A method for producing 1,3-propanediol by fermentation of glycerol by microorganisms

A technology of microorganisms and glycerin, applied in the field of bioengineering, can solve the problems of low conversion rate and high production cost, and achieve the effect of increasing conversion rate and reducing production cost
CN111394396BActive Publication Date: 2022-04-08镇江百泰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
镇江百泰生物科技有限公司
Publication Date
2022-04-08

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Abstract

The invention provides a method for producing 1,3-propanediol by fermentation of glycerol in a microbial co-cultivation system, by successfully constructing recombinant Escherichia coli Rosseta-dhaB1dhaB2 and Rosseta-dhaT, co-cultivating the above-mentioned recombinant Escherichia coli for seed culture and fermentation culture , wherein the recombinant Escherichia coli Rosseta-dhaB1dhaB2 realizes the utilization of glycerol to produce the intermediate metabolite 3-hydroxypropionaldehyde (3-HPA), and the recombinant Escherichia coli Rosseta-dhaT catalyzes 3-HPA to produce 1,3-propanediol. During the fermentation process, press Inoculate in the fermentation medium at a ratio of 1:1.5, add 60g / L substrate glycerol and 10g / L cosubstrate glucose. The method of the present invention improves the conversion rate of glycerol and the output of 1,3-propanediol, and the present invention adopts Clostridium butyricum containing independent coenzyme B 12 Glycerol dehydratase, which reduces the production cost.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for microorganisms to ferment and produce 1,3-propanediol using glycerol. Background technique

[0002] Crude glycerin is one of the main by-products in the production of biodiesel. Every 10kg of biodiesel produced will produce 1kg of crude glycerin. According to reports, it is expected to reach more than 5.87 billion pounds in 2020. If crude glycerin cannot be used and processed in a timely and effective manner, It may become a new source of pollution. Therefore, using glycerol to produce high value-added products and improving the conversion rate of glycerol is a hot spot in current research.

[0003] 1,3-Propanediol (1,3-PD), as a good solvent, antifreeze and protective agent, is widely used in the fields of food, medicine, cosmetics and chemical industry. In the field of food, 1,3-propanediol is easy to dissolve food additives that are difficult ...

Claims

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