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A kind of marine bacterium degrading vomitoxin and its application

A vomitoxin and marine technology, applied in the field of microorganisms, can solve the problems of polluting animal and human health and harm, and achieve the effects of mild effect, high detoxification activity and simple operation.

Active Publication Date: 2021-04-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to screen out new strains capable of degrading vomitoxin from a large number of natural samples, thereby solving the problem that vomitoxin contamination in feed and food is harmful to animals and human health

Method used

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  • A kind of marine bacterium degrading vomitoxin and its application
  • A kind of marine bacterium degrading vomitoxin and its application
  • A kind of marine bacterium degrading vomitoxin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Screening and Identification of Example 1 Bacterial Strains

[0050] The specific steps of strain screening are as follows:

[0051] 1) Screening of strains: In this study, strains were screened from marine soil samples.

[0052] Seed solution preparation: Weigh 1g of marine soil into a sterile test tube, add 9mL of sterile saline, shake overnight at 28°C and 140r / min on a shaker; after standing for 1h, draw 100μL of supernatant, and incubate with 900μL of 2216E liquid base mixture, and cultured in a 28°C incubator with shaking for 12 hours to obtain a seed liquid.

[0053] Among them, the formula of 2216E liquid medium is: peptone 5.0g / L, yeast extract powder 1.0g / L, ferric citrate 0.1g / L, sodium chloride 19.45g / L, magnesium chloride 5.98g / L, sodium sulfate 3.24g / L, calcium chloride 1.8g / L, potassium chloride 0.55g / L, sodium carbonate 0.16g / L, potassium bromide 0.08g / L, strontium chloride 0.034g / L, boric acid 0.022g / L, silicic acid Sodium 0.004g / L, calcium fluoride...

Embodiment 2

[0060] The culture method of embodiment 2 marine bacilli of the present invention

[0061] Take Pelagibacterium halotolerans ANSP101CGMCC No.17304

[0062] 2.0mL (the concentration of live bacteria is 10 9 CFU / mL), inoculated in 100mL medium for shake flask fermentation culture, the fermentation temperature was 28°C, the pH value was 7.6, the rotational speed was 200r / min, and the fermentation time was 24h.

[0063] Among them, the shake flask fermentation medium is composed of the following components: peptone 5.0g, yeast extract powder 1.0g, sodium chloride 19.45g, magnesium chloride 5.98g, sodium sulfate 3.24g, calcium chloride 1.8g, potassium chloride 0.55g , sodium carbonate 0.16g, distilled water 1000mL, pH 7.6.

[0064] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 3

[0065] Embodiment 3 Salt-tolerant characteristic research of marine bacterium of the present invention

[0066] Take Pelagibacterium halotolerans ANSP101CGMCC No.17304

[0067] 2.0mL (the concentration of live bacteria is 10 9 CFU / mL), respectively inoculated in 100mL fermentation medium containing 0%, 0.5%, 1%, 4%, 8% and 12% NaCl for cultivation, the fermentation temperature was 28°C, the pH value was 7.6, and the rotation speed was 200r / min , the fermentation time was 24h, and the OD was measured every 3h after inoculation 600 , draw the growth curve of ANSP101 under different NaCl concentrations.

[0068] Determination results: Marine bacteria ANSP101 can grow normally in the medium with NaCl concentration less than 4%; 8% NaCl has a certain inhibitory effect on it, and the growth is slow; 12% NaCl completely inhibits the growth of the bacteria ( image 3 ).

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Abstract

The invention relates to a marine bacterium Pelagibacterium halotolerans ANSP101 CGMCC No. 17304 for degrading vomitoxin, a cultivation method and application thereof. Through the method, the degradation rate of the marine bacterium fermented liquid to the vomitoxin can reach 79%; the degradation rate of the active protein produced by the marine bacterium to the vomitoxin can reach 80.21%. The strain or the active protein produced by the strain and the prepared additives can be used for complete feed, concentrated feed, premixed feed for pigs, chickens, cattle, sheep, pets, aquatic animals, fur animals, grains, corn, peanut meal, Wheat, oats and other feed materials, silage, grain, grain oil, grain processing and agricultural by-products, aged grain, industrial ethanol processing by-product DDGS, biological detoxification of vomitoxin in food, tea and Chinese herbal medicine.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of Pelagibacterium halotolerans capable of degrading vomitoxin and its active protein. Background technique [0002] Deoxynivalenol (DON) was first discovered in 1970 in sick wheat infected with head blight in Kagawa Prefecture, Japan. Because its structure is a 4-deoxy derivative of nivalenol, it was named deoxynivalenol (Deoxynivalenol, DON). DON is mainly produced by Fusarium graminearum and Fusarium chrysogenum, etc. It belongs to Class B trichothecene toxins, and its pollution to cereal crops ranks first among Fusarium toxins, seriously threatening the health of humans and livestock. Contamination of food and feed samples with DON is common worldwide. The World Health Organization's 2001 report showed that the detection rate of vomitoxin in oats was as high as 68%, and that in other grains also reached 27% to 59%. The European U...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C07K1/14A23K10/18A23K10/16A23K20/147A23K20/28A23K20/20A23K10/30A23K10/37A23K20/163A23K10/20A23K30/18A23K50/10A23K50/75A23K50/30A23K50/80A23K50/40A23L29/30A23L29/00C12R1/01
CPCC12N1/20C07K1/145A23K10/18A23K10/16A23K20/147A23K20/28A23K20/20A23K10/30A23K10/37A23K20/163A23K10/20A23K30/18A23K50/10A23K50/75A23K50/30A23K50/80A23K50/40A23L29/065A23L29/03A23L29/30A23L29/015A23L29/00C12R2001/01C12N1/205A23V2400/125A23V2400/137A23V2400/113A23V2400/143A23V2400/173A23V2400/169A23V2400/249A23V2400/513A23V2400/529A23V2400/515A23V2400/517A23V2400/519A23V2400/533Y02A40/818Y02P60/87
Inventor 赵丽红计成马秋刚张静郭永鹏
Owner CHINA AGRI UNIV
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