Seed initiator preparation method

An initiator and seed technology, applied in the field of seed initiator preparation, can solve problems such as heavy workload, difficult production, and long cycle, and achieve the effects of convenient operation, increased yield, and accelerated accumulation

Active Publication Date: 2019-10-08
桐乡市杭吉生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are three methods to improve plant resistance to temperature stress. The first is to cultivate varieties resistant to temperature stress. However, this method takes a long time and is difficult to be directly applied to production.
The second is to strengthen the cultivation management. Although this method can improve the temperature and adversity resistance of rice to a certain extent, the workload is very large.

Method used

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preparation example Construction

[0033] The above preparation method of Bacillus subtilis fermentation can increase the acetylation level of histones, promote transcriptional activation, cause an increase in transcription level, regulate the selective expression of genes without changing the genomic DNA sequence, and promote the production of Bacillus subtilis. Versatility-related gene expression can increase the yield of metabolites, and the fermentation product of Bacillus subtilis contains a large amount of low-molecular-weight γ-polyglutamic acid (10-1200KD), which has a high water absorption and water retention function and can chelate multiple Mineral ions, improve the antioxidant enzyme activity of seeds, reduce the degree of lipid peroxidation of cell membranes, improve the cold tolerance of rice, can promote subsequent seed germination and seedling growth and development, and lay the foundation for strong seedlings and high yield; at the same time, it can activate subtilis The expression of silencing ...

Embodiment 1

[0052] The preparation method of seed initiator, comprises the steps:

[0053] 1) Inoculate the Bacillus subtilis seed liquid into the fermentation medium containing glutamic acid, methylene salicylic acid bacitracin and trichostatin A according to the inoculum size of 5% (V / V), at 30 ° C, Shake culture at constant temperature at 200r / min for 20h, dry to obtain Bacillus subtilis fermentation product, wherein the fermentation medium contains 80g / L molasses, 20g / L soybean hydrolyzate, 0.05g / L bacitracin methylene salicylate, 4g / L L yeast extract, 1g / L trichostatin A, 8g / L sodium citrate, 5g / L glutamic acid, 2g / L valine, 5g / L ammonium sulfate, 0.2g / L MgSO 4 , 10g / L KH 2 PO 4 ;

[0054] 2) Enzymolyze, centrifuge, and filter the rice bran protein to obtain a compound amino acid solution. The concentration of the concentrated amino acid aqueous solution is 12%, and then neutralize it with a mixed solution with a concentration of 23% and sodium ethoxide and sodium hydroxide in a w...

Embodiment 2

[0057] The preparation method of seed initiator, comprises the steps:

[0058] 1) Inoculate the Bacillus subtilis seed liquid into the fermentation medium containing glutamic acid, methylene salicylic acid bacitracin and trichostatin A according to the inoculation amount of 7% (V / V), at 35 ℃, Shake culture at a constant temperature of 250r / min for 24 hours, and dry to obtain a Bacillus subtilis fermentation product, wherein the fermentation medium contains 100g / L molasses, 35g / L soybean hydrolyzate, 0.08g / L bacitracin methylene salicylate, 5g / L L yeast extract, 2g / L trichostatin A, 10g / L sodium citrate, 7g / L glutamic acid, 3g / L valine, 8g / L ammonium sulfate, 0.4g / L MgSO 4 、15g / L KH 2 PO 4 ;

[0059]2) Enzymatically hydrolyzing rice bran protein, centrifuging, and filtering to obtain a compound amino acid solution. The concentration of the concentrated amino acid aqueous solution is 15%, and then neutralized with a mixed solution with a concentration of 25% and sodium ethoxi...

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Abstract

The invention provides a seed initiator preparation method, which includes adding N-cocoyl compound amino acid, Bacillus subtilis fermentation product, vitamin E, Lanthanum chlorideheptahydrate, salicylic acid and gamma-aminobutyric acid into a blending kettle and mixing to prepare the seed initiator, wherein the Bacillus subtilis fermentation product is prepared by the following process: inoculating a Bacillus subtilis seed liquid into a fermentation medium containing glutamic acid, methylene salicylic acid bacitracin and trichostatin A, culturing, and drying to obtain the Bacillus subtilis fermentation product. The preparation method promotes Bacillus subtilis to metabolize more gamma-polyglutamic acid and new substances, and improves the yield of N-cocoyl compound amino acid; the stability, slow release and shelf life of the seed initiator can be improved, the obtained seed initiator can improve the seed germination ability and the seed germination rate, remarkably improve the tolerance of the obtained rice seedlings to drought stress and high salt stress and the accumulation of lysine, and improve the nutritional value of the obtained rice.

Description

technical field [0001] The invention belongs to the technical field of seed treatment, and in particular relates to a preparation method of a seed initiator. Background technique [0002] There are three commonly used temperature indicators for plants, namely optimum temperature, minimum temperature, maximum temperature, damage temperature and lethal temperature. The first three temperatures are referred to as the three base point temperatures. Temperature stress refers to the temperature and environmental conditions that are unfavorable to plant life activities, exceeding the three-point temperature of the plant itself. Its damage to plants can be divided into direct damage and indirect damage. Direct damage is an irreversible damage to plant life structure proteins and nucleic acids in a short period of time, such as high temperature burns and freezing. Indirect damage is a long-term effect that causes metabolic damage. disorder, causing permanent damage. Low temperatur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01N59/16A01N37/44A01N37/40A01N43/16A01N63/02A01P21/00C12N1/20C12R1/125
CPCA01C1/00A01N59/16A01N37/44A01N37/40A01N43/16C12N1/20A01N63/10
Inventor 周艳初
Owner 桐乡市杭吉生态农业科技有限公司
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