A bacterial strain capable of degrading organic amines and its application
An organic amine and substance technology, which is applied in the field of strains with the function of degrading organic amine substances, can solve the problems of high treatment cost and secondary pollution, and achieve the effects of large treatment capacity, reduced equipment use pressure and mild conditions.
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Embodiment 1
[0043] A new strain of Pseudomonas stutzeri (Pseudomonas stutzeri), its biological deposit number is CGMCCNo.17358, named YJY19-02, and its specific acquisition method is as follows:
[0044] Take 10mL of activated sludge from the Qingyuan environmental protection petrochemical pretreatment system in Boxing County, Binzhou, Shandong, and transfer it to the inorganic salt medium containing 50ppm triethylamine (inorganic salt medium (g / L): 0.09g KH 2 PO 4 , 0.22g K 2 HPO 4 , 0.26gNaH 2 PO 4 , 0.23g MgSO 4 , 0.28g CaCl 2 , 0.003g FeCl 3 , pH 7.0~7.2);
[0045] The domestication concentration of triethylamine, methylamine, ethylamine and diethylamine starts from 50ppm, and after the degradation is completed, continue to transfer 5% to the inorganic salt medium containing 100ppm triethylamine, methylamine, ethylamine and diethylamine In this method, gradually increase the concentration to 200ppm and 300ppm, and shake and culture each gradient at 37°C and 170rpm for 7 days; ...
Embodiment 2
[0050] A bacterial agent with the function of degrading organic amine substances. The bacterial agent is bacterial powder prepared from a strain with a biological deposit number of CGMCC No. 17358. 7 -5×10 10 indivual;
[0051] Its preparation method, concrete steps are as follows:
[0052] 1) Activation of strains; 2) Preparation of liquid seeds; 3) Fermentation; 4) Preparation of bacterial powder; wherein:
[0053] The 1) strain activation process is as follows: take 1-5 μL of frozen Pseudomonas stutzeri and inoculate it into a test tube containing 5 ml of LB liquid medium, and culture it at 30-35° C., 150-180 rpm for 16h-24h;
[0054] The 2) liquid seed preparation process is: transfer the activated test tube bacteria into 200ml LB liquid medium, and cultivate it at 30-35°C, 150-180rpm for 16h-24h;
[0055] The specific process of 3) fermentation is: inoculate the prepared liquid seeds into the fermenter according to the inoculation amount of 5-15vt% to carry out expande...
Embodiment 3
[0062] The strain obtained in Example 2 is subjected to a qualitative detection of the function of degrading organic amines, and the specific steps are as follows:
[0063] The formula of inorganic salt medium is: 0.09g KH 2 PO 4 , 0.22g K 2 HPO 4 , 0.26g NaH 2 PO 4 , 0.23g MgSO 4 , 0.28g CaCl 2 , 0.003g FeCl 3 , pH 7.0-7.2. The seed solution in Example 2 was inoculated in the inorganic salt liquid medium whose initial concentration of organic amine was 300ppm with the inoculation amount of 1‰, 5‰, and 1%, and the fresh seed solution was inoculated in the organic In LB medium with an initial concentration of amine of 300ppm, culture was continuously shaken at 37°C and 170r / min for 3 days, and samples were taken every 24 hours to detect changes in the content of organic amines. The following table is the relevant detection data when the organic amines are respectively triethylamine, methylamine, ethylamine and diethylamine:
[0064] Table 1 Triethylamine degradation s...
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