Single cell array chip and screening method for ion channel drugs

Abstract

The invention discloses a single cell array chip and a screening method for ion channel drugs. The single cell array chip includes a lower layer micro-well chip and an upper layer micro-channel chip,and the lower layer micro-well chip and the upper layer micro-channel chip are bonded together; the upper surface of the lower layer micro-well chip is provided with micro-pits, and the center distances of the adjacent micro-pits are 30-90 [mu]m; and the material of the single cell array chip is selected one or more of high-molecular polymer, silicon, glass, quartz and paper. The method for screening ion channel drugs by utilizing the single cell array chip can obtain higher single cell capture rates, and the heterogeneity of single cells can be fully considered; and the screening method can enhance the accuracy and reproducibility of single cell level drug screening results, significantly reduce false positive rates / false negative rates and increase the screening efficiency of the ion channel drugs.

Description

technical field [0001] The invention relates to a single cell array chip, and also relates to a screening method for ion channel drugs. Background technique [0002] Ion channels are a class of transmembrane biomacromolecules with selective permeability to ions, which regulate various physiological activities of the body. Ion channel dysfunction can induce a variety of "ion channelopathies", such as epilepsy, arrhythmia, diabetes and so on. Drug development targeting ion channels is a hot topic in academia and industry. [0003] Traditional ion channel drug screening platforms include traditional calcium fluorescence high-throughput screening technology (FlexStation 3) and whole-cell patch clamp technology. Problems such as expensive equipment. The traditional calcium fluorescence high-throughput screening technology measures the total fluorescence value of all cells in a single well of a well plate (such as a 96-well plate). First, the number of cells is uncertain. In ad...

AI Technical Summary

Problems solved by technology

[0003] Traditional ion channel drug screening platforms include traditional calcium fluorescence high-throughput screening technology (FlexStation 3) and whole-cell patch clamp technology. Problems such as expensive equipment

The traditional calcium fluorescence high-throughput screening technology measures the total fluorescence value of all cells in a single well of a well plate (such as a 96-well plate). First, the number of cells is uncertain. In addition, the adhesion effect of the cells is not good. Repeated liquid addition steps in the microplate reader may cause cells to fall off the bottom of the plate. These uncertain factors also cause inaccurate and difficult to repeat results of FlexStation 3

Active compounds screened by the traditional calcium fluorescence high-throughput method are usually difficult to be effectively verified on the gold index patch clamp, indicating that the accuracy of the screening results using the traditional calcium fluorescence technology is not high, and there are a large number of false positive or false negative results.

The whole-cell patch clamp technique is not suitable for large-scale screening due to its high technical threshold and low throughput.

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