Skin beautifying and senility resisting preparation and preparation method thereof

A kind of preparation and autologous technology, applied in the field of biomedicine, can solve the problems that the direction and state of proliferation cannot be well controlled, cannot quickly adapt to the internal environment, and the activity of fibroblasts is poor, so as to achieve uniform and rapid repair of wrinkles, Enhanced wrinkle repair effect, orderly and uniform effect of proliferation

Inactive Publication Date: 2020-02-28
GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the injections in the prior art have the following problems: the fibroblasts suspended in the electrolyte have poor activity and cannot quickly adapt to the internal environment after being injected subcutaneously, resulting in a large number of cell death and inflammatory reactions; fibroblasts injected into the subcutaneous Finally, the direction and state of proliferation cannot be well controlled, resulting in uneven filling of fine lines; the injection requires electrolytes to maintain osmotic pressure, and injecting a large amount of electrolytes into the skin will burden normal tissues, easily causing infection and edema etc.

Method used

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  • Skin beautifying and senility resisting preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of autologous fibroblasts

[0037] Pretreatment: Put the collected skin tissue into a petri dish filled with alcohol and soak it for 1 min, wash it with normal saline with 1% double-antibody for 3-5 times, and remove the fat tissue with hemostatic forceps and scissors. Remove cleanly. Double antibody refers to a solution containing penicillin and streptomycin, in which penicillin is 100IU / ml and streptomycin is 100IU / ml.

[0038] Separate the dermis and epidermis: cut the skin tissue into 2×3mm 2 Strips of the same size were digested with 0.25% β-enzyme at 37°C for 1 hour. After digestion is complete, the epidermis is removed and rinsed with normal saline.

[0039] Primary culture: After the dermis is infiltrated with medium containing cytokines, the bottom layer of the dermis (the side where the dermis is in contact with the fat) is spread on the bottom of the T25 culture bottle, and an appropriate interval is left between the dermis to facilitate tiss...

Embodiment 2

[0047] (1) Preparation of autologous fibroblasts

[0048] Pretreatment: Put the collected skin tissue into a petri dish filled with alcohol and soak it for 1 min, wash it with normal saline with 1% double-antibody for 3-5 times, and remove the fat tissue with hemostatic forceps and scissors. Remove cleanly. Double antibody refers to a solution containing penicillin and streptomycin, in which penicillin is 100IU / ml and streptomycin is 100IU / ml.

[0049] Separate the dermis and epidermis: Digest with 4 mg / ml dispase II (Dispase II, neutral protease) at 37°C for 2 hours. After the digestion is complete, remove the epidermis and rinse with normal saline.

[0050] Primary culture: After the dermis is infiltrated with medium containing cytokines, the bottom layer of the dermis (the side where the dermis is in contact with the fat) is spread on the bottom of the T25 culture bottle, and an appropriate interval is left between the dermis to facilitate tissue cells to crawl out. Put t...

Embodiment 3

[0058] This example is basically the same as Example 1, the difference lies in the composition content of the preparation: take 6ml of PRP and 1ml hyaluronic acid for injection and blow the autologous fibroblasts evenly, the content of autologous fibroblasts is 3×10 7 pcs / ml, drawn with a sterile syringe and sealed for use.

[0059] In addition, in the primary culture step, the cytokines and concentrations in the culture medium are respectively: 120ng / ml FGF, 15ng / ml TGF-β and 60ng / ml IGF-I.

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Abstract

The invention belongs to the field of biological medicines, and particularly relates to a skin beautify and senility resisting preparation and a preparation method thereof. The skin beautifying and senility resisting preparation comprises autologous platelet-rich plasma, hyaluronic acid and autologous fibroblasts. The preparation has that advantages that the preparation has a long-acting wrinkle repairing function and is high in safety and good in repairing effect. The autologous platelet-rich plasma is separated from venous blood in a patient, and the autologous fibroblasts are separated fromdermis of the patient and have the advantage of low immunogenicity. The preparation has good effects on skin repairing, wrinkle removing, skin beautify and senility resisting and wide application prospects and can be applied to practical operations of skin injury repair.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a cosmetic and anti-aging preparation and a preparation method thereof. Background technique [0002] The skin contains multiple structures such as the epidermis, dermis, and appendages. With age and the influence of external factors, the water content of the skin decreases, the loss of collagen, and the appearance of tissue segments at the base of the skin will lead to wrinkles. The appearance of wrinkles can affect your appearance and mood, and there are many products on the market aimed at alleviating wrinkles. [0003] Chinese patent CN108066820A (an injection of autologous dermal fibroblasts) discloses an injection of autologous dermal fibroblasts and a preparation method, which can be used to treat wrinkles of weak dermis. The injection includes autologous dermal fibroblast living cells and an injection matrix, and the injection matrix includes electrolytes such as s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/99A61K8/73A61Q19/08
CPCA61K8/735A61K8/983A61K8/985A61K8/99A61K2800/592A61K2800/91A61Q19/08
Inventor 边曙光张凤宁汪国云曾智芃明凯
Owner GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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