Electrochemical sensor for detecting aflatoxin

An aflatoxin and electrochemical technology, applied in the field of biosensors, can solve the problems of poor sensitivity and repeatability of thin-layer chromatography, cumbersome pretreatment steps, and inapplicability for rapid detection, and achieve easy portable and online detection, samples and reagents. The effect of low consumption and large distribution of electroactive sites
CN110794013AInactive Publication Date: 2020-02-14INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
Publication Date
2020-02-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an electrochemical sensor for detecting aflatoxin. The sensor is based on the principle that aflatoxin can be subjected to non-covalent bond combination with an acetylcholin esterase active site to inhibit the activity of the enzyme. The surface of a screen-printed electrode is modified with a prussian blue-graphene compound and a nanogold-chitosan compound; and acetylcholin esterase is dropwise added to the modified electrode, so that the electrochemical sensor capable of detecting aflatoxin is obtained. The sensor has advantages of simple operation, low cost, and highdetection sensitivity. Compared with the traditional detection method, the electrochemical sensor has the following characteristics: the reaction time is short; the sample and reagent consumption islow; the stability is high; portability is high; the sensor can be applied to field detection of actual samples; and the requirements for rapid aflatoxin detection technology development and internationalization in China are met.
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Description

technical field

[0001] The invention relates to an electrochemical sensor for detecting aflatoxin, belonging to the field of biosensors. Background technique

[0002] Aflatoxin (AFs) is a highly toxic mycotoxin with high teratogenicity and mutagenicity. It is ubiquitous in grains, peanuts, corn, cottonseed meal, soybean meal, etc., and it is in the field and storage process can generate aflatoxin. Aflatoxins are classified as Class I carcinogens by the International Agency for Research on Cancer, which are extremely harmful to humans, poultry and livestock. Among them, aflatoxin B1 (AFB1) is the most toxic, and its toxicity is 67 times that of arsenic.

[0003] The current detection techniques for aflatoxin include enzyme-linked immunoassay, gas chromatography-mass spectrometry, liquid chromatography, thin-layer chromatography, liquid chromatography-mass spectrometry, etc. Among them, enzyme-linked immunoassay has good specificity, but needs to prepare specific antibodies,...

Claims

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