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Heterotrophic ammonia oxidizing bacteria and application thereof

A technology of ammonia oxidizing bacteria and strains, applied in the field of biological treatment of environmental pollutants, can solve problems such as no research reports

Active Publication Date: 2019-12-20
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Heterotrophic nitrification and heterotrophic nitrification microorganisms were actually reported as early as 1886, 1894 and 1908, but due to strong criticism and negation by Winogradsky (Winogradsky), there were almost no reports in the following years. research report [3]

Method used

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  • Heterotrophic ammonia oxidizing bacteria and application thereof
  • Heterotrophic ammonia oxidizing bacteria and application thereof
  • Heterotrophic ammonia oxidizing bacteria and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1: Isolation and identification of Halomonas venusta MA-ZP17-13

[0046] Collect seawater samples from a Penaeus vannamei culture pond in Zhangpu County, Zhangzhou City, Fujian Province, take 2mL of seawater samples collected, and transfer them to sterilized LB (peptone 5g / L, yeast extract 5g / L, sodium chloride (NaCl) 10g / L, pH value 7.4~8.2) enrich culture in the Erlenmeyer flask of enriched culture solution, one week is a cycle, after each cycle, take an appropriate amount of culture solution and centrifuge, discard the supernatant, collect the precipitate, and obtain the bacteria , and then wash the thalline 3 times with physiological saline under aseptic conditions, transfer to fresh sterilized heterotrophic nitrification medium with 2% inoculum size to continue culturing, repeat the cycle, and carry out domestication. Take 2 mL of enrichment medium and transfer it to sterilized selection medium (ammonium chloride (NH 4 Cl) 0.382g / L, sodium acetate (CH 3 ...

Embodiment 2

[0048] Example 2: Performance of Halomonas venusta MA-ZP17-13 Ammonia Nitrogen Removal and Heterotrophic Ammonium Oxidation

[0049] Inoculate Halomonas venusta MA-ZP17-13 with ammonium chloride (NH 4 In the heterotrophic nitrification medium with Cl) as the only nitrogen source, oxygen is introduced to ensure aerobic respiration, and acetylene gas is injected at the same time to inhibit denitrification. Cultivate in a shaker at 140r / min at 10°C, take samples at intervals and detect changes in the concentrations of ammonia nitrogen, nitrate and nitrite. Depend on figure 2 It can be seen that the ammonia nitrogen removal rate of Halomonas venusta MA-ZP17-13 after 3 days of cultivation can reach 88.8%. Depend on image 3 It can be seen that the heterotrophic nitrification rate of Halomonas venusta MA-ZP17-13 was 0.0166mg / L after 3 days of culture -1 .h.

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Abstract

The invention discloses heterotrophic ammonia oxidizing bacteria and application thereof and relates to the technical field of biological treatment of environmental pollutants. The heterotrophic ammonia oxidizing bacteria come from penaeus vanmamei culture seawater and are obtained after artificial enrichment culture, separation and purification, which are strain MA-ZP17-13 gram-negative Halomonavenusta. Sequencing of genome of the strain MA-ZP17-13 gram-negative Halomona venusta finds that the strain contains an ammonia nitrogen oxygenase gene. The biological characteristics of the heterotrophic ammonia oxidizing bacteria are catalase and oxidase positive, obligate aerobic, rod-like strain form, milk white and opaque colonies, regular edges, surface bulge, no aureole and diameter of 1-2mm. The heterotrophic ammonia oxidizing bacteria can be applied to removal of ammonia nitrogen from salt-containing sewage.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a strain of heterotrophic ammonia oxidizing bacteria and its application. Background technique [0002] Since the 1990s, the aquaculture industry in my country's coastal waters has developed rapidly. Although the intensive and high-density aquaculture model has brought high aquaculture output, it has brought a large amount of residual feed and excreta to the aquaculture water body, resulting in water bodies The eutrophication and even the outbreak of Algal Blooms have caused serious damage to the ecological balance of coastal waters. [0003] Nitrogen-containing compounds are essential substances in the process of biological metabolism and play an extremely important role in the life cycle [1] . However, some nitrogen-containing compounds are not favored, for example, ammoniacal nitrogen. Ammonia nitrogen can not only cause water quality...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20
CPCC02F3/34C02F2101/16C02F2103/20C12N1/205C12R2001/01
Inventor 邵宗泽李贵珍赖其良
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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