Slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation

An electrode structure and slide rail technology, applied in the field of cell sorting, can solve the problems of controlling cell trajectory, complex silicon-based electrode processing technology, and low work efficiency, so as to improve work efficiency, realize three-dimensional control and separation, and improve separation The effect of purity

Active Publication Date: 2019-12-31
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the reported electrode structures for dielectrophoretic cell separation use planar electrode structures or three-dimensional electrode structures with uniform side walls, such as Chinese patent 201510585845.6 and Chinese patent 201510379158.9. These types of electrodes cannot generate effective electric fields along the depth of the channel. However, the three-microelectrode structure with non-uniform side walls, which can generate effective electric field gradients in the depth direction of the channel, can only achieve batch-type cell separation at low flow rates due to structural limitations. Low, such as Chinese patent 201811550218.9
The Yobas research group of the Hong Kong Univ

Method used

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  • Slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation
  • Slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation
  • Slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation

Examples

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Embodiment 1

[0072] In this example, the microchip prepared by the slide-rail electrode structure obtained in the above embodiment and live / dead HeLa cells were used to test the effect of cell separation. The specific steps are as follows:

[0073] Extract the adherent HeLa cells and suspend them in the cell culture medium, take out about 1.5ml of the cell suspension and place it in a small centrifuge tube with a volume of 1.5ml, and treat it in a water bath environment at 65°C for 15 minutes. To kill the cells, centrifuge the treated cells and replace the solution with a 300 mM D-mannitol solution, then use propidium iodide (PI) to stain the dead cells for two minutes; add calcein to the remaining live cell suspension to Live cells were stained for 10 min. The live / dead cells after staining are mixed in the 300mM D-mannitol solution with a conductivity of 0.01S / m according to the concentration ratio of live cells: dead cells = 10:1, and the concentration of live cells in the resulting mix...

Embodiment 2

[0076] In this example, the microfluidic chip prepared by the V-shaped slide rail electrode structure obtained in the above embodiment and the mixed solution of HeLa cells and polystyrene microspheres were used to test the effect of particle sorting. The specific steps are as follows:

[0077] Extract adherent HeLa cells and suspend them in cell culture medium, add calcein for 10 minutes of staining, then centrifuge the cell suspension and replace the solution with 300mM D-mannitol with a conductivity of 0.01S / m solution. After centrifugation, 7 μm microspheres were added to the cell solution to obtain a cell concentration of 1.2×10 7 A / ml, 7μm microsphere concentration is 1×10 6 A / ml mixed solution. The solution is injected into the above-mentioned microfluidic chip from the middle sample inlet 18 at a flow rate of 1.2ml / h, and the buffer solution (0.01S / m 300mM D-mannitol solution). A sinusoidal signal with an amplitude of 35Vpp and a frequency of 400kHz is input from th...

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Abstract

The invention discloses a slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation and belongs to the technical field of micro-fluidic chips. The structure comprises an interdigital electrode array formed by slide rail type electrodes which form an inclination angle alpha with a main runner and cross the main runner, a pair of main electrode which alternately extend to be interdigital and located on two sides of the main runner, parallel sub-runners formed by dividing the main runner by non-uniform side wall structures of the interdigital electrodes, an insulating groove and an inlet/outlet of a sample. The slide rail electrodes with non-uniform side wall structures are placed in the main runner at a certain inclination angle, so that cellsslide along two side walls of a slide layer under the action of dielectrophoresis force and drag force of fluid and enter a migration outlet, and continuous separation of cells is realized. Besides, by changing one-way slide rail type electrodes into two-way V-shaped slide rail type electrodes, the throughput is further improved while continuous cell separation is realized.

Description

technical field [0001] The invention belongs to the technical field of cell sorting in microfluidic chip technology, and in particular relates to a slide rail type dielectrophoresis electrode structure for high-throughput continuous flow cell separation. Background technique [0002] Cell separation technology refers to the technology of obtaining a certain type of cell from a sample containing a variety of cells, which is widely used in many fields of biomedical research and clinical treatment. For example, during water quality testing, bacteria and parasites in water quality can be separated and detected; clinically, the screening and analysis of disease-related cells can be used for disease diagnosis and pathological research, and some rare cells such as circulating tumor cells (CTCs), Screening of circulating fetal cells (CFCs) can provide valuable information for early diagnosis and precise treatment of diseases. [0003] Traditional cell separation methods (such as ce...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/42
CPCC12M23/16C12M47/04
Inventor 邢晓星聂小凤梁自恒俞度立
Owner BEIJING UNIV OF CHEM TECH
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