Bacillus amyloliquefaciens and application thereof in agricultural production

A technology of amylolytic spores and bacillus, applied in the direction of application, microbe-based methods, chemicals for biological control, etc., can solve the problem that the effect of disease prevention and treatment cannot be stable or lasting, and achieve obvious effects of promoting wheat growth and increasing yield , The effect of improving production performance

Active Publication Date: 2020-01-31
山东蔚蓝生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, many bacteria, actinomycetes and fungi prevent the occurrence and spread of diseases by producing antibiotics or competing for nutri

Method used

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  • Bacillus amyloliquefaciens and application thereof in agricultural production
  • Bacillus amyloliquefaciens and application thereof in agricultural production
  • Bacillus amyloliquefaciens and application thereof in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 strain screening and identification

[0032] 1. Strain screening:

[0033] (1) Soil samples: collected from the soil in the Minsk region of Belarus.

[0034] (2) Prepare soil dilution by gradient dilution method, then carry out plate coating and streak culture, and isolate and obtain multiple strains of soil microorganisms; then further use the transparent circle method to screen out the opposite Rhizoctonia solani, Fusarium oxysporum and Heliconia The three strains with the most significant antibacterial effect on pathogenic bacteria such as Sporocoelus were named VB1263, VB1264, and VB1266 respectively.

[0035] 2. Strain identification:

[0036] Morphological characteristics of VB1266 strain: the colony is round, beige, 3-5mm in diameter, the edge of the colony is smooth, moist, shiny, and raised in the middle; the bacteria are short straight rods, Gram-positive bacteria; Oval, partly round; sporangia do not expand; cells exist alone, in pairs or in shor...

Embodiment 2

[0040]Example 2 Bacillus amyloliquefaciens VB1266 antibacterial ability evaluation

[0041] 1. Preparation of bacteria solution

[0042] First activate Bacillus amyloliquefaciens VB1266, then pick the activated Bacillus amyloliquefaciens VB1266 and inoculate it in LB liquid medium, cultivate it at 37°C and 220r / min for 14 hours, and obtain a viable count of 10 8 -10 9 CFU / ml of bacterial liquid.

[0043] 2. Preparation of pathogenic bacteria

[0044] A total of 10 species of Columella graminearum, Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, Fusarium graminearum, Rhizoctonia graminearum, Strawberry anthracnose, Botrytis cinerea, Alternaria and Pythium Pathogens (provided by the Institute of Plant Protection, Shandong Academy of Agricultural Sciences) were inoculated on PDA medium, cultured at 30°C for 5 days, and then used for later use.

[0045] 3. Plate antibacterial test

[0046] Inoculate a pathogenic bacteria cake with a diameter of 7 mm in the cente...

Embodiment 3

[0051] Example 3 Application of Bacillus amyloliquefaciens VB1266 in the prevention and treatment of corn sheath blight

[0052] 1. Preparation of Bacillus amyloliquefaciens VB1266 bacterial powder

[0053] Perform liquid fermentation of Bacillus amyloliquefaciens VB1266 in a 5-ton fermenter, and stop the fermentation when the microscopic examination spore rate reaches 90% or more; centrifuge at 5000 rpm for 10 minutes, remove the fermentation supernatant, and spray-dry the sludge to obtain live bacteria The amount of bacteria powder is 1 billion / g.

[0054] 2. Preparation of Rhizoctonia solani spore suspension

[0055] Inoculate Rhizoctonia solani into PDA medium and culture at 30°C for 5 days until the entire plate is covered. Use a sterilized hole puncher (inner diameter d=5mm) to punch holes on the cultured bacteria plate to make bacteria cakes, and then use sterile inoculation needles to inoculate 5 pieces of pathogenic bacteria cakes into 100ml PDA for culture culture...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, particularly provides a new strain of bacillus amyloliquefaciens VB1266with the preservation numberbeing CCCTCC No: M2019440, and provides application of the bacillus amyloliquefaciens VB1266 in agricultural production. The bacillus amyloliquefaciensis screened from the soil of Minsk, Belarus and has a high inhibiting effect on common pathogens such as cochliobolus sativus, fusarium oxysporum, rhizoctonia solani, fusarium moniliforme and fusarium graminearums, common crop diseases can be effectively controlled and treated, the crop yield is improved, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a bacillus amyloliquefaciens and its application in agricultural production. Background technique [0002] The relationship between microorganisms and agriculture is very close. In the soil known as the "microbial base camp", microorganisms play a major role in the quality cycle and have an irreplaceable role. They break down plant and animal waste and convert it into humus, which contributes to the formation of good soil structure. Many soil microorganisms can fix nitrogen in the air and transform various organic matter, and continuously provide plants with various nutrients such as carbon, nitrogen, phosphorus, and potassium that can be effectively used. Pathogenic microorganisms of insects and antagonistic microorganisms of plant pathogens also widely exist in nature, and they can be used in the control of plant diseases and insect pests to partiall...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N63/27A01N63/28A01N63/20A01N63/34A01N63/30A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 苑伟伟凌红丽周英俊吕宾梁莉孙文丽魏焕巍
Owner 山东蔚蓝生物科技有限公司
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