Extraction method of morchella esculenta polysaccharide and application thereof
An extraction method, a morel technology, is applied in the field of morel polysaccharide extraction, which can solve the problems of easy reduction of polysaccharide biological activity, low extraction efficiency, cumbersome procedures, etc., and achieve strong biological activity, high content, and simple extraction process Effect
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Embodiment 1
[0022] Step 1, fermentation: add 5 to 7 times of water after the dried Morchella sporocarp is crushed to expand, and add 1% (w / w) of Bacillus subtilis (hereinafter referred to as spores) that can secrete chitinase by saccharomyces bacillus) for shaker fermentation. The weight ratio of yeast to bacillus is 1:2, the fermentation temperature is 29°C, the pH value is 4.5, and the fermentation time is 16 hours.
[0023] Step 2, enzymatic hydrolysis: After fermenting for a period of time, mix the fermentation broth evenly, and add a compound enzyme preparation, which is made by mixing chitinase and acid protease at a weight ratio of 1:1. The addition amount of the compound enzyme preparation is 1.5%, the action temperature is 36°C, the pH value is 4.2, and the action time is 12 hours.
[0024] Step 3: Centrifuge the enzymolysis solution obtained in step 2, take the supernatant and concentrate it, then add 4 times the volume of absolute ethanol to obtain the alcohol analyte, and the...
Embodiment 2
[0027] Step 1, fermentation: add 5-7 times of water expansion after the dried hickory chick sporocarp is pulverized, and add 3% (w / w) yeast and Bacillus subtilis (hereinafter referred to as Bacillus for short) that can secrete chitinase ) of the compound bacterial agent for shaker fermentation. The weight ratio of yeast to bacillus is 1:2, the fermentation temperature is 31° C., the pH value is 4.0, and the fermentation time is 14 hours.
[0028] Step 2, enzymatic hydrolysis: After fermenting for a period of time, mix the fermentation broth evenly, and add a compound enzyme preparation, which is made by mixing chitinase and acid protease at a weight ratio of 1:1. The addition amount of the compound enzyme preparation is 1.75%, the action temperature is 38°C, the pH value is 3.9, and the action time is 10 hours.
[0029] Centrifuge the enzymolysis solution obtained in step 2, take the supernatant and concentrate it, then add 4 times the volume of absolute ethanol to obtain the...
Embodiment 3
[0032] Step 1, fermentation: crush the fruiting body of the dried hickory chick and add 5-7 times of water to expand, and add 5% (w / w) yeast and Bacillus subtilis capable of secreting chitinase (hereinafter referred to as Bacillus) The compound bacterial agent was fermented on a shaking table. The weight ratio of yeast to bacillus is 1:2, the fermentation temperature is 33°C, the pH value is 3.5, and the fermentation time is 12 hours.
[0033] Step 2, enzymatic hydrolysis: After fermenting for a period of time, mix the fermentation broth evenly, and add a compound enzyme preparation, which is made by mixing chitinase and acid protease at a weight ratio of 1:1. The addition amount of the compound enzyme preparation is 2.0%, the action temperature is 40°C, the pH value is 3.5, and the action time is 8 hours.
[0034] Step 3: Centrifuge the enzymolysis solution obtained in step 2, take the supernatant and concentrate it, then add 4 times the volume of absolute ethanol to obtain ...
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