A kind of magnetic biological microcapsule, preparation method and application thereof
A microcapsule and biological technology, applied in biochemical equipment and methods, microcapsule preparations, microbial-based methods, etc., can solve the problems of slow repair speed of plant methods, high cost of physical and chemical methods, and difficulty in popularization and application. Migration of free heavy metals, no secondary pollution, good effect
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Embodiment 1
[0027] A preparation method of magnetic biomicrocapsules, the method steps are as follows:
[0028] (1) get 2mol / L calcium chloride solution and 2mol / L sodium bicarbonate solution of the amount of equal substance respectively, add polystyrene sodium sulfonate in calcium chloride solution according to the ratio of 10g / L, according to 10g / L Add NdFeB in the ratio of NdFeB, add equal mass of sodium polystyrene sulfonate and NdFeB to the sodium bicarbonate solution, stir at a speed of 1200rpm, and stir for 1 hour; slowly add the calcium chloride solution to the NaHCO3 solution dropwise , the reaction temperature is 50°C, and the standing time is 2 hours; centrifugation is used, the sediment is washed with ethanol solution, and porous calcium carbonate microcapsules are obtained after drying. The calcium carbonate microcapsules are spherical porous structures with a diameter of 50-65 μm.
[0029] (2) Bacillus colloidus is inoculated in the culture medium solution after sterilizatio...
Embodiment 2
[0034] A preparation method of magnetic biomicrocapsules, the method steps are as follows:
[0035] (1) get 2mol / L calcium chloride solution and 2mol / L sodium bicarbonate solution of the amount of equal substance respectively, add polystyrene sodium sulfonate in the calcium chloride solution according to the ratio of 5g / L, according to 1g / L Add rubidium-iron-boron in proportion to the sodium bicarbonate solution, add polystyrene sodium sulfonate and rubidium-iron-boron of equal mass, stirring speed 1000rpm, stirring time 1.5 hours; slowly drop calcium chloride solution into the sodium bicarbonate solution In the process, the reaction temperature is 15°C, and the standing time is 1.5 hours; centrifugation is used, the sediment is washed with ethanol solution, and porous calcium carbonate microcapsules are obtained after drying. The calcium carbonate microcapsules are spherical porous structures with a diameter of 85-100 μm.
[0036] (2) Bacillus colloidus is inoculated in the c...
Embodiment 3
[0041] (1) Add 10g / L organic template polystyrene sodium sulfonate, 5g / L magnetic particles NdFeB to calcium chloride solution and sodium bicarbonate solution respectively, stirring speed 800rpm, stirring time 1 hour; Calcium chloride The solution is slowly added dropwise into the sodium bicarbonate solution, the reaction temperature is 35°C, and the standing time is 2 hours; the sediment is washed by centrifugation and ethanol solution, and the porous calcium carbonate microcapsules are obtained after drying. The calcium carbonate microcapsules are 62 in diameter. -73μm spherical porous structure.
[0042] (2) Bacillus colloidus is inoculated in the culture medium solution after sterilization, every liter of culture medium contains sucrose 8g, Na 2 HPO 4 12H 2 O 2g, MgSO 4 0.4g, CaCO 3 0.5g, KCl 0.1g, (NH 4 ) 2 SO 4 0.4g, and control the pH to 7, shake culture at 35°C for 48 hours to obtain the bacterial liquid containing Bacillus colioids, add manganese chloride, a...
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