L-threonine fermentation process optimization method
A fermentation process and optimization method technology, applied in the field of amino acid fermentation, can solve the problems of low added value of products, and achieve the effects of improving cell density, metabolic efficiency and mass transfer rate.
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Embodiment 1
[0031] L-threonine fermentation process optimization method, it comprises the following steps:
[0032] Step 1) Prepare fermentation medium: 60g / L sucrose, 30g / L glucose, 20g / L corn steep liquor, 5g / L ammonium sulfate, 0.5g / L potassium dihydrogen phosphate, 0.5g / L dipotassium hydrogen phosphate, heptahydrate Magnesium sulfate 0.1g / L, ferrous sulfate heptahydrate 10mg / L, manganese sulfate monohydrate 10mg / L, V B1 2mg / L, V H 50μg / L.
[0033] Step 2) Fermentation: Put the seed liquid of the L-threonine-producing strain (Trfc as an example) into a fermenter containing a fermentation medium at an inoculation amount of 1.5%, and ferment at an inoculation density of OD 600 The temperature is 0.4, the temperature is 36°C, the stirring speed is 300-500rpm, the dissolved oxygen is controlled to 20% by aeration and stirring, and the foam is used to defoam, the fermentation time is 36h, the fermentation is stopped, and the fermentation liquid is collected;
[0034] Feed liquid needs ...
Embodiment 2
[0041] L-threonine fermentation process optimization method, it comprises the following steps:
[0042] Step 1) Prepare fermentation medium: 60g / L sucrose, 30g / L glucose, 20g / L corn steep liquor, 5g / L ammonium sulfate, 0.5g / L potassium dihydrogen phosphate, 0.5g / L dipotassium hydrogen phosphate, heptahydrate Magnesium sulfate 0.1g / L, ferrous sulfate heptahydrate 10mg / L, manganese sulfate monohydrate 10mg / L, V B1 2mg / L, V H 50μg / L.
[0043] Step 2) Fermentation: Put the seed liquid of the L-threonine-producing strain (Trfc as an example) into the fermenter containing the fermentation medium according to the inoculum amount of 2% for fermentation, and the inoculation density is OD 600 The temperature is 0.5, the temperature is 36°C, the stirring speed is 300-500rpm, the dissolved oxygen is controlled to 15% by ventilation and stirring, and the foam is used to defoam. The fermentation time is 36h, the fermentation is stopped, and the fermentation liquid is collected;
[0044...
Embodiment 3
[0058] Effects of various factors on threonine production and sugar-acid conversion in fermentation broth.
[0059] 1. On the basis of the comparative example, to verify the effect of hydrogen peroxide on fermentation, set the flow rate of hydrogen peroxide in each liter of fermentation broth as: 0, 1, 2, 3, 4, 5, unit ml / h, such as figure 1 As shown, the subsequent increase of the hydrogen peroxide flow rate increases the threonine production, and when it is lower than 2ml / h, the threonine production increases faster, and the sugar-acid conversion rate has little effect (not shown in the figure), and the flow rate is 2ml / h h, the production of threonine was close to the peak value, and the flow rate of hydrogen peroxide continued to increase, which had little effect on threonine. The possible reason is that hydrogen peroxide has a positive effect on the proliferation of bacteria, which leads to an increase in the production of threonine, but has no significant impact on the s...
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