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A compound acellular matrix injection for treating femoral head necrosis and using method

A technology of acellular matrix and femoral head necrosis, which is applied in drug combinations, pharmaceutical formulas, bone diseases, etc., can solve the problems of inability to fix mesenchymal stem cells, inability to provide a regenerative microenvironment, and unfavorable renal excretion, so as to avoid pioneering surgery risk, speed up the full recovery time, and the effect of no toxic side effects

Active Publication Date: 2022-04-15
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The patent (CN201910178248.X) discloses a preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection in the treatment of ischemic femoral head necrosis. The cartilage differentiation inducer of the injection includes glucose isoflavone, bone Formation protein-2, ascorbic acid, dexamethasone, and sodium β-glycerophosphate, these inducers cannot fix mesenchymal stem cells and cannot provide a regenerative microenvironment, and stem cells are easily lost in the body
The patent (CN201010152461.2) discloses a cell injection for treating bone damage and its preparation method. The gel is mainly poloxamer gel, which belongs to high molecular polymer and is not conducive to renal excretion.
[0008] At present, there are no relevant reports on the composite regenerative materials of acellular matrix fluid, bone marrow mesenchymal stem cells and angelica parasitic fluid to construct a tissue repair microenvironment for the repair of femoral head necrosis tissue

Method used

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  • A compound acellular matrix injection for treating femoral head necrosis and using method
  • A compound acellular matrix injection for treating femoral head necrosis and using method
  • A compound acellular matrix injection for treating femoral head necrosis and using method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the preparation method of composite acellular matrix injection

[0054] Preparation method of acellular matrix solution: (1) Firstly weigh 500g of porcine Achilles tendon tissue, wash it with PBS and other pretreatments, inactivate the virus and crush it; (2) Add 2L of 10% sodium chloride solution for soaking Shaking table for 2 hours, 30°C / 200rpm; (3) filter, wash the tissue with a large amount of purified water 3 times, 30min each time, shaker for 10min, 30°C / 200rpm; (4) use 2L 3% sodium carbonate Soak the solution for 3 hours to degrease, wash with purified water 3 times: 10min / time, 30℃ / 200rpm; (5) Soak the shaker with 5L 4% trypsin for 3 hours, wash with purified water 3 times: 10min / time, 30℃ / 200rpm; ( 6) Degrade the nucleic acid components in the cells by nuclease, wash thoroughly to remove the detergent to obtain the acellular matrix, and freeze-dry to obtain the acellular matrix powder; (7) prepare pH=2 HCl solution, 10×PBS solution, 1×PBS soluti...

Embodiment 2

[0058] Example 2. Take 1.2 parts of acellular matrix liquid, 1 part of bone marrow mesenchymal stem cell liquid and 0.8 part of Angelica Jisheng injection in the composite acellular matrix injection in Example 1, and mix them according to the proportion during the operation. Fill into a 10ml syringe and compound into an injectable mixture.

Embodiment 3

[0059] Example 3. Take 0.8 parts of acellular matrix liquid, 1 part of bone marrow mesenchymal stem cell liquid and 1.2 parts of Angelica Jisheng injection in the composite acellular matrix injection in Example 1, and mix them according to the proportion during the operation. Fill into a 10ml syringe and compound into an injectable mixture.

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Abstract

The invention discloses a composite acellular matrix injection, which is used as a repair material for femoral head necrosis for clinical treatment. The composite acellular matrix injection is composed of biological acellular matrix sol, bone marrow mesenchymal stem cells and angelica parasitic fluid, and is injected into the hip joint between the femoral head and the acetabulum during the operation to provide a microenvironment for tissue repair , promote the proliferation and differentiation of bone marrow mesenchymal stem cells into osteoblasts, induce new blood vessel formation and new bone formation, and have anti-inflammatory and analgesic effects, and can be used for the treatment of early and mid-term non-traumatic necrosis of the femoral head.

Description

technical field [0001] The invention relates to a preparation method of a composite acellular matrix injection and its therapeutic application in femoral head necrosis, belonging to the field of medical biomaterials and tissue engineering repair. Background technique [0002] The pathogenic factors of non-traumatic femoral head necrosis are mainly divided into two types: chronic alcoholism and abuse of hormone drugs. Alcohol and hormones hinder the blood circulation around the femoral head, causing insufficient blood supply, resulting in necrosis of the femoral head, fracture of trabeculae, collapse of the femoral head, and paralysis of the patient in severe cases. Femoral head necrosis is divided into four stages: , The period is the early stage of subsidence; The period is the period of femoral head collapse, In the early stage, the femoral head collapses and develops into arthritis, which can be treated in the early and middle stages, and artificial hip replacemen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/08A61K36/232A61K47/46A61P19/08A61L27/38A61L27/36A61L27/50A61L27/54A61L27/58A61K35/28
CPCA61K36/232A61K36/185A61K35/28A61K9/0019A61K9/08A61K47/46A61P19/08A61L27/3834A61L27/3847A61L27/3895A61L27/3637A61L27/3633A61L27/3687A61L27/3691A61L27/365A61L27/50A61L27/54A61L27/58A61L2300/602A61L2430/40A61L2300/412A61L2430/02A61L2300/30A61L2300/41A61L2300/402A61L2400/06A61K2300/00
Inventor 高秀伟高秀岩雷蕾郑红霞
Owner 山东隽秀生物科技股份有限公司
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