A method for improving tartary buckwheat seedling resistance to heavy metal lead stress

A technology of anti-heavy metals and seedlings, applied in the direction of seed and rhizome treatment, application, grain cultivation, etc., to achieve the effect of increasing germination rate, promoting transformation and reuse, and increasing the activity of POD and CAT

Active Publication Date: 2021-08-24
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main methods to alleviate heavy metal stress damage to plants include mineral elements, plant growth regulators, microorganisms, etc. Some mineral factors can alleviate specific heavy metal stress, such as calcium and silicon can alleviate the damage of heavy metal aluminum stress to plants, and ion Whether titanium can alleviate the damage caused by lead stress in tartary buckwheat remains unknown

Method used

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  • A method for improving tartary buckwheat seedling resistance to heavy metal lead stress
  • A method for improving tartary buckwheat seedling resistance to heavy metal lead stress
  • A method for improving tartary buckwheat seedling resistance to heavy metal lead stress

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Embodiment 1

[0033] A method for improving tartary buckwheat seedling resistance to heavy metal lead stress, comprising the following steps:

[0034] (1) Choose tartary buckwheat seeds with no scars on the outside and full grains, dry them in the sun for 2 days, every day for 5-6 hours, then use a concentration of 0.1wt% potassium permanganate solution to disinfect them for 2 hours, and rinse them with distilled water for 3 times;

[0035] (2) Submerge the washed seeds in step (1) in the seed soaking solution, and soak the seeds at a constant temperature of 25°C for 10 hours; wherein, the seed soaking solution includes ionic titanium, sodium hydrosulfide, γ-aminobutyric acid and molybdenum Ammonium acid, the concentration of ionic titanium is 0.02mmol / L, the concentration of sodium hydrosulfide is 3mmol / L, the concentration of γ-aminobutyric acid is 0.3mmol / L, and the concentration of ammonium molybdate is 0.5mmol / L;

[0036] (3) Wipe and sterilize the seed germination box with alcohol, th...

Embodiment 2

[0039] A method for improving tartary buckwheat seedling resistance to heavy metal lead stress, comprising the following steps:

[0040] (1) Choose tartary buckwheat seeds with no scars on the outside and full grains, dry them in the sun for 3 days, every day for 5-6 hours, then use a concentration of 0.1wt% formaldehyde solution to disinfect them for 2 hours, then rinse them with distilled water for 4 times;

[0041] (2) Submerge the washed seeds in step (1) in the seed soaking solution, and soak the seeds at a constant temperature of 25°C for 12 hours; wherein, the seed soaking solution includes ionic titanium, sodium hydrosulfide, γ-aminobutyric acid and molybdenum Ammonium acid, the concentration of ionic titanium is 0.05mmol / L, the concentration of sodium hydrosulfide is 12mmol / L, the concentration of γ-aminobutyric acid is 0.6mmol / L, and the concentration of ammonium molybdate is 0.7mmol / L;

[0042](3) Wipe and sterilize the seed germination box with alcohol, then put it...

Embodiment 3

[0045] A method for improving tartary buckwheat seedling resistance to heavy metal lead stress, comprising the following steps:

[0046] (1) Choose tartary buckwheat seeds with no scars on the outside and full grains, dry them in the sun for 3 days, every 6 hours, then disinfect them with 0.1 wt% hydrogen peroxide solution for 2 hours, and rinse them with distilled water for 5 times;

[0047] (2) Submerge the washed seeds in step (1) in the soaking solution, and soak the seeds at a constant temperature of 25°C for 24 hours; wherein, the soaking solution includes ionic titanium, sodium hydrosulfide, γ-aminobutyric acid and molybdenum Ammonium acid, the concentration of ionic titanium is 0.08mmol / L, the concentration of sodium hydrosulfide is 24mmol / L, the concentration of γ-aminobutyric acid is 0.9mmol / L, and the concentration of ammonium molybdate is 0.8mmol / L;

[0048] (3) Wipe and sterilize the seed germination box with alcohol, then put it into double-layer filter paper, an...

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Abstract

The invention provides a method for improving the ability of tartary buckwheat seedlings to resist heavy metal lead stress, comprising the following steps: selecting tartary buckwheat seeds with no scars on the outside and full grains, drying them in the sun, disinfecting them with disinfectant, and washing them with distilled water; Submerge the washed seeds in the seed soaking solution and soak them at a constant temperature; wipe and disinfect the seed germination box with alcohol, then put it into double-layer filter paper, spread the soaked seeds evenly on the filter paper, and cover the box Cover and place in a shading incubator to obtain tartary buckwheat seedlings; select strong seedlings that grow neatly, plant them in loose and flat soil, cover the roots with soil, and then apply mixed fertilizer to each seedling. The invention cultivates the seedlings by means of disinfection, seed soaking and shading cultivation, and can effectively improve the vitality of the tartary buckwheat seedlings under the lead stress condition, so as to resist the heavy metal lead stress and increase the germination rate of the tartary buckwheat seeds.

Description

technical field [0001] The invention belongs to the technical field of agricultural production, and in particular relates to a method for improving the ability of tartary buckwheat seedlings to resist heavy metal lead stress. Background technique [0002] Tartary buckwheat has high nutritional value and medicinal value, strong stress resistance, and short growth cycle. Tartary buckwheat is widely used, not only as food, health food and medicine, but also as an important industrial raw material. During the growth of tartary buckwheat, a large amount of rutin will be produced, which is used in the production of health medicine or tea for the prevention and treatment of chronic diseases such as diabetes, hypertension, and obesity, with extremely high added value. Tartary buckwheat is in great demand in today’s market, and resources are scarce. Countries compete to buy from my country. Japan alone needs to import hundreds of thousands of tons every year. my country can only mee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/20A01C1/00C05G1/00
CPCA01C1/00A01G22/20C05B1/02C05C3/00C05C11/00C05D9/00C05D9/02C05C9/00C05D1/00C05F11/00
Inventor 万燕欧阳建勇叶巧玲乐梨庆马成瑞向达兵马前瑞盛正祥张加志赵钢邬晓勇吴琪
Owner CHENGDU UNIV
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