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Temperature-sensitive adhesive decalcified-bone composite tissue engineering support and preparation method thereof

A tissue engineering and composite tissue technology, applied in the field of tissue engineering materials, demineralized bone composite tissue engineering scaffolds and their preparation, can solve the problems of large pore size of demineralized bone, single cells, unable to load cells, etc., and achieve the promotion of bone tissue. Repair, improve adhesion strength, enhance the effect of adhesion

Inactive Publication Date: 2019-10-25
尹静波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the decalcified bone materials and decalcified bone composite tissue engineering scaffolds reported in the existing literature or patents still have the following defects: 1) the pore size of the decalcified bone is large, and an effective number of cells cannot be loaded; 2) the existing decalcified bone The loaded cells are single, and cell co-culture cannot be carried out, so bone tissue repair cannot be accelerated. 3) The decalcified bone material lacks the function of integrating with surrounding normal tissues

Method used

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  • Temperature-sensitive adhesive decalcified-bone composite tissue engineering support and preparation method thereof

Examples

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Embodiment 1

[0050] Example 1: Preparation of a Thermosensitive Adhesive Decalcified Bone Composite Tissue Engineering Scaffold I

[0051] 1g of low-viscosity chitosan powder was soaked in 10mL of 50% sodium hydroxide aqueous solution under nitrogen protection and room temperature for alkalization treatment, after alkalization treatment for 6h, filtered to remove sodium hydroxide aqueous solution, and added 10mL of 50% isopropanol aqueous solution After the chitosan was uniformly dispersed in the isopropanol aqueous solution, 20 mL of butylene oxide was added, and after reflux reaction at 60°C for 12 hours, the product was dialyzed and freeze-dried to obtain low-viscosity HBC. Dissolve low-viscosity HBC in deionized water, add EDC / NHS activation system according to the molar ratio of HBC:EDC:NHS=1:2:1, and then add LDA according to the molar ratio of HBC:LDA=1:0.05 to make it on the HBC side The chain grafting rate was 5%, reacted at room temperature for 2 days, and freeze-dried to obtain ...

Embodiment 2

[0053] Example 2: Preparation of a Thermosensitive Adhesive Decalcified Bone Composite Tissue Engineering Scaffold II

[0054] 1g of medium-viscosity chitosan powder is soaked in 10mL of 50% sodium hydroxide aqueous solution under nitrogen protection and room temperature for alkalization treatment, after alkalization treatment for 12h, the sodium hydroxide aqueous solution is removed by filtration, and 10mL of 50% isopropanol aqueous solution is added After the chitosan was uniformly dispersed in the isopropanol aqueous solution, 20 mL of butylene oxide was added, and after reflux reaction at 60° C. for 16 h, the product was dialyzed and freeze-dried to obtain medium-viscosity HBC. Dissolve medium-viscosity HBC in deionized water, add EDC / NHS activation system according to the molar ratio of HBC:EDC:NHS=1:2:1, and then add LDA according to the molar ratio of HBC:LDA=1:0.1 to make it on the HBC side The chain grafting rate was 10%, reacted at room temperature for 2 days, dialyz...

Embodiment 3

[0056] Example 3: Preparation of a Thermosensitive Adhesive Decalcified Bone Composite Tissue Engineering Scaffold III

[0057] 1g of high-viscosity chitosan powder is soaked in 10mL of 50% sodium hydroxide aqueous solution under nitrogen protection and room temperature for alkalization treatment, after alkalization treatment for 24h, the sodium hydroxide aqueous solution is removed by filtration, and 10mL of 50% isopropanol aqueous solution is added After the chitosan was uniformly dispersed in the aqueous solution of isopropanol, 20 mL of butylene oxide was added, and after reflux reaction at 60° C. for 18 hours, the product was dialyzed and freeze-dried to obtain high-viscosity HBC. Dissolve high-viscosity HBC in deionized water, add EDC / NHS activation system according to the molar ratio of HBC:EDC:NHS=1:2:1, and then add LDA according to the molar ratio of HBC:LDA=1:0.15 to make it on the HBC side The chain grafting rate was 15%, reacted at room temperature for 2 days, and...

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Abstract

The invention discloses a temperature-sensitive adhesive tissue engineering material and a decalcified-bone composite tissue engineering support and a preparation method and application thereof. The levodope modified temperature-sensitive hydrogel preparation tissue engineer material and the corresponding composite tissue engineering support are used to the improvement of multiple performances: 1), carrying a large number of cells to facilitate co-culture of cells; 2), supporting protein or growth factors; 3), enhancing the adhesion to the tissue. The decalcified-bone support and the hydrogencan support different cells in use, so that the defect that the number of the loaded cells is affected due to the large pore size of the decalcified bone is compensated, co-culture of the cells can beconveniently realized, and the hydrogel can also carry proteins and the growth factors to further promote bone tissue repair; the introduction of levodopa in the hydrogel significantly enhance the adhesion between the tissue engineering support and the tissue, and the composite support is increased significantly to reach 11-18KPa.

Description

technical field [0001] The invention belongs to the field of bone repair, and in particular relates to a temperature-sensitive adhesive tissue engineering material, a decalcified bone composite tissue engineering scaffold, a preparation method and an application thereof. Background technique [0002] Trauma, infection, tumor, surgical debridement of osteomyelitis, and various congenital diseases are the main causes of bone defects. The current clinically used autologous bone grafts, allogeneic bone grafts, and bone implant materials cannot achieve ideal treatment As a result, the emergence of tissue engineering technology provides an ideal treatment strategy for bone defect reconstruction. [0003] A large number of bone tissue engineering studies have shown that to achieve an ideal bone defect repair should meet three requirements: 1) The material can be loaded with a large number of cells. Yaqian Li from Tsinghua University believes that an effective amount of cell loadin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/52A61L27/54A61L27/20
CPCA61L27/20A61L27/3608A61L27/40A61L27/52A61L27/54A61L2300/414C08L5/08
Inventor 尹静波
Owner 尹静波
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