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Bacillus amyloliquefaciens culture, preparation method and application thereof

A technology for cultivating amyloliquefaciens and bacilli, which is applied in the field of culture and preparation of amyloliquefaciens bacillus, can solve problems such as piglet diarrhea, boar sperm deformity, distiller's grains are hot, etc., to improve economic value and prevent animal diarrhea , the effect of promoting growth rate

Active Publication Date: 2019-11-15
HUAZHONG AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Feeding practice has proved that feeding sows with fresh grains containing rice husks will reduce the quality of sow milk and cause diarrhea in piglets; Symptoms such as rapid heartbeat and vasoconstriction affect the normal development of pigs; in addition, distiller's grains are hot and not suitable for feeding in summer
It can be seen that although fresh distiller's grains are rich in nutrients, they are not suitable for feeding animals directly

Method used

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  • Bacillus amyloliquefaciens culture, preparation method and application thereof

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preparation example Construction

[0043] A preparation method of bacillus amyloliquefaciens culture, is characterized in that, comprises the steps:

[0044] (1) Preparation of solid-state fermentation base material: pretreated white distiller's grains powder is the main fermentation base material, puffed corn flour is used as carbon source, diamine hydrogen phosphate is used as nitrogen source, and calcium carbonate is used as acidity regulator; wherein the added amount of puffed corn flour is 5-15% (w / w pretreated distiller's grain powder); the addition of diammonium phosphate is 5% (w / w pre-treated distiller's grain powder); the addition of calcium carbonate is 1.5-2.0% (w / w pretreatment distiller's grains powder);

[0045] (2) Preparation of Bacillus amyloliquefaciens seed solution: activating Bacillus amyloliquefaciens with a liquid medium, subcultured to make a seed solution;

[0046] (3) Fermentation: Inoculate the seed solution prepared in (2) into the solid-state fermentation base material in (1), the...

Embodiment 1

[0059] Take 5 kg of fresh distiller's grains, add 15% (w / w distiller's grains) puffed corn flour, 0.5% (w / w distiller's grains) diammonium hydrogen phosphate, and replenish water to prepare a fermentation base material. Spread the fermentation base material in the fermentation workshop, with a stacking thickness of 15cm, adjust the temperature of the fermentation workshop, and raise the temperature of the base material to 35±0.2°C. The activated bacillus amyloliquefaciens is diluted and evenly sprayed on the surface of the fermentation base material according to the inoculation amount of 1% (w / v). During the first 12 hours of the fermentation process, the material was turned every 4 hours, and after 12 hours of fermentation, the material was turned every 2 hours. During the fermentation process, samples were taken every 6 hours to determine the pH. The total fermentation time is 72 hours. After the fermentation is over, the number of viable bacteria of Bacillus amyloliquefacie...

Embodiment 2

[0067] The difference between this embodiment and embodiment 1 is only that 8% (w / w distiller's grains) puffed corn flour is added, and the rest of the steps and parameters are the same as in embodiment 1. After the fermentation, the number of live bacteria of Bacillus amyloliquefaciens can reach 18.1 billion / g by dilution coating plate method. The fermented culture is dried, pulverized, and passed through a 100-mesh sieve. Measure and calculate the crude protein content, soluble protein content and the change rate of crude fiber, and include them in Table 1.

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Abstract

The invention discloses a bacillus amyloliquefaciens culture, a preparation method and an application thereof. The number of viable bacteria of cultured species Bacillus amyloliquefaciens is 150-250 billion / g; the bacillus amyloliquefaciens culture is prepared by pretreatment of white wine grain powder as a main fermentation base, expanded corn flour as a carbon source and diammonium hydrogencarbonate as a nitrogen source by solid fermentation; the preparation method of the pretreated white wine grain comprises drying and removing water and alcohol, and removing the rice husk, preparing the rice husk into biomass carbon, mixing with the dried distiller's grains, and preparing the pretreated the white wine grain by puffing treatment. The use of the Bacillus amyloliquefaciens culture of thepresent invention for an animal feed additive can effectively prevent diarrhea and promote the growth rate of animals.

Description

technical field [0001] The invention relates to the technical field of preparation of animal feed additives, in particular to a culture of Bacillus amyloliquefaciens, a preparation method and an application thereof. Background technique [0002] Fresh distiller's grains are the by-products of liquor produced by using grain as the main raw material and using the unique solid-state fermentation and solid-state distillation traditional winemaking process. Since the biological fermentation in the brewing process is mainly to convert the starch in the grain into alcohol, although the nutritional components and the content of the same component in distiller's grains are also different due to the different raw materials and processes used, the components are basically similar. The nutrients in distiller's grains mainly come from bacteria and their metabolic products and bacteria autolysates, except for some raw material residues left by saccharification and incomplete fermentation....

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/30A23K50/10A23K10/12A23K10/38A23K10/30A23K20/26A23K20/20C12R1/07
CPCA23K10/12A23K10/30A23K10/38A23K20/20A23K20/26A23K50/10A23K50/30C12N1/20Y02P60/87
Inventor 彭楠刘云常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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