Pseudomonas-anode photosynthetic solar fuel cell system and preparation method and application thereof
A fuel cell system and technology of Pseudomonas, applied in the field of plant-microbial fuel cells, can solve the problems of high inactivation rate of a single strain, low possibility of application, low operability, etc., and achieve high energy conversion efficiency , the reaction conditions are mild, and the effect of increasing the output power
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Embodiment 1
[0066] 1. Testing the relative abundance of microorganisms at the anode of the anode photosynthetic solar fuel cells (APFSCs) system under different pollution conditions
[0067] The specific implementation method of this embodiment is to find out the electrochemically active microorganisms with a large proportion and a large contribution by detecting and analyzing the operation of the anode photosynthetic solar fuel cell (APFSCs) system and the anode microbial community under different pollution conditions. Thereby, the electrochemically active microbial strains with stronger performance are screened out, and the reliability of the added strains of the present invention is verified. For the preparation method of the anode photosynthetic solar fuel cells (APFSCs) system described in this embodiment, see the same day application for the invention patent "a kind of anode Photosynthetic Solar Fuel Cell System".
[0068] Grouping experiments were carried out for different pollutio...
Embodiment 2
[0099] The materials required in this example are: Pseudomonas aeruginosa freeze-dried strains, nutrient broth liquid medium, sterile glycerin, normal saline, nutrient agar plates, and the Pseudomonas aeruginosa strains were purchased from Wuhan Water Country Environmental Science and Technology Co., Ltd.
[0100] 1. Strain cultivation steps:
[0101] (1) Recovery and rejuvenation of strains: use a straw to draw 0.3ml of nutrient broth liquid medium into the bottom of the bottle and blow it to make a bacterial suspension, suck out all the bacterial suspension, and inoculate the medium; dry Pseudomonas aeruginosa The strains were transferred to conical flasks containing 100ml nutrient broth liquid medium for recovery (the first generation), and cultured at a constant temperature in a shaker at 35°C for 18 hours to obtain the first generation culture; take 1ml of the first generation culture The material was inoculated into an Erlenmeyer flask equipped with 100ml of nutrient br...
Embodiment 3
[0105] This embodiment provides a Pseudomonas-anode photosynthetic solar fuel cell system, including 1-anode, 2-cathode, 3-typha aquatic plants, 4-resistance, 5-data collector, 6-water body, 7-soil and Pseudomonas aeruginosa. See the specific device figure 1 .
[0106] The preparation method of the Pseudomonas-anode photosynthetic solar fuel cell system of this embodiment comprises the following steps:
[0107] (1) Substrate production: take the soil of the riverside wetland after air-drying, grind and sieve to obtain the substrate for cultivating plants;
[0108] (2) plant material and propagation and cultivation: select cattail aquatic plants and place them in a container filled with sandy soil for cultivation;
[0109] (3) Construct a single-chamber battery anode containing microorganisms: use an opaque and non-conductive container as a reactor, use foamed nickel materials for both the anode and cathode, and use biomass porous carbon to anode-modify the foamed nickel ele...
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