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Microchromodispora fungus hnu107 and its application in the degradation of ammonia waste gas

A technology of HNU107 and Chromodispora, which is applied in the field of environmental pollution control, can solve the problems of difficult preservation, rapid degradation, and low efficiency, and achieve the effects of shortening domestication time, good storability, and improved removal efficiency

Active Publication Date: 2021-07-27
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current bio-trickling filter reactors have disadvantages such as low efficiency, rapid degradation, and difficult storage, which to a certain extent limit the application of bio-trickling filter reactors in the field of exhaust gas purification.

Method used

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  • Microchromodispora fungus hnu107 and its application in the degradation of ammonia waste gas
  • Microchromodispora fungus hnu107 and its application in the degradation of ammonia waste gas
  • Microchromodispora fungus hnu107 and its application in the degradation of ammonia waste gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation and cultivation of the microchromodispora fungus "HNU107"

[0035] The microchromodispora fungus "HNU107" is isolated from the bioreactor of Rousselot (Wenzhou) Gelatin Co., Ltd. The design structure of the biotrickling filter reactor is as follows: figure 1 shown.

[0036] The separation method is as follows: Take 5 g of domesticated bioreactor sludge, dilute it with distilled water to three different concentration gradients of 10 times, 100 times and 1000 times, and spread it on the PDA fungal solid medium respectively. Each petri dish was coated with one milliliter of the diluted sludge solution, cultured at a constant temperature and dark at 25 degrees, and observed the independent colonies formed after culturing for one week. Pick the mycelium from the edge of the selected colony, transfer to a fresh PDA plate for streak separation and culture, repeat until a pure culture is obtained, and transfer to a PDA slant for storage.

Embodiment 2

[0037] Example 2: Morphological and molecular biological identification of the fungus of the genus Leuchromodispora "HNU107"

[0038] Under a microscope, observe the characteristics of endophytic fungi such as colonies, hyphae, and spores, and identify them by referring to the "Handbook of Fungal Identification". Specifically, the screened strains were inoculated on a PDA plate by spot seeding method, cultured at a constant temperature of 28 degrees, and the morphological characteristics of the strains were observed with the naked eye, including colony shape, color, size, edge characteristics, mycelial properties, and growth speed. and other features. The hyphae on the surface of the colony were picked and placed on an optical microscope (Japan, OLYMPUS) to observe the presence or absence of spores and sporulation structures.

[0039] The morphological characteristics of the microchromodispora fungus "HNU107" of the present invention are as follows:

[0040] like figure 2 an...

Embodiment 3

[0041] Example 3: Molecular Biological Identification of Microchromodispora Fungus "HNU107"

[0042] Genomic DNA of Leuchromodispora fungus "HNU107" was extracted by general fungal DNA extraction method. The extraction method is as follows: take 1.5mL bacterial liquid, 12000×g, 2min, collect the bacterial cells; add 500μL 5×CTAB (containing 1% β-mercaptoethanol), quick-freeze in liquid nitrogen for 30s, then transfer to 65°C for 30s, Repeat the above process 3 times; vortex at high speed for 3-5min, incubate at 65°C for 20min, add 2mL of phenol:chloroform:isoamyl alcohol (25:24:1, V / V / V), 12000×g, 10min, take Supernatant: Add 2 times the volume of absolute ethanol to the supernatant, centrifuge again after standing still for 20 minutes; pour off the supernatant, wash the precipitate with 70% alcohol; dry under natural conditions to obtain fungal DNA for later use.

[0043] Using fungal universal primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3')...

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Abstract

The invention discloses the microchromodispora fungus HNU107 and its application in ammonia waste gas degradation. The Microdiplodia sp. fungal strain "HNU107" of the present invention has a preservation number of CCTCC NO: M 2019501. Application of the fungus "HNU107" in the degradation of ammonia-containing waste gas. Artificially increasing the abundance of HNU107 in the bioactive filler can improve the removal efficiency of the bioactive filler for ammonia-containing waste gas. The reactor added with the fungus HNU107 of the genus Leuchromodispora, after entering the stable period, the absorption and removal efficiency of ammonia-containing waste gas increased by 12.8% compared with the control group. The acclimatization time of the active filler after adding the fungus HNU107 of the genus Leuchromodispora was shortened by 3 days, and the biotrickling filter reactor could be quickly started.

Description

technical field [0001] The technology belongs to the field of environmental pollution control, and relates to a microdiplodia sp. nitrifying fungus "HNU107" and its application in the biodegradation of ammonia-containing industrial waste gas. Background technique [0002] Air pollution is one of the most serious environmental problems at present. With the development of the economy, industrial emissions are the main cause of air pollution. According to existing research reports, in the actual industrial waste gas emissions, ammonia-containing waste gas emissions account for an important proportion, so it is listed as one of the national priority control gas pollutants. Ammonia gas has a strong pungent smell, which has a great impact on surrounding residents, and the discharge of a large amount of ammonia-containing waste gas seriously damages human health. In addition, ammonia discharged into the atmosphere forms aerosols with water, which is the main cause of acid rain. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B01D53/84B01D53/58C12R1/645
CPCB01D53/58B01D53/84B01D2251/95C12N1/14C12N1/145C12R2001/645Y02A50/20
Inventor 刘奇沈晨佳倪建国成卓韦宋欣欣杨华云李伟东
Owner HANGZHOU NORMAL UNIVERSITY
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