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Multifunctional lactobacillus casei CCFM1052 for relieving PFOA toxic effect, and fermented food and application of multifunctional lactobacillus casei CCFM1052

A technology of CCFM1052 and Lactobacillus casei, which is applied in the field of microorganisms, can solve the problems of high price and difficulty in obtaining, and achieve the effects of improving metabolic disorders, increasing the content, and improving intestinal flora disorders

Active Publication Date: 2020-01-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these natural substances are expensive and difficult to obtain. In addition, due to the tolerance of the human body, it is not yet known whether a large amount of intake will cause potential harm to the human body.

Method used

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  • Multifunctional lactobacillus casei CCFM1052 for relieving PFOA toxic effect, and fermented food and application of multifunctional lactobacillus casei CCFM1052
  • Multifunctional lactobacillus casei CCFM1052 for relieving PFOA toxic effect, and fermented food and application of multifunctional lactobacillus casei CCFM1052
  • Multifunctional lactobacillus casei CCFM1052 for relieving PFOA toxic effect, and fermented food and application of multifunctional lactobacillus casei CCFM1052

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: Good PFOA adsorption capacity in vitro;

[0073] Bacterial adsorption Lactobacillus casei CCFM1052 was purified and activated for culture, inoculated in MRS liquid medium at 1% (v / v) inoculum, and cultured at 37° C. for 18 hours. Then centrifuge at 8000r / min for 5min to collect the bacteria, take the precipitate and clean it with normal saline, then continue to centrifuge at 8000r / min for 5min to remove the precipitate to obtain live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 10mg / LPFOA solution, and the final bacterial concentration reached 1g dry bacteria / L (the wet bacteria were resuspended in PFOA-free ultrapure water as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOA solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), and its ionic strength has negligible influence on PFOA adsorption. Subsequently, the 250ml Erlenmeyer flask containing 100ml of...

Embodiment 2

[0078] Example 2: Lactobacillus casei CCFM1052 can significantly improve spleen atrophy in mice exposed to PFOA;

[0079] Fifty 6-week-old male C57BL / 6J mice were randomly divided into five groups according to body weight after one week of acclimatization: control group, model group, quercetin intervention group, Lactobacillus casei 15-7 intervention group, LGG intervention group, Each group contains 10 mice, animal grouping and treatment methods are shown in Table 2

[0080] Table 2 Grouping and processing methods of animal experiments

[0081]

[0082]

[0083] The mice in Example 2 were weighed on the 13th day, then euthanized, the spleen was taken out and weighed wet to calculate the organ coefficient, and the organ coefficient of the mouse spleen was calculated according to the following formula:

[0084] Spleen organ coefficient = wet weight of spleen / weight of mouse before euthanasia

[0085] Experimental results such as figure 2 As shown, the results show that ...

Embodiment 3

[0086] Embodiment 3: Lactobacillus casei CCFM1052 can significantly reduce the levels of alanine aminotransferase (ALT) and γ-glutamyl transpeptidase (γ-GT) in the serum of PFOA exposed mice;

[0087] The mice in Example 3 were weighed on the 13th day, then euthanized, and the orbital blood was taken after 3000rpm / min centrifugation for 15min to obtain serum, and alanine aminotransferase (ALT) and γ-glutamyl in the serum were detected by an automatic biochemical analyzer Transpeptidase (γ-GT) content. ALT mainly exists in the soluble part of the hepatocyte protoplasm, and the increase of ALT activity indicates that the hepatocytes are destroyed and the permeability of the cell membrane is enhanced. Experimental results show that( image 3 ), taking Lactobacillus casei CCFM1052 can significantly reduce the levels of alanine aminotransferase (ALT) and γ-glutamyl transpeptidase (γ-GT) in the serum of PFOA-exposed mice. It shows that taking Lactobacillus casei 8-9 can significan...

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Abstract

The invention discloses multifunctional lactobacillus casei CCFM1052 for relieving the PFOA toxic effect, and fermented food and application of the multifunctional lactobacillus casei CCFM1052. The strain has the high adsorbing effect on PFOA and is not subjected to field planting in the intestinal tract, liver oxidative stress injury caused by the PFOA is significantly relieved, the serum biochemical indices are significantly decreased, spleen atrophy caused by PFOA exposure is significantly relieved, imbalance of microorganisms in the intestinal tract due to PFOA exposure is relieved, intestinal flora metabolic disturbance caused by PFOA exposure is relieved, the content of acetic acid and propionic acid in the intestinal tract is significantly increased, the fecal moisture content of constipation mice is increased, the time of first melena of the constipation mice is shortened, proliferation of INS-1 cells and expression of MafA genes under the high glucose effect are improved, thepotential for relieving PFOA-related diabetes is achieved, occurrence of liver diseases, metabolic diseases and potential carcinogenicity is reduced, and wide application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to multifunctional Lactobacillus casei CCFM1052 for alleviating the toxic effect of PFOA, fermented food and applications thereof. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal stability, chemical stability and biological stability. Used in ready-to-wear (such as waterproof, stain-resistant outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), takeaway food containers, personal care products (such as dental floss), and fire fighting foam. Among them, PFOA, as one of the final transformation products of various fluorine compounds, can be enriched along the food chain. PFOA has been detected in various environmental media around the world, such as water, soil, atmosphere, dust, etc., as well as in animals and humans, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L19/20A23C13/16A23C19/00A23C9/123A61K35/747A61P39/06A61P1/10A61P1/16A61P9/12A61P3/04C12R1/245
CPCA23L19/20A23C13/165A23C19/00A23C9/1234A61K35/747A61P39/06A61P1/10A61P1/16A61P9/12A61P3/04A23V2002/00C12R2001/245C12N1/205A23V2400/143A23V2200/30A23V2200/326A23V2200/332
Inventor 陈卫王刚梁席赵建新张灏
Owner JIANGNAN UNIV
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