Preparation method of PVA-SA composite immobilized carrier for degrading microorganisms

A PVA-SA, immobilized carrier technology, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problem of easy inactivation of activated sludge, low pollutant concentration, Problems such as poor degradation efficiency, to achieve the effect of being conducive to growth and reproduction, good mass transfer performance, and safe production process

Active Publication Date: 2019-11-15
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the additive, synergistic, and antagonistic effects among various pollutants, when conventional biological methods are used for wastewater treatment, the coking wastewater is more toxic to microbial growth, resulting in coking in biological treatment. In the case of wastewater, there are many problems such as low concentration of tolerated pollutants, poor degradation efficiency, and easy inactivation of activated sludge, which seriously affects the application of the measure of using biological methods to treat complex polluted coking wastewater.

Method used

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  • Preparation method of PVA-SA composite immobilized carrier for degrading microorganisms
  • Preparation method of PVA-SA composite immobilized carrier for degrading microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the method for screening phenol, pyridine, quinoline efficient degrading flora

[0027] The method comprises the steps of:

[0028] 1) Sampling

[0029] The coking wastewater with sludge was collected from the sewage treatment plant of Tangshan Dafeng Coking Co., Ltd., including 1mL raw water (with mud), 1mL anaerobic pool effluent (with mud), 1mL aerobic pool (with mud), 1mL distillate Water (with mud) comes out of the settling tank. A

[0030] 2) Cultivation and transfer

[0031] The coking waste water collected at each process point collected in step 1) is added to 100mL liquid inorganic salt medium together, and the proportion of said liquid inorganic salt medium is NaCl 1.0g / L, NH 4 NO 3 1.0g / L, K 2 HPO 4 1.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O 0.2g / L, phenol 50mg / L, pyridine 50mg / L, quinoline 50mg / L. Put it in the mouth of the triangular flask and seal it with a parafilm (the surface of the parafilm has air holes), and then place it in ...

Embodiment 2～5

[0035] Examples 2-5: A preparation method of a PVA-SA composite immobilized carrier embedding degrading microorganisms.

[0036] Preparation of degrading bacterial group seed solution: transfer the enriched bacterial solution obtained in Example 1 into LB liquid medium, and cultivate at 30° C. and 180 rpm for 48 hours to obtain seed solution.

[0037] Preparation of bacterial suspension: Inoculate 1 mL of the above seed solution into 100 mL of LB medium, incubate at 180 r / min at 30 °C for 24 h, centrifuge at 6000 r / min at 4 °C for 10 min, discard the supernatant, and resuspend by blowing with sterile water , centrifugal, abandon supernatant, repeat like this 3 times, the thallus that obtains is washed with inorganic salt solution (the inorganic salt content of described inorganic salt solution is NaCl 1.0g / L, NH 4 NO 3 1.0g / L, K 2 HPO 4 1.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O 0.2g / L, 1mL trace element stock solution / L, the composition of described trace element stoc...

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Abstract

The invention relates to a preparation method of a PVA-SA composite immobilized carrier for degrading microorganisms. The method is characterized in that the method comprises the following steps: a) mixing a bacterial suspension of degrading microorganisms and a sterilized composite material solution under aseptic conditions; wherein the composite material solution is composed of 95 to 105 g/L ofpolyvinyl alcohol, 9 to 11 g/L of sodium alginate, 28 to 32 g/L of SiO2, 4.5 to 5.5 g/L of CaCO3, 5 to 7 g/L of activated carbon and balance of deionized water; and b) in the sterile environment, dropping the composite material solution homogeneously mixed with the bacterial suspension obtained in step 1) into a cross-linking agent, and after hardening treatment, obtaining the immobilized carrier,wherein the cross-linking agent is obtained by dissolving calcium chloride solid with a mass fraction of 1.8 to 2.2% in a saturated boric acid solution and adjusting the pH to 6.1 to 6.3 with a sodium hydroxide solution.

Description

technical field [0001] The invention relates to a microbial immobilized carrier for biological treatment of coking wastewater and a preparation method thereof. Background technique [0002] Coking wastewater is wastewater generated during coke refining, gas purification and coking recovery. It contains many complex organic pollutants, including quinolines, pyridines, phenols, benzene and other organic substances, and is listed as refractory sewage. . Due to the additive, synergistic, and antagonistic effects among various pollutants, when conventional biological methods are used for wastewater treatment, the coking wastewater is more toxic to microbial growth, resulting in coking in biological treatment. In the case of wastewater, there are many problems such as low concentration of tolerated pollutants, poor degradation efficiency, and easy inactivation of activated sludge, which seriously affects the application of the measure of using biological methods to treat complex ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C12N11/14C12N11/04C12R1/01
CPCC12N11/04C12N11/08C12N11/10C12N11/14
Inventor 杨宗政王菊吴志国许文帅赵晓宇吴芮
Owner TIANJIN UNIV OF SCI & TECH
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