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Synbiotic micro-ecological preparation for pigs and preparation method and application of synbiotic micro-ecological preparation

A probiotic and synbiotic technology, applied in applications, animal feed, additional food elements, etc., can solve the problems of unguaranteed strain stability and unclear probiotic promotion effect, and achieve healthy and stable development, improve Pig growth performance and the effect of improving bacterial flora environment

Inactive Publication Date: 2020-05-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are a large number of synbiotic combination products on the market, but due to the specificity of different probiotics and prebiotics and the combination effect, the stability of the bacterial strains in the existing synbiotic microecological preparations cannot be guaranteed, and the effect of prebiotics on probiotics cannot be guaranteed. Facilitating effect is not clear

Method used

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  • Synbiotic micro-ecological preparation for pigs and preparation method and application of synbiotic micro-ecological preparation
  • Synbiotic micro-ecological preparation for pigs and preparation method and application of synbiotic micro-ecological preparation
  • Synbiotic micro-ecological preparation for pigs and preparation method and application of synbiotic micro-ecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and screening of lactic acid bacteria

[0028] 155 pig lactic acid bacteria were preliminarily screened from the intestinal mucosa, chyme, and feces of pigs at different growth stages (piglets, growing and finishing pigs, and sows), and 4 strains with strong acid-producing ability were screened out through routine biochemical identification and acid-producing ability. of lactic acid bacteria.

[0029] 2. Simulated gastrointestinal digestion

[0030] Artificial gastric juice: Add 3.0 g / L pepsin to sterile physiological saline with a pH of 2.5. After fully dissolving, filter and sterilize with a sterile microporous membrane (0.22 μm) to obtain artificial gastric juice for future use. Artificial intestinal juice: Add 1.0g / L trypsin and 1.8g / L bile salt to sterile saline with a pH of 8.0, fully dissolve, filter and sterilize for later use. The activated bacterial solution was inserted into the above-mentioned artificial gastric juice at an inoculum amount of ...

Embodiment 2

[0032] Example 2 Screening of the most suitable ratio of prebiotics and probiotics

[0033] By adding different proportions (1-3%) of inulin or fructooligosaccharides to the medium, the concentration that has the greatest impact on the acid production performance and growth rate of Lactobacillus plantarum L47 is selected as the added concentration of the synbiotic probiotics. It can be seen from Table 1 and Table 2 that when the addition amount is 3%, the lactic acid production and the number of viable bacteria of Lactobacillus plantarum L47 are the largest, so 3% is selected as the compatible addition content of prebiotics.

[0034] Table 1 Effects of different fructo-oligosaccharide addition ratios on Lactobacillus plantarum

[0035]

[0036] Table 2 Effects of different inulin addition ratios on Lactobacillus plantarum

[0037]

Embodiment 3

[0038] The preparation of embodiment 3 synbiotics probiotics

[0039] ① Lactobacillus plantarum was enriched by culturing in MRS medium at 37°C for 24 hours, centrifuged and resuspended to obtain a pure bacterial liquid. The composition of the MRS medium: peptone 10.0g, beef extract 10.0g, yeast extract 5.0 g, 2.0 g of diammonium hydrogen citrate, 20.0 g of glucose, 1.0 mL of Tween-80, 5.0 g of sodium acetate, 2.0 g of dipotassium hydrogen phosphate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, and 1000 mL of distilled water.

[0040] ② Add 3% inulin and (or) 3% fructo-oligosaccharide of the mass of the bacterial liquid to the Lactobacillus plantarum bacterial liquid with a viable count greater than 10.0 log cfu / mL, and add 5-10% of the bacterial liquid mass Cell protection agent (skimmed milk powder, glycerin or gelatin), freeze-dried after mixing. Wherein the degree of polymerization of fructo-oligosaccharides is DP=2-9, and the degree of polymerization of inul...

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Abstract

The invention discloses a synbiotic micro-ecological preparation for pigs and a preparation method and application of the synbiotic micro-ecological preparation for pigs. The preparation method of thesynbiotic micro-ecological preparation for pigs comprises the steps: mixing a Lactobacillus plantarum L47 bacterial solution with a viable count greater than 10.0 log cfu / mL, inulin with the mass of2-3% of the bacterial solution and / or 2-3% of fructo-oligose and a bacterium protection agent with the mass of 5-10% of the bacterial solution, and performing freeze drying so as to obtain the synbiotic micro-ecological preparation. The viable count of lactic acid bacteria can reach 9.4 log cfu / mL or above after growth of the synbiotic micro-ecological preparation is performed in vitro for 24 hours, and the concentration of lactic acid can reach 176.56 mM or above. The synbiotic micro-ecological preparation can be applied to production of monogastric animals, the intestinal flora environment of animals can be improved, and the growth of animals is promoted.

Description

technical field [0001] The invention belongs to the field of pig feed additives, and relates to a synbiotic microecological preparation for pigs and a preparation method and application thereof. Background technique [0002] In recent years, more and more probiotic and prebiotic products have been put into livestock production to improve the intestinal health and growth performance of livestock and poultry. As a kind of commonly used probiotics, lactic acid bacteria can inhibit the growth of pathogenic bacteria, promote the body’s absorption of nutrients, improve the body’s immunity and anti-diarrhea, etc. To achieve the above goals, probiotics must be able to pass through the gastrointestinal tract. Colonizes in the colon to function. The main obstacles it faces are the acidic conditions in the stomach and the environment of high concentrations (1-10 mM) of bile acids in the intestinal tract. Prebiotics refer to substances that cannot be digested by the enzymes secreted b...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/18A23K20/163
CPCA23K50/30A23K10/18A23K20/163
Inventor 杭苏琴朱晓峰张桢李英英崔雷鸿
Owner NANJING AGRICULTURAL UNIVERSITY
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