Hydrogel for bone repair, and preparation method thereof

A hydrogel and bone repair technology, applied in the field of biomedical engineering materials, can solve problems such as poor solubility and limit direct application, and achieve the effects of inhibiting bone resorption, good biocompatibility, and promoting bone formation

Pending Publication Date: 2020-05-01
HANGZHOU HUIBO SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan has poor solubility in neutral and alkaline media, which limits its direct application in the fields of medicine and biomedicine.

Method used

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  • Hydrogel for bone repair, and preparation method thereof
  • Hydrogel for bone repair, and preparation method thereof
  • Hydrogel for bone repair, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 carboxymethyl chitosan

[0034] Dissolve 2.0g of chitosan in 25.0mL of 50% NaOH, soak for 24h, and swell and alkalinize it with continuous ultrasound; crush the alkalized chitosan and transfer it to a beaker, stir it magnetically, and take 5g of chloroacetic acid Dissolve in 25mL of isopropanol, add drop by drop within 20min, react at room temperature for 8h, filter with suction to remove solvent; dissolve the filtrate in 100mL of water, adjust pH to 7 with 1M hydrochloric acid, then use 400mL of 90% ethanol Precipitate, filter with suction and wash with absolute ethanol three times, dry at 60°C to obtain carboxymethyl chitosan.

Embodiment 2

[0035] The preparation of embodiment 2 carboxymethyl chitosan-amorphous calcium phosphate (CMCh-ACP)

[0036] 10mM dipotassium hydrogen phosphate (K 2 HPO 4 ) solution was added dropwise to 20mM calcium chloride dihydrate (CaCl 2 2H 2 O) and carboxymethyl chitosan (CMCh, degree of deacetylation 90%, Santa Cruz Biotechnology, Inc.) in a premixed aqueous solution, stirred at 1000 rpm at room temperature to prepare CMCh-ACP hybrid nanoparticles. The concentrations of calcium chloride, potassium phosphate and CMCh in the final dispersion were 10 mM, 5 mM and 2 mg / mL, respectively. Isolate by centrifugation, lyophilize at -4°C, and store at -80°C until use.

Embodiment 3

[0038] A kind of CMCh-ACP / GelMA hydrogel, its preparation method is: take 2g CMCh-ACP and dissolve in 20mL distilled water, add the GelMA of 0.8g, then irradiate 50s with 3W 405nm ultraviolet light, obtain CMCh-ACP / GelMA gel glue.

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Abstract

The invention relates to a hydrogel for bone repair. The hydrogel is prepared from the following components in parts by weight: 1 to 3 parts of carboxymethyl chitosan-amorphous calcium phosphate, 0.5to 1 part of methacrylic anhydride gelatin and 0.1 to 0.2 part of dexamethasone. According to the invention, carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) are compounded to prepare a nano material, the nano material is mixed with methacrylic anhydride gelatin (GelMA), and a CMCh-ACP / GelMA hydrogel is formed under the condition of ultraviolet irradiation while dexamethasone (Dex) is loaded to slowly release, so that the problems of soft tissue edema, inflammation, serum and accidental bone formation possibly caused by sudden release of Dex are solved so as to promote boneformation; in the hydrogel, CMCh-ACP has an osteogenesis induction effect, induces the expression of osteogenesis regulatory factors and bone markers in MSCs in vitro, and inhibits the bone absorptionin a long-term ectopic osteogenesis process; and the hydrogel can serve as a novel bone tissue scaffold based on bone tissue cells so as to be further developed.

Description

technical field [0001] The invention relates to a hydrogel for bone repair and a preparation method thereof, belonging to the field of biomedical engineering materials. Background technique [0002] The treatment of large bone defects remains a thorny issue. Autologous bone grafting and allografting are the two main treatments for bone defects; however, both methods carry considerable risks for the patient, such as significant pain, fractures, nonunion and infection, growth factor deficiency . Effective bone tissue engineering can restore bone and skeletal function damaged by trauma and / or certain medical conditions. Bone is a complex tissue that functions through a coordinated interplay of cells, biomechanics, and biological factors. [0003] Bone tissue engineering holds promise for restoring bone and bone tissue function impaired by large segmental defects, severe fracture nonunion, and / or clinical conditions including cancer resection and bacterial infection. Approxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/28C08L5/08C08L89/00C08B37/08A61L27/12A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54
CPCC08J3/075C08J3/28C08B37/003A61L27/52A61L27/50A61L27/54A61L27/12A61L27/20A61L27/222C08J2305/08C08J2489/00A61L2430/02A61L2400/04A61L2400/12A61L2300/43A61L2300/602C08L5/08
Inventor 吴刚郭靖卢云宇吴丽勇
Owner HANGZHOU HUIBO SCI & TECH CO LTD
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