SARS-CoV-2 antibody detection method

An antibody detection, sars-cov-2 technology, applied in the field of SARS-CoV-2 antibody detection, can solve the problem of high homology and achieve important application prospects and value
CN111474345APending Publication Date: 2020-07-31BEIJING BIOSYNTHESIS BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING BIOSYNTHESIS BIOTECH
Publication Date
2020-07-31

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Abstract

The invention discloses an SARS-CoV-2 antibody detection method. The SARS-CoV-2 antibody detection method comprises the following steps: carrying out horseradish peroxidase labeling on recombinant SARS-CoV-2Nucleocapsid protein, meanwhile, selecting an anti-SARS-CoV-2 antibody or a dominant antibody to coat the enzyme-linked plate and sealing; according to a competitive ELISA principle, simultaneously adding a to-be-detected sample and an enzyme-labeled antigen into a test hole of an enzyme-linked plate, performing incubation at room temperature, thoroughly washing the plate, then adding a substrate TMB, performing color development in a dark place, finally, terminating the reaction, measuring a light absorption value at 450 nm, and evaluating the titer of the antibody in the sample by calculating the antibody inhibition rate. According to the method disclosed by the invention, relatively conservative N protein in SARS-CoV-2 and a specific antibody of the N protein are selected as rawmaterials. Compared with a traditional antibody detection method, the detection of the Anti-SARS-CoV-2 specific antibody is completed more quickly, more conveniently and more sensitively, and the method has specificity, sensitivity, accuracy and precision and has important application prospect and value.
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Description

technical field

[0001] The invention relates to a SARS-CoV-2 antibody detection method, belonging to the technical field of antibody detection. Background technique

[0002] SARS-CoV-2 belongs to the genus β of the Coronaviridae family. A scientific research team found that the sequence homology of the entire genome of SARS-CoV-2 with SARS-CoV (Severe Acute Respiratory Syndrome) is about 76%, and the sequence homology with bat coronavirus BatCoVRaTG13 in horseshoe bats is as high as 96% At the same time, the team confirmed that the receptors for SARS-CoV-2 and SARS-CoV to enter cells are both ACE2 by comparing their seven conserved non-structural proteins.

[0003] The structure of coronavirus consists of a double-layer lipid envelope, including Spike glycoprotein (S), envelope protein (Envelope protein, E), membrane protein (Membrane glycoprotein, M) and nucleocapsid protein (Nucleocapsid protein, N). Among them, S protein and N protein are the most important target pr...

Claims

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