Cervical cancer staging method based on cervical exfoliated cell gene detection, and detection kit

Abstract

The invention discloses a cervical cancer staging method based on cervical exfoliated cell gene detection. The cervical cancer staging method based on cervical exfoliated cell gene detection comprisesthe steps of acquiring a cervical exfoliated cell and a viable tissue, extracting whole genome deoxyribonucleic acid (DNA), detecting a gene, and carrying out immunohistochemical examination. The invention further discloses a cervical cancer staging detection kit based on cervical exfoliated cell gene detection. The kit comprises PCR reaction liquid containing a specific primer. According to thecervical cancer staging method based on cervical exfoliated cell gene detection and the cervical cancer staging detection kit based on cervical exfoliated cell gene detection provided by the invention, exfoliated cells of normal cervical epithelium, pre-cancerous diseased cervical epithelium and cervical cancer are subjected to DNA quantitative detection, so that the relevance of P53 and P16 and the cervical cancer occurrence and development is illuminated from the angle of the exfoliated cells, and a new basis is provided for the cervical cancer staging diagnosis; DNA in the cervical exfoliated cell is extracted so as to be detected, so that the advantage of low sampling quantity is realized; and materials are drawn simply, conveniently and non-invasively, a patient has no discomfort, anda sample to be detected can be obtained during the routine examination of the reproductive system diseases in the obstetrics and gynecology department, so that the cervical cancer staging method based on cervical exfoliated cell gene detection and the cervical cancer staging detection kit based on cervical exfoliated cell gene detection provided by the invention have the advantages of non-invasive material drawing, small side effect and good patient compliance.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a cervical cancer staging method and a detection kit based on the gene detection of cervical exfoliated cells. Background technique [0002] Cervical cancer is one of the most common malignant tumors in women. There are about 500,000 new cases and 250,000 deaths in the world every year. In my country alone, there are about 135,000 new cases every year on average, accounting for 1 / 4 of the total number of cases in the world. , and the number of deaths has reached an average of more than 50,000 per year; therefore, the prevention and treatment of cervical cancer is urgent. According to histopathological classification, cervical cancer is mainly divided into squamous cell carcinoma (squamous cell carcinoma for short) and adenocarcinoma, of which squamous cell carcinoma accounts for about 75-80%, and adenocarcinoma accounts for about 20-25%. Early detection of cervical can...

AI Technical Summary

Problems solved by technology

[0003] At present, cervical cancer screening generally uses a three-step diagnosis of cervical lesions (liquid-based cytology, colposcopy, and histopathological diagnosis) combined with HPV detection, but early diagnosis of cervical cancer is still difficult.

There are four main reasons for this: ① Liquid-based cytology examination does not clearly distinguish the grade of cervical cytopathy, and it is greatly affected by subjective factors. For example, the overlap of blood and mucus cells will affect the identification of abnormal cells, reducing the ② colposcopy and histopathological diagnosis are both invasive examination methods, and at the same time, there are problems that it is difficult to obtain materials in place, and it is difficult to obtain typical and sufficient pathological specimens; ③The sensitivity of HPV DNA detection is high, but the specificity is low. The positive result can only indicate whether there is a transient infection, and the value of predicting the occurrence and progression of cervical cancer needs to be further confirmed

[0004] In view of the limitations of the existing methods for screening cervical cancer, researchers have been trying to find new methods for early diagnosis from the protein and gene levels; in addition to immunohistochemistry, polymerase chain reaction (PCR) ), in situ hybridization and other conventional techniques, using new technologies such as DNA ploidy analysis, gene sequencing, and circulating free DNA detection to screen cervical cancer markers and use them for detection have been reported from time to time, but most of them are still in the scientific research stage. It cannot be used for clinical early screening, let alone the staging of cervical cancer; therefore, the development of non-invasive detection kits for early and staging diagnosis of cervical cancer is still a top priority

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