Extraction method of santalum album high-content essential oil tissue total RNA

Abstract

The invention discloses a method for extracting santalum album high-content essential oil tissue total RNA. The method for extracting the santalum album high-content essential oil tissue total RNA comprises the following steps: grinding santalum album wood tissue fluid in liquid nitrogen so as to obtain powder; adding the powder into Tris-HCl extraction liquid, carrying out freezing treatment at -80 DEG C, and carrying out centrifuging so as to obtain liquid supernatant; adding sodium acetate and isopropanol into the liquid supernatant so as to have RNA precipitated, carrying out centrifuging,and dissolving the precipitates by using a TE buffer solution; adding cetyltrimethylammonium bromide into the dissolved precipitates, carrying out uniform mixing, carrying out extraction by using chloroform / isoamyl alcohol, and centrifuging the extract so as to obtain liquid supernatant; and then, adding lithium chloride into the liquid supernatant so as to carry out RNA precipitation overnight, centrifuging the precipitated RNA, and washing the centrifuged RNA by using ethanol so as to obtain the total RNA. Being tested by using Nanodrop, the santalum album wood tissue total RNA extractedby the method disclosed by the invention has an OD<260> / OD<280> ratio of 2.0 or above, an OD<260> / OD<230> ratio of 2.3 or above, an RNA concentration in aqueous solution of 800.0 ng / [mu]L or more,and RIN higher than 7.5-1; and 1.0% denaturing agarose gel electrophoresis shows that the RNA strip is clear and good in integrity, so that the santalum album wood tissue total RNA has the advantagesof being high in quality and high in concentration.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for extracting total RNA from tissues with high content of sandalwood essential oil. Background technique [0002] Sandalwood (Santalum album L.) is a semi-parasitic evergreen tree of the genus Santalum in the family Santalaceae. It is a valuable and rare plant, mainly distributed in India, Indonesia, Australia and some islands in the Pacific Ocean. Humans have used it for thousands of years, and it is especially famous because its wood can be used for carving and refining sandalwood oil, known as "green gold". There is no native distribution of sandalwood in China, and its finished or semi-finished products have been imported from abroad in the past. In 1962, the South China Botanical Garden of the Chinese Academy of Sciences introduced sandalwood seeds from Indonesia to the mainland for the first time and succeeded in breeding. Later, it also successfully ...

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Problems solved by technology

[0005] Based on the wood tissue of sandalwood with high content of essential oil, polysaccharide, polyphenol and other secondary metabolites, the present invention develops a method for extracting total RNA from sandalwood wood tissue, which solves the difficulty in extracting RNA from sandalwood wood in the past , low quality, low output, etc.

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