the lactobacillus plantarum, adding an adsorbent in proportion, and then performing uniform mixing so as to obtain the microorganism composite bacterium agent. The microorganism composite bacterium agent provided by the invention shows obvious synergistic effects in a certain compounding ratio range and has excellent preventing and treating effects on the grape powdery mildew, the number of times of medication and the medication cost are reduced, and the microorganism composite bacterium agent is green and pollution-free; the medicine effects are rapid to exert, the preventing and treating effects are excellent, the persistent efficacy period is long, and medicine resistance cannot be produced; and the microorganism composite bacterium agent can coordinate and cooperate with other fruit tree protecting measures to jointly exert effects, and has great application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological control, relates to the technical field of microbial bacterial agents for plant disease control, and more specifically relates to a microbial composite bacterial agent for preventing and treating grape powdery mildew and a preparation method thereof. Background technique [0002] Grape powdery mildew is a fungal disease, and the pathogen is hookworm. The pathogen survives the winter in the affected tissue or bud scales as mycelium, and produces conidia in the second spring, which are spread by wind and rain and penetrate the epidermis for initial infection. Reinfestation can be done multiple times during the growing season. Dry summer and warm, humid, and hot weather are conducive to the occurrence of powdery mildew. Generally, the onset begins in June, peaks from mid-to-late July to early August, and stops in September-October. [0003] Grape powdery mildew mainly damages the leaves, grape tr...

AI Technical Summary

Problems solved by technology

[0004] At present, the control of grape powdery mildew is mainly based on chemical control, such as spraying Baume 3-5% lime sulfur mixture or 45% crystal lime sulfur mixture 40-50 times before the grape buds swell and germinate. Spray Bordeaux mixture once a day, and spray 2-3 times continuously for prevention; at the early stage of the disease, spray for prevention and control, 10% flusilazole 1500 times spray, 70% thiophanate-methyl WP 1000 times liquid or pyrimethol control White 800 times solution, etc. However, due to long-term and large-scale use, grape powdery mildew bacteria have produced varying degrees of drug resistance. The annual use of pesticides has seriously exceeded the tolerance of cultivated land, and it is also easy to cause great pollution to groundwater.

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