Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial agent for hexavalent chromium pollution treatment and hexavalent pollution treatment method

A microbial inoculum and pollution control technology, which can be used in microorganism-based methods, biological water/sewage treatment, biochemical equipment and methods, etc., and can solve problems such as high cost, secondary pollution, and difficult operation.

Active Publication Date: 2020-03-31
SHANDONG UNIV OF SCI & TECH
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many traditional chromium pollution control methods, such as physical method, adsorption method, chemical reduction method, ion exchange, electrolysis method, etc., but these methods have disadvantages such as high cost, difficult operation, easy to produce secondary pollution, etc., and it is difficult to deal with large Flow low concentration of harmful pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial agent for hexavalent chromium pollution treatment and hexavalent pollution treatment method
  • Microbial agent for hexavalent chromium pollution treatment and hexavalent pollution treatment method
  • Microbial agent for hexavalent chromium pollution treatment and hexavalent pollution treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and Identification of Example 1 Bacterial Strain 4-1

[0024] The specific screening scheme is as follows:

[0025] Take 1g of oilfield contaminated soil (collected in Shengli Oilfield, Dongying City, China in September 2018) and add it to the screening medium, shake it in a shaker with a temperature of 30°C and a rotation speed of 160rpm for one week, and then inoculate with 5% The culture medium was transferred to fresh medium to continue the culture, and the transfer was continued for 3 times under the same conditions. Then take the culture solution for gradient dilution, pick colonies with different shapes, and use the screening medium for degradation experiments.

[0026] in:

[0027] Screening medium: add 30mg·L on the basis of LB medium -1 Cr(VI).

[0028] LB medium: peptone 10g, yeast powder 5g, NaCl 5g, distilled water 1000mL, pH 7.2-7.5.

[0029] After isolation and screening, a strain 4-1 with stable resistance to chromium was obtained, and the...

Embodiment 2

[0030] The identification of embodiment 2 bacterial strains

[0031] 1. Morphological characteristics: the colony is moist, smooth and light yellow in color.

[0032] 2. Biological and biochemical characteristics

[0033] (1) Gram staining

[0034] A: Dyeing agent:

[0035] 1. Crystal violet mixed solution (A solution: crystal violet 2g, 95% ethanol 20ml; B solution: ammonium oxalate 0.8g, distilled water 80ml. Mix A and B, stand still for 48 hours and filter before use);

[0036] 2. Liquid iodine (1g of iodine, 2.0g of potassium iodide, 300mL of distilled water, first dissolve potassium iodide with 3-5mL of distilled water, then put in iodine tablets, after dissolving, add water to dilute to 300mL);

[0037] 3. Decolorization solution (95% ethanol 70mL);

[0038] 4. Counterstain solution (0.5% safranin aqueous solution).

[0039] B: Dyeing steps:

[0040] 1. Pick up a little bacterial lawn with an inoculation needle, spread it on a clean glass slide with a drop of steri...

Embodiment 3

[0076] Example 3: The enrichment ability of Stenotrophomonas acidaminiphila 4-1 to hexavalent chromium in wastewater containing hexavalent chromium

[0077] with K 2 Cr 2 o 7 Prepare Cr(VI) concentration to be 15mg L -1 , 50mg·L -1 , 100mg·L -1 , 120mg·L -1 , 150mg·L -1 , 180mg·L -1 , 200mg·L -1 , 500mg·L -1 , 1000mg·L -1 , the inoculation amount of bacterial strain 4-1 was 10% (v / v, and the bacterial density was 4.1×10 7 CFU / mL), the shaker rotates at 160 rpm and cultured at 30°C for 7 days, and the absorbance is measured at 540 nm with a visible spectrophotometer.

[0078] The specific measurement method is as follows: the culture medium is centrifuged at 4000r / min for 20min, 100μL of the supernatant is taken with a pipette gun and diluted with water to the mark in a 50mL colorimetric tube, 0.5mL of 1+1 sulfuric acid solution is added, 0.5mL of 1+ 1 phosphoric acid solution, and 2 mL of chromogen (weigh 0.2 g of diphenylcarbazide and dissolve it in 50 mL of aceto...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microbial remediation of heavy metal chromium in environment, particularly to a microbial agent for hexavalent chromium pollution treatment. The effective component of the microbial agent is (stenotrophomonas acidaziphi) 4-1 or fermentation liquor of (stenotrophomonas acidaziphi) 4-1.

Description

technical field [0001] The invention relates to the technical field of microbial remediation of heavy metal chromium in the environment, in particular to a microbial agent for hexavalent chromium pollution control and a hexavalent chromium pollution control method. Background technique [0002] Chromium is a toxic heavy metal widely used in metallurgy, mining, electroplating, printing and dyeing and other industries. The environmental pollution caused by chromium and its compounds mainly comes from inferior cosmetic raw materials, leather preparations, chrome-plated parts of metal parts, industrial pigments, tanned leather, rubber and ceramic raw materials. Chromium wastewater and chromium slag leachate are two major pollution sources of heavy metal chromium, which have a serious impact on soil, surface water, and shallow groundwater. Studies have shown that Cr(VI) is easily absorbed by the human body, causing damage to the skin and mucous membranes, respiratory, digestive,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C02F101/22C12R1/01
CPCB09C1/10C02F3/34C02F2101/22C12N1/20C12N1/205C12R2001/01
Inventor 李琳吕宪俊胡术刚申宪伟肖新峰薛建良高洪阁高宇尚秀芳
Owner SHANDONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com