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Preparation method of L-lysine sulfate and by-products thereof

A technology of lysine sulfate and by-products, applied in the field of lysine production technology, can solve the problems of large environmental pollution and high production cost, and achieve the effects of short process route, low cost and high content

Pending Publication Date: 2020-03-10
黑龙江成福食品集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a kind of preparation method of L-lysine sulfate and by-product thereof, solves the problems that the existing direct fermentation method production process has bigger environmental pollution and high production cost

Method used

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  • Preparation method of L-lysine sulfate and by-products thereof

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Embodiment 1

[0020] The present embodiment provides the preparation method of L-lysine sulfate and by-product thereof, comprises the following steps:

[0021] (1) medium sterilization: get fermentation medium, the component of described fermentation medium is: glucose 150g / L, molasses 100g / L, corn steep liquor 15g / L, ammonium sulfate 50g / L, phosphoric acid 1g / L, Potassium chloride 1.5g / L, magnesium sulfate 0.5g / L, ferrous sulfate 0.01g / L, manganese sulfate 0.1g / L, VB1200μg / L, calcium carbonate 40g / L; Before use, ferment medium with 20% NaOH Adjust the pH to 7.0, and then use steam at 125°C to continuously heat the medium to cause the death of microorganisms;

[0022] (2) Fermentation: Insert the Corynebacterium glutamicum into the fermentation medium under aseptic conditions, the insertion amount is 17% of the weight of the medium, the fermentation process control temperature is 35-40 ° C, the pH value is 6.9 and the DO value 25%, the fermentation time is 54 hours, so that Corynebacterium...

Embodiment 2

[0025] The present embodiment provides the preparation method of L-lysine sulfate and by-product thereof, comprises the following steps:

[0026] (1) medium sterilization: get fermentation medium, the component of described fermentation medium is: glucose 150g / L, molasses 100g / L, corn steep liquor 15g / L, ammonium sulfate 50g / L, phosphoric acid 1g / L, Potassium chloride 1.5g / L, magnesium sulfate 0.5g / L, ferrous sulfate 0.01g / L, manganese sulfate 0.1g / L, VB1200μg / L, calcium carbonate 40g / L; Before use, ferment medium with 20% NaOH Adjust the pH to 7.0, and then use 130°C steam to continuously heat the medium to cause the death of microorganisms;

[0027] (2) Fermentation: Insert the Corynebacterium glutamicum into the fermentation medium under aseptic conditions, the insertion amount is 15% of the weight of the medium, the fermentation process control temperature is 35-40 ° C, the pH value is 7.0 and the DO value 20%, the fermentation time is 60 hours, so that Corynebacterium gl...

Embodiment 3

[0030] The present embodiment provides the preparation method of L-lysine sulfate and by-product thereof, comprises the following steps:

[0031] (1) medium sterilization: get fermentation medium, the component of described fermentation medium is: glucose 150g / L, molasses 100g / L, corn steep liquor 15g / L, ammonium sulfate 50g / L, phosphoric acid 1g / L, Potassium chloride 1.5g / L, magnesium sulfate 0.5g / L, ferrous sulfate 0.01g / L, manganese sulfate 0.1g / L, VB1200μg / L, calcium carbonate 40g / L; Before use, ferment medium with 20% NaOH Adjust the pH to 7.1, and then use 120°C steam to continuously heat the medium to cause the death of microorganisms;

[0032] (2) Fermentation: Insert the Corynebacterium glutamicum into the fermentation medium under aseptic conditions, the insertion amount is 18% of the weight of the medium, the fermentation process control temperature is 35-40 ° C, the pH value is 6.8 and the DO value 30%, the fermentation time is 48 hours, so that Corynebacterium gl...

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Abstract

The invention discloses a preparation method of L-lysine sulfate and by-products thereof, and belongs to the technical field of lysine production processes. The preparation method comprises the following steps: adjusting the pH value of a fermentation medium to 7.0-7.1 by using 20% NaOH, then continuously heating the medium by using steam to cause microbial death, inoculating lysine producing bacteria in a sterile state, carrying out fermentation at a temperature of 35-40 DEG C, a pH value of 6.8-7.0 and a DO value of 20-30% for 48-60 hours, after the fermentation is finished, putting the mixture of lysine and the substrate into an isoelectric extraction workshop, adjusting the pH value of the fermentation liquid to 5-6 by using sulfuric acid, filtering the treated fermentation liquid by using ceramic membrane equipment, performing triple-effect concentration on the filtered clear liquid, and performing granulation and drying by using a guniting granulation bed to obtain a finished product. According to the preparation method, the environmental protection problem is solved, the production processes are reduced, the process route is simple, the consumption of raw materials and auxiliary materials is low, the cost is low, and the produced lysine content is high.

Description

technical field [0001] The invention relates to the technical field of lysine production technology, in particular to a preparation method of L-lysine sulfate and by-products thereof. Background technique [0002] The chemical name of lysine is 2,6-diaminocaproic acid. Lysine is a basic essential amino acid. Since the content of lysine in grain food is very low, and it is easily destroyed and lacked during processing, it is called the first limiting amino acid. [0003] Lysine is one of the essential amino acids for humans and mammals. The body cannot synthesize it by itself and must be supplemented from food. Lysine is mainly found in animal foods and beans, and the content of lysine in cereal foods is very low. Lysine has positive nutritional significance in promoting human growth and development, enhancing the body's immunity, anti-virus, promoting fat oxidation, and relieving anxiety. It can also promote the absorption of certain nutrients and can work synergistically...

Claims

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Application Information

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IPC IPC(8): C12P13/08C07C227/40C07C229/26C12R1/15
CPCC12P13/08C07C227/40C07B2200/07C07C229/26
Inventor 王成福唐瑞
Owner 黑龙江成福食品集团有限公司
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