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Bacillus subtilis strain and applications thereof

A technology of Bacillus subtilis and strains, applied in the field of applied biology, can solve the problems of single microbial treatment effect, etc., and achieve the effects of wide application range, avoiding potential safety hazards and light specific gravity

Active Publication Date: 2019-11-12
浙江亲水园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem of single microbial treatment effect for septic tank treatment in the prior art, the present invention provides a There is Bacillus subtilis QS1 with better decomposing ability, further the present invention provides a kind of preparation that utilizes Bacillus subtilis QS1 to carry out aerobic treatment

Method used

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  • Bacillus subtilis strain and applications thereof
  • Bacillus subtilis strain and applications thereof
  • Bacillus subtilis strain and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The present embodiment provides the screening and isolation method of the Bacillus subtilis bacterial strain of the present invention, and described screening method is as follows:

[0048] 1) Soil samples were taken in Hulunbuir Grassland, Inner Mongolia, and the flora in the soil samples were extracted;

[0049] 2) Cultivate soil flora in the Beijing laboratory to confirm that the soil flora has a good degradation effect on ammonia nitrogen, nitrite, COD, etc. in soil and sewage;

[0050] 3) Screen out the main bacilli in the soil flora, and reconfirm its excellent treatment effect on soil and sewage;

[0051] 4) The strain identification of the bacilli was carried out, and the result proved that the strain was Bacillus subtilis, the classification: Bacillus Subtilis, and the bacterial strain was QS1. The strain identification method is as follows: Genomic DNA extraction and molecular identification, comprehensive analysis of experimental data such as cell morphology...

Embodiment 2

[0057] The method of utilizing the Bacillus subtilis strain of the present invention to prepare bacterial agent of the present embodiment comprises the following steps:

[0058] 1) Take a small amount of pure bacteria from the slant of the preserved Bacillus subtilis QS1, and inoculate it on a plate for activation;

[0059] 2) use small test equipment to carry out fermentation experiment, adopt LB culture medium to complete fermentation under the condition of 37 ℃ of shaker culture for 12 hours; During the experiment, the type of medium and culture conditions can also be adjusted according to the experiment;

[0060] 3) Put the fermented bacterial liquid on the spray tower for spray drying, and the spray carrier uses natural perlite powder with a diameter of about 10 μm;

[0061] 4) After the sprayed bacterial powder is directly mixed with maltodextrin, it is packed into a bacterial agent with a content of 30g / bag, and the effective number of viable bacteria containing the Ba...

Embodiment 3

[0075] The method utilizing the bacillus subtilis bacterial strain of the present embodiment to prepare bacterial agent, comprises the following steps:

[0076] 1) Take a small amount of pure bacteria from the slant of the preserved Bacillus subtilis QS1, and inoculate it on a plate for activation;

[0077] 2) use small test equipment to carry out fermentation experiment, adopt LB culture medium to complete fermentation under 37 DEG C of shaking table culture for 24 hours; During the experiment, the type of medium and culture conditions can also be adjusted according to the experiment;

[0078] 3) Put the fermented bacterial liquid on the spray tower for spray drying, and use 100 μm natural perlite powder as the spray carrier;

[0079] 4) Mix the sprayed bacterial powder with maltodextrin and compound biological enzyme freeze-dried powder successively, and pack it into 30g / package of microbial agent, the effective number of viable bacteria containing Bacillus subtilis strain ...

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Abstract

The invention discloses a bacillus subtilis strain and applications thereof, and belongs to the technical field of applied biology. The strain is the bacillus subtilis strain QS1 and assigned the accession number CGMCCNO. 17314. A bacillus subtilis preparation is simultaneously disclosed. The preparation includes the bacillus subtilis strain QS1, natural perlite powder and maltodextrin; and a preparation process takes the natural perlite powder as a bacillus subtilis spray carrier, so that aerobic biochemical reaction on the bacillus subtilis in a septic tank can be conveniently performed. Thebacillus subtilis strain QS1 has good decomposition capability for proteins, fibrous substances and oil substances; and the strain can effective remove stink by applying to the septic tank and sewagetreatment, so that the problems of sludge and scum blocking can be effectively prevented, the occurrence of methane and hydrogen sulfide gas can be reduced, and potential safety hazards can be removed.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and more specifically relates to a bacillus subtilis strain and its application. Background technique [0002] The septic tank is a device for treating feces and filtering and sedimenting. The principle is that the solidified matter decomposes at the bottom of the pool, and the hydrated objects on the upper layer enter the pipeline to flow away, preventing the pipeline from being blocked, and giving enough solidified objects (feces and other garbage) Hydrolyzed over time. A septic tank refers to a small treatment structure that separates and settles domestic sewage and performs anaerobic digestion of sludge. [0003] As a traditional environmental protection facility with a history of more than 100 years, septic tanks have played an important role. However, with the progress of society and the development of urban modernization, there have also been problems that deviate from social developm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F11/04C12R1/125
CPCC12N1/20C02F3/34C02F11/04C12R2001/125C12N1/205Y02E50/30Y02W10/10
Inventor 丁庆曾筱芳
Owner 浙江亲水园生物科技有限公司
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