Sensitization blocking buffer solution and application thereof

Abstract

The invention belongs to the technical field of diagnostic reagents, and discloses a sensitization blocking buffer solution containing a buffer solution, 0.2%-0.2% 10% (v / v) ethanol; 0.1%-5%(w / v)PEG6000, 0.01%-0.5% (w / v) of sodium carboxymethyl cellulose (M.W.90000), 0.005%-0.25% (w / v) of poloxamer 188, 0.05%-5% (w / v) of glycine betaine, 0.1% (v / v) of Tween20 and 0.045% (v / v) of ProClin 300. The buffer solution is a phosphate buffer salt solution with the pH value of 5.8-8.0, an HEPES buffer salt solution with the pH value of 6.8-8.2, a PIPES buffer salt solution with the pH value of 6.1-7.5,an MOPS buffer salt solution with the pH value of 6.5-7.9 and a Tris buffer salt solution with the pH value of 7.1-9.0. The sensitization blocking buffer solution and a to-be-detected sample are incubated at the same time to participate in reaction; the background of a negative sample of a detection result is remarkably reduced, the signal value of a positive sample detection result is increased,the ratio of the positive sample signal value to the negative sample signal value of the detection result is increased under the dual action of blocking non-specific and sensibilization specific reactions, and the detection sensitivity and specificity are improved. The sensitization blocking buffer solution can be used for preparing a helicobacter pylori urine antibody detection kit.

Description

technical field [0001] The invention belongs to the technical field of diagnostic reagents, and in particular relates to a sensitization blocking buffer, a kit and an application. Background technique [0002] Immunodiagnosis (immunodiagnosis) is the application of the theory, technology and methods of immunology to diagnose various diseases and determine the immune status. Immunodiagnostic reagents are widely used in hospitals, third-party testing, blood stations, and physical examination centers. They are mainly used for infectious disease detection, infectious disease detection, tumor detection, and metabolic marker detection. Among them, immunodiagnosis includes radioimmunoassay, enzyme-linked immunoassay, immunochromatography, chemiluminescence, etc. Immunodiagnosis has the following characteristics: ①Strong specificity, try to avoid cross-reaction and false positive, so as to ensure the accuracy of diagnosis; ②High sensitivity, can detect trace reaction substances and...

AI Technical Summary

Problems solved by technology

At present, no Helicobacter pylori antibody detection kit has been used clinically. One of the main reasons is that the sensitivity of conventional detection methods cannot meet the requirements due to the low antibody content in urine, and the presence of other interfering substances is easy to form cross-interference, which is harmful to the test. influence of specificity

Images