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Screening method and application of chemical inducer for improving oil production of green algae

A chemical inducer and screening method technology, applied in the field of chemical inducer screening, can solve the problems of time-consuming, laborious and low detection efficiency

Active Publication Date: 2020-07-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These assay methods have the disadvantages of time-consuming and labor-intensive, low detection efficiency, etc.

Method used

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  • Screening method and application of chemical inducer for improving oil production of green algae
  • Screening method and application of chemical inducer for improving oil production of green algae
  • Screening method and application of chemical inducer for improving oil production of green algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1 Preliminary screening experiment of the influence of various chemical inducers on the autofluorescence of Chlorella zoffii

[0114] In this example, a variety of different chemical inducers combined with high light and nitrogen-free stress conditions were used to induce stress culture of Chromochloris zofingiensis (Chromochloris zofingiensis, American Culture Collection Center ATCC 30412) cells, and then flow cytometry was used to determine the adjuvant The average fluorescence intensity value of the Chlorella zoffensis cells in the FL3 channel (>670nm), through rapid detection and evaluation, and preliminary screening to determine the chemical inducer and its appropriate concentration that contribute to the increase in the fluorescence intensity value of Chlorophyta Zoffeae cells.

[0115] 1.1 Strain activation and seed solution preparation

[0116] Transfer the Zoff color green algae C. zofingiensis strains preserved in the laboratory to the slant equipped w...

Embodiment 2

[0139] The impact of embodiment 2 different chemical inducers on Zoff's green algae biomass and total lipid accumulation

[0140] Considering that different induction stress conditions are used for the cultivation of Chlorella zoffensis, the ratio and content of chlorophyll accumulated in the cells will be quite different, which will affect the stability of FCM rapid detection and evaluation. Therefore, in order to improve the accuracy of the initial screening, this embodiment is based on the preliminary screening results of FCM rapid assessment, combined with verification experiments to finally determine the most suitable chemical inducer and its optimum concentration, that is, firstly, the initial screening determines the concentration of each chemical inducer. Then use the same induction conditions to re-do the culture verification experiment of Zoff color green algae cells. After obtaining enough algae cells, use the traditional quantitative method of organic solvent extrac...

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Abstract

The invention discloses a screening method and application of a chemical inducer for improving oil production of green algae. The method comprises the following steps of S1, inoculating activated green alga cells into a nitrogen-free sugar-containing culture medium, dividing the activated green alga cells into n parts, then adding the chemical inducer with a concentration gradient for induction culture to sequentially obtain inducer group green alga cells, and meanwhile, obtaining control group green alga cells by taking the condition that no chemical inducer is added as a control; S2, measuring the average fluorescence intensity of chlorophyll in the inducer group green alga cells and the control group green alga cells by adopting a flow cytometer; and S3, judging whether the chemical inducer can increase the oil yield in the green alga cells or not according to the average fluorescence intensity of the chlorophyll in the inducer group green alga cells and the control group green algacells, and accordingly screening out the optimal induction concentration. According to the method disclosed by the invention, the chemical inducer capable of effectively improving the oil yield of the green algae can be screened out, and then the chemical inducer can be used for culturing the green alga cells to produce biodiesel raw oil and / or edible alga oil.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a screening method and application of a chemical inducer for improving oil production of green algae. Background technique [0002] Microalgae is a general term for a large class of single-celled lower plants. It has the dual characteristics of plants and microorganisms. Special species can accumulate a large amount of oil under the conditions of nitrogen deficiency and high carbon-to-nitrogen ratio. It is an algae-based biofuel and algae-based food. The high-quality raw material of oil, its biomass and high-yield fermentation technology of oil (total fat and fatty acid) have always been a research hotspot in this field. Chromochloris zofingiensis is a unicellular green alga with a fast growth rate capable of heterotrophic high cell density fermentation using organic carbon sources under dark conditions while accumulating carotenoids intracellularly at high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38G01N33/50G01N21/64C12R1/89
CPCC12N1/12C12N1/38G01N33/50G01N21/6486
Inventor 魏东陈俊辉
Owner SOUTH CHINA UNIV OF TECH
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