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Xinjiang berberis heteropoda extract and preparation method and application thereof

A technology of barberry black fruit, extract, applied in the field of medicine, can solve problems such as difficulty in relieving symptoms

Inactive Publication Date: 2019-11-19
XINJIANG MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Western medicines currently used to treat diarrhea-predominant IBS include antispasmodics, anticholinergics, antidiarrheals, visceral analgesics, antipsychotics, probiotics, etc., but they all target a specific pathological link Intervention, difficulty relieving overall symptoms

Method used

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  • Xinjiang berberis heteropoda extract and preparation method and application thereof
  • Xinjiang berberis heteropoda extract and preparation method and application thereof
  • Xinjiang berberis heteropoda extract and preparation method and application thereof

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preparation example Construction

[0024] The invention provides the preparation method of the Berberis sativa extract, which comprises the following steps: mixing the Berberis sativa root powder and water, extracting the obtained suspension for 2-3 hours, separating the solid and liquid, and collecting the liquid phase to obtain Xinjiang black barberry extract; the solvent includes 0.4% sulfuric acid aqueous solution, distilled water, 95% ethanol aqueous solution or methanol.

[0025] The present invention mixes Xinjiang black barberry root powder with a solvent to obtain a suspension. The volume ratio of the mass of the Berberry root powder and the solvent is preferably 4-6g:50mL, more preferably 5g:50mL. The particle size of the root powder of Berberis sinensis is preferably 40-80 mesh, more preferably 70 mesh. The present invention has no special limitation on the source of the root of Berberis nigrum, and the root of Berberis nigrum well-known in the art can be used. In the embodiment of the present inve...

Embodiment 1

[0042] 1 solution preparation

[0043] 1.1 Preparation of sample solution

[0044] Take by weighing 8 parts of Berberis nigra root powder, every part of 5g, into a 50mL Erlenmeyer flask, add 0.4% sulfuric acid solution, distilled water, 95% ethanol, methyl alcohol of 10 times the amount respectively, by decoction method (80 ℃ decoction Twice, 1h / time) extract Berberis nigrumensis root, filter, combine the filtrates, evaporate the solvent to obtain extracts of different solvent extracts, add methanol to dissolve, accurately measure 2 mL of the filtrate, place it in a 10 mL measuring bottle, Dilute to volume with methanol, shake well to obtain a sample solution with a certain concentration, filter through a 0.22 μm filter membrane to remove impurities, store in a refrigerator at 4°C, and set aside.

[0045] 1.2 Preparation of mixed reference substance

[0046] Accurately weigh 1.28mg, 0.54mg, 1.03mg, 1.06mg, 2.97mg, 1.28mg, 0.54mg, 1.03mg, 2.97mg mg, 1.99mg and 2.32mg, placed...

Embodiment 2

[0056] Weigh 5 g of Berberis nigrumen root powder, add 10 times the amount of distilled water respectively, extract Berberis nigrumensis root by ultrasonic extraction (50°C extraction twice, 1h / time), filter, collect the filtrate, and remove the solvent , to obtain the extract extract. Accurately weigh a certain amount of extract extract (see Table 2 for the amount of sample to be weighed), add water to dissolve and set the volume to 5 mL, and obtain a sample solution (see Table 2 for the concentration).

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Abstract

The invention provides a Xinjiang berberis heteropoda extract and a preparation method and application thereof, and belongs to the technical field of medicines. The Xinjiang berberis heteropoda extract comprises magnoflorine, berbamine, a ferulic acid, columbamine, jatrorrhizine, palmatine and berberine. The application of the Xinjiang berberis heteropoda extract to inhibiting the growth of enteropathogenic bacteria, destroying the permeability of a cell wall, integrity of a cell membrane structure and / or changing the enzyme concentration of ATP in the enteropathogenic bacteria is achieved; and the enteropathogenic bacteria includes staphylococcus aureus, escherichia coli or pseudomonas aeruginosa. The application of the Xinjiang berberis heteropoda extract in preparation of drugs for thetreatment of IBS is achieved, and at the same time, the Xinjiang berberis heteropoda extract participates in brain gut movement to relieve diarrhea, abdominal pain and depression-like symptoms in IBSrats. The Xinjiang berberis heteropoda extract can alleviate abnormal pathological changes such as gap widening around the blood vessel, mild edema and blood stasis in the brain tissue of the IBS rats.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to an extract of Berberis sativa from Xinjiang and its preparation method and application. Background technique [0002] Berberis heteropoda Schrenk is a deciduous shrub of Berberidaceae (Berberidaceae) Berberis genus (Berberidaceae.L). The root can be used to treat acute gastroenteritis, dysentery, urinary system infection and other diseases. However, in the prior art, there is no public report on the types of active ingredients that play a role in treating diseases in Xinjiang black fruit barberry. [0003] Irritable bowel syndrome (IBS) is a chronic, recurrent gastrointestinal dysfunction disease. Its clinical manifestations are mainly characterized by abdominal pain, diarrhea and (or) constipation, often accompanied by mental and psychological symptoms. obstacle. The etiology and pathogenesis of IBS are not yet fully understood, and it may be related to various ...

Claims

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Application Information

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IPC IPC(8): A61K36/29A61P1/00A61P1/12A61P31/04A61K31/4748A61K31/192A61K31/4375A61K31/473
CPCA61K31/192A61K31/4375A61K31/473A61K31/4748A61K36/29A61P1/00A61P1/12A61P31/04A61K2300/00Y02A50/30
Inventor 李莉李飞朱慧敏闫琦冯敏
Owner XINJIANG MEDICAL UNIV
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