Biological adhesive based on biological engineering protein and preparation method of biological adhesive

A bioadhesive, bioengineering technology, applied in the preparation of protein adhesives, animal glue or gelatin, adhesive types, etc., can solve the lack of non-invasive technology, fixation and bone atrophy, poor bone healing, etc. problems, achieve the effects of no special equipment, low cost, good biocompatibility and degradability

Active Publication Date: 2019-11-15
BEIJING SHUNXING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modulus mismatch between hard bone material and bone tissue leads to localized stresses for fixation and bone atrophy
Metal grafts are ancillary vehicles that do not chemically react with bone and are often affected by aseptic relaxation, resulting in poor bone healing
Despite these shortcomings, suturing remains the gold standard for tissue reconstruction due to the lack of non-invasive techniques capable of surpassing them
On the other hand, our daily life experience shows that conventional adhesives exhibit strong adhesion in a dry environment, but are easily destroyed in the presence of water

Method used

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  • Biological adhesive based on biological engineering protein and preparation method of biological adhesive
  • Biological adhesive based on biological engineering protein and preparation method of biological adhesive
  • Biological adhesive based on biological engineering protein and preparation method of biological adhesive

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preparation example Construction

[0033] The present invention provides a kind of preparation method of bioadhesive based on bioengineering protein, comprises the following steps:

[0034] A) respectively dissolving the positively charged elastin-like protein and the alkyl-type anionic surfactant in ultrapure water to obtain an elastin-like protein solution and a surfactant solution;

[0035] The alkyl anionic surfactant comprises sodium dodecylbenzenesulfonate and / or sodium dodecylsulfonate;

[0036] B) adding the surfactant solution dropwise into the elastin-like protein solution, and mixing uniformly to obtain a binder solution;

[0037] C) centrifuging the adhesive solution and then freeze-drying to obtain a bioadhesive.

[0038] In the present invention, the type, source and amount of the positively charged elastin-like and alkyl anionic surfactants are the same as those of the above-mentioned positively charged elastin-like and alkyl anionic surfactants. The types, sources and dosages are the same and ...

Embodiment 1

[0046] Transform the positively charged elastin-like ELP vector plasmid into Escherichia coli expression strain BL21DE3, pick a single clone colony, and culture overnight with LB culture medium; add the overnight activated expression seed liquid to TB medium until the bacterial liquid reaches OD600 When the value is 0.8, the inducer isopropyl-β-D-thiogalactopyranoside is added and the temperature is lowered to 30 degrees for overexpression. After induction for 12 hours, collect the bacterial cells, resuspend them with lysis buffer, add protease inhibitors, DNase, and lysozyme, break the bacterial cells with a high-pressure crusher, centrifuge at a speed of 10,000 rpm or more, collect the supernatant, and purify by HPLC. Charged elastin-like ELP.

[0047] Take 11 mg of ELP protein and 7.5 mg of sodium dodecylbenzenesulfonate, and add them into 50 μL of ultrapure water respectively. The surfactant aqueous solution was added dropwise to the ELP protein aqueous solution, shaken a...

Embodiment 2

[0051] Take 8.7 mg of the ELP protein prepared in Example 1 and 6 mg of sodium dodecylbenzenesulfonate, and add them into 50 μL of ultrapure water respectively. The surfactant aqueous solution was added dropwise to the ELP protein aqueous solution, shaken and centrifuged for 10 minutes, and the precipitate layer was left to freeze-dry for 20 minutes to obtain the ELP adhesive.

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Abstract

The invention provides a biological adhesive based on a biological engineering protein and a preparation method of the biological adhesive. The biological adhesive consists of an elastin-like polypeptide with positive charges and an alkyl anion surface active agent. The alkyl anionic surfactant comprises sodium dodecyl benzene sulfonate and / or sodium dodecyl sulfonate. According to the invention,the elastin-like polypeptide (ELP) containing a large amount of positive charges is synthesized through a recombinant protein technology, and then the side chain of the polypeptide skeleton is combined with the anionic surfactant through electrostatic acting force, so that the biomedical adhesive with high adhesive strength is prepared. The obtained product has excellent adhesive performance underdifferent conditions (dry sample, wet sample and animal epidermis), and can be used for hemostasis and wound healing. Meanwhile, the biomedical adhesive has good biocompatibility and degradability, the preparation process is simple, special equipment is not needed, cost is low, greenness and environment friendliness are achieved, and the biomedical adhesive can be developed into a new generationof biomedical glue.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a high-strength bioadhesive based on bioengineering proteins and a preparation method thereof. Background technique [0002] An adhesive is a material used to bond two or more surfaces together under defined conditions. It plays an important role in the fields of high technology and biomedicine. Likewise, in nature, adhesion across multiple length scales is essential for survival. Inspired by nature, researchers have synthesized various biomimetic adhesives through the study of various adhesion mechanisms of biological organisms. However, these adhesives usually have a long synthesis cycle and are expensive, and some adhesives need to be glued by ultraviolet radiation, which greatly limits the application of existing adhesives in the fields of medicine and industry. Worldwide a large number of surgical procedures are performed every year, in a recent study over 300 millio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J189/00C09J11/06
CPCC09J11/06C09J189/00C08K5/42
Inventor 刘凯马超孙静张洪杰
Owner BEIJING SHUNXING BIOTECH CO LTD
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