Preparation method of triiron tetroxide / titanium dioxide / silver core shell nano-material and recyclable immunoassay application thereof

A core-shell nanomaterial, ferric tetroxide technology, applied in the direction of iron oxide/iron hydroxide, titanium dioxide, titanium oxide/hydroxide, etc., can solve the problems of high cost, unfavorable practical application, etc., to achieve enhanced efficiency, sample Simple and fast cleaning and separation

Active Publication Date: 2019-12-03
浙江高美基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the materials based on surface-enhanced Raman scattering technology are mostly noble metal nanomaterials, which are expensive to prepare, which is not conducive to their practical application in clinical immunoassays.

Method used

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  • Preparation method of triiron tetroxide / titanium dioxide / silver core shell nano-material and recyclable immunoassay application thereof
  • Preparation method of triiron tetroxide / titanium dioxide / silver core shell nano-material and recyclable immunoassay application thereof
  • Preparation method of triiron tetroxide / titanium dioxide / silver core shell nano-material and recyclable immunoassay application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] a kind of Fe 3 o 4 / TiO 2 / Ag triple core-shell nanomaterial and its direct recyclable immunoassay method for prostate-specific antigen PSA, comprising the following steps:

[0031] 1. Fe 3 o 4 / TiO 2 Preparation of / Ag triple core-shell nanomaterials

[0032] (1) Dissolve 3.5 grams of ferric chloride hexahydrate, 10.8 grams of sodium acetate and 3 grams of polyethylene glycol in 120 milliliters of ethylene glycol, sonicate until completely dissolved, transfer to a hydrothermal reaction kettle, and heat at 200 ° C The reaction was carried out for 10 hours, centrifuged and washed with ethanol, and the precipitate was collected to obtain Fe 3 o 4 nanoparticles;

[0033] (2) Weigh 50 mg of Fe obtained in step (1) 3 o 4 Nanoparticles were dissolved in hydrochloric acid aqueous solution with a concentration of 0.1 mmol per milliliter and sonicated for 15 minutes, magnetically separated and washed with deionized water three times; after magnetic separation again, th...

Embodiment 2

[0050] With above-mentioned embodiment 1, its difference is: Fe 3 o 4 / TiO 2 In the preparation of Ag core-shell nanomaterials: in step (1), 1.35 grams of ferric chloride hexahydrate, 3.6 grams of sodium acetate and 1.0 grams of polyethylene glycol were dissolved in 40 ml of ethylene glycol, and ultrasonicated until completely dissolved , transferred to a hydrothermal reactor, reacted at 200°C for 10 hours, centrifuged and washed with ethanol, and the precipitate was collected to obtain Fe 3 o 4 Nanoparticles; the volume of tetrabutyl titanate added in step (2) was 1 ml.

[0051] Figure 10 The frequency shift in the Raman spectrum is 1264 cm -1 The variation diagram of the characteristic peak intensity of Raman with the concentration of the antigen to be tested can be seen through fitting. When the concentration of the antigen to be tested is changed from 100 pg / ml to 0.1 mg / ml, the Raman characteristic peak intensity changes linearly with the concentration. The fitting...

Embodiment 3

[0053] With above-mentioned embodiment 1, its difference is: Fe 3 o 4 / TiO 2 In the preparation of / Ag core-shell nanomaterials: in step (1), 6.75 grams of ferric chloride hexahydrate, 18.0 grams of sodium acetate and 5.0 grams of polyethylene glycol were dissolved in 200 ml of ethylene glycol, and ultrasonicated until completely dissolved , transferred to a hydrothermal reactor, reacted at 200°C for 10 hours, centrifuged and washed with ethanol, and the precipitate was collected to obtain Fe 3 o 4 Nanoparticles; the volume of tetrabutyl titanate added in step (2) was 6 ml.

[0054] Figure 11 The frequency shift in the Raman spectrum is 1264 cm -1 The variation diagram of the characteristic peak intensity of Raman with the concentration of the antigen to be tested can be seen through fitting. When the concentration of the antigen to be tested is changed from 100 pg / ml to 0.1 mg / ml, the Raman characteristic peak intensity changes linearly with the concentration. The fitt...

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Abstract

The invention discloses a preparation method of triiron tetroxide / titanium dioxide / silver core shell nano-material and direct recyclable immunoassay application thereof. The application is characterized by activating the triple core shell nano-material to enable the same to be combined with to-be-detected antigens, using a surface-enhanced Raman scattering effect to realize direct Raman detection of the to-be-detected antigens, carrying out ultraviolet-illumination antigen degradation on the above-mentioned immunity-combined nano-material after Raman detection is completed, magnetically recovering the Fe<3>O<4> / TiO<2> / Ag core shell nano-material of which a surface has no antigen existing, using the same on next detection on to-be-detected antigens, and repeating the Raman detection-catalytic degradation-Raman detection process to realize direct recyclable immunoassay of tumor markers. The method has the advantages that a three-layer core shell structure has functions of magnetic separation and Raman enhancement, also has a function of catalytic degradation on the tumor markers, a detection limit is low at the same time, and macro-molecular tumor markers can be detected.

Description

technical field [0001] The invention relates to the fields of material engineering and nanotechnology, in particular to a ferric oxide / titanium dioxide / silver (Fe 3 o 4 / TiO 2 / Ag) core-shell nanomaterials and their recyclable immunoassay applications. Background technique [0002] In recent years, with the aggravation of environmental pollution, the continuous change of global climate, the emergence of food crisis and the increase of people's living pressure, cancer occurs frequently, and its high mortality rate has become a serious threat to human health. Therefore, people have developed a series of advanced immunological techniques, such as biochemical detection techniques such as chemiluminescence and enzyme-linked immunoassay, to detect and treat cancer patients. However, for patients in the early stages of cancer, the levels of cancer markers in their blood are low, making it difficult to detect and treat them early. The surface-enhanced Raman scattering immunotech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/543G01N33/537G01N21/65C01G49/08C01G23/053
CPCG01N33/532G01N33/54326G01N33/537G01N21/65C01G49/08C01G23/053C01P2004/04C01P2004/03
Inventor 姜涛周骏姜勇杜远远顾辰杰王福艳
Owner 浙江高美基因科技有限公司
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