Novel drug-loaded nano hydrogel, and preparation method and application thereof
A nano-hydrogel, drug-carrying technology, applied in pharmaceutical formulations, anti-tumor drugs, drug combinations, etc., can solve the problems of lack of versatility, cumbersome, and harsh processes, and achieve improved biocompatibility and releaseability. Control, raw material environment-friendly effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0035] The invention provides a preparation method of a novel drug-loaded nano-hydrogel, comprising the following steps:
[0036] (1) Adjust the pH value of the mixed solution of cadmium chloride and L-cysteine to 11.0, add sodium borohydride, sodium tellurite and ssDNA1 successively and heat to 95° C., then add sodium selenium hydride solution and continue heating for 2 hours, Get the DNA-QDs solution, wash with absolute ethanol, centrifuge, add PBS buffer solution to dissolve, and obtain CdTeSe quantum dots after purification and drying; the ssDNA1 is modified with an amino group on the base at the 5' end, and the six bases in the chain structure The P=O bond in the phosphate skeleton is sequentially modified into a P=S bond;
[0037] (2) After mixing the CMC solution with NHS and EDC, sonicate for 5 minutes, and after standing for 15 minutes, an activated CMC solution is obtained;
[0038] (3) Span80 and Tween80 are mixed and then ultrasonicated to obtain a Span+Tween em...
Embodiment 1
[0056] as per figure 1 The process shown in the preparation of novel drug-loaded nano-hydrogels:
[0057] S1. Design single-stranded DNA:
[0058] ssDNA1 modifies an amino group (-NH2) on the base at the 5' end, and the P=O bond in the phosphate backbone of the six bases in the chain structure is sequentially modified into a P=S bond;
[0059] ssDNA2 modifies an amino group (-NH2) on the base at the 5' end, and the rest are not modified;
[0060] ssDNA3 does not make any modifications, respectively:
[0061] ssDNA1:5'NH 2 -SEQ ID NO.1-3', the sequence of SEQ ID NO.1 is GGTGGTGGTGGTTGTG*G*T*G*G*T*GGTGG TAGCTTATC;
[0062] ssDNA2:5'NH 2 -SEQ ID NO.2-3 ', the sequence of SEQ ID NO.2 is
[0063] ssDNA3:5'-SEQ ID NO.3-3', the sequence of SEQ ID NO.3 is
[0064] S2. Preparation of DNA functionalized CdTeSe quantum dots:
[0065] Weigh 0.024mmol of anhydrous cadmium chloride and 0.0288mmol of L-Cys, and add them into a 50mL three-necked flask in turn, and pour 30mL of u...
Embodiment 2
[0074] S1, S2, S3 steps are the same as embodiment 1
[0075] Preparation of S4, Span+Tween emulsion
[0076] The mass ratio of Span80 and Tween80 is 5:5, the average mass is 0.522g, and the volume of n-hexane in the oil phase is 3mL. Weigh Span80 and Tween80 respectively and add them to a 5mL centrifuge tube, then add 3mL of n-hexane into it, and ultrasonically disperse them evenly for 2min.
[0077] S5, with embodiment 1. The TEM images of its macroscopic (main image) and single gel (upper left small image) morphology are shown in Figure 2b Shown; The fluorescence spectrum of the prepared NHY / Cys-(DOX, QDs) under 477nm fluorescence excitation is as follows Figure 3b shown
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More