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Method for preparing high-efficiency bacteriostatic agent from reed leaf extract

The technology of extract and bacteriostatic agent is applied in the field of preparation of biosynthetic nano-silver bacteriostatic agent by using reed extract, which can solve problems such as differences in physical and chemical properties and biological activity of nano-silver, and achieves low production cost, mild reaction and environmental protection. , the effect of broad antibacterial spectrum

Active Publication Date: 2020-06-19
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although people have successfully prepared nano-silver from plant extracts such as ginger, orange peel, mango peel, blueberry leaf, and cabbage, studies have shown that there are large differences in the physical and chemical properties and biological activities of nano-silver prepared from different biological materials.

Method used

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  • Method for preparing high-efficiency bacteriostatic agent from reed leaf extract
  • Method for preparing high-efficiency bacteriostatic agent from reed leaf extract
  • Method for preparing high-efficiency bacteriostatic agent from reed leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of preparation technology of the nano-silver antibacterial agent that utilizes reed leaf extract biosynthesis of the present embodiment, concrete steps are as follows:

[0027] (1) Material preparation:

[0028] The reed leaves were collected, rinsed with tap water, washed three times with double distilled water, and dried at 60°C to constant weight to obtain dried reed leaves.

[0029] (2) preparation of reed extract:

[0030] Weigh 1.0 g of the above-mentioned reed leaves, cut the leaves into pieces, add 100 mL of double distilled water, heat and reflux at 90°C for 3 hours, cool the prepared solution naturally, filter with suction, centrifuge at 8000 rpm for 10 minutes, and take the supernatant to obtain the reed leaf extract liquid.

[0031] (3) One-step biosynthesis of silver nanoparticles using reed leaf extract as a reducing agent:

[0032] Take 5 mL of the reed leaf extract above, add it to 14 mL of double-distilled water, then add 1 mL of 0.1 M silver ...

Embodiment 2

[0034] A kind of preparation technology of the nano-silver bacteriostatic agent that utilizes reed leaf extract biosynthesis of the present embodiment, concrete steps are as follows:

[0035] (1) Material preparation:

[0036] The reed leaves were collected, rinsed with tap water, washed three times with double distilled water, and dried at 60°C to constant weight to obtain dried reed leaves.

[0037] (2) preparation of reed extract:

[0038] Weigh 5.0 g of the above-mentioned reed leaves, cut the leaves into pieces, add 100 mL of 70% ethanol, heat and reflux at 70 ° C for 1 hour, cool the prepared solution naturally, filter with suction, centrifuge at 8000 rpm for 10 minutes, and take the supernatant to obtain the reed leaf extract .

[0039] (3) One-step biosynthesis of silver nanoparticles using reed leaf extract as a reducing agent:

[0040] Take 1 mL of the above-mentioned reed leaf extract, add it to 18 mL double-distilled water, then add 200 μL of 0.1M silver nitrate...

Embodiment 3

[0042] Characterization of reed biosynthetic nano-silver bacteriostatic agent by UV-Vis spectrophotometer:

[0043] The maximum absorption spectrum of the biosynthesized nano-silver solution synthesized in Example 2 was detected by an ultraviolet-visible spectrophotometer, and the reed leaf extract was used as a control, and the full wavelength range of 300-800nm ​​was scanned. From attached figure 1 It can be seen that the reed biosynthesized nano-silver has the maximum absorption peak near 419nm, but the reed leaf extract has no absorption peak. The surface plasmon resonance (SPR) absorption peak range of nano-silver is within 400-500nm, so it can be inferred that the reed leaf extract will successfully mediate the production of nano-silver.

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Abstract

The invention relates to a preparation technology of a novel bacteriostatic agent, in particular to a method for biosynthesizing a nano-silver bacteriostatic agent from a reed extract. The method comprises the following steps: 1, collecting reed leaves, cleaning and drying to constant weight, 2, cutting the dried reed leaves into pieces, putting the reed leaves and an extracting agent into a reflux device for heating reflux, naturally cooling and filtering a product obtained after heating reflux, then carrying out centrifugal treatment, taking a supernatant to obtain a reed leaf extracting solution which is light yellow, and storing for later use, and 3, mixing the reed leaf extracting solution with double distilled water to obtain a mixed solution, adding a silver nitrate solution, uniformly stirring, carrying out heating reflux treatment, and stopping heating reflux when the solution becomes golden yellow, thereby obtaining the nano-silver bacteriostatic agent. The nano-silver bacteriostatic agent is rich in raw material resources, low in cost and environmentally friendly, rich antioxidant substances in reed leaves are used as a reducing agent, and the synthesized nano-silver bacteriostatic agent has remarkable bacteriostatic activity and stability.

Description

technical field [0001] The invention relates to a preparation technology of a novel bacteriostatic agent, in particular to a preparation method for biosynthesizing a nano-silver bacteriostatic agent by using a reed extract. Background technique [0002] Phragmites communis Trin. is a perennial aquatic herbaceous plant belonging to the genus Phragmites communis Trin. Phragmites reeds are mostly born in wetlands and shallow waters. They have strong reproductive ability, large biomass, and strong environmental adaptability. They play an important role in water and soil conservation, saline-alkali land management, and ecological environment protection. Reed is rich in fiber content and can be used for various purposes such as civil building materials, weaving, textiles, papermaking, and feed processing. In addition, reed also has important medicinal value and health care function. Its roots, stems, and leaves are rich in arabinose, xylose, glucose, starch, rutin, scutellarin, h...

Claims

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Application Information

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IPC IPC(8): A01N65/44A01N59/16A01P1/00B22F9/24B82Y40/00
CPCA01N59/16A01N65/00A01N65/44B22F9/24B82Y40/00
Inventor 王磊宿红艳吴凡林肖雨晴马欣冉
Owner LUDONG UNIVERSITY
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