2, 4-dichlorophenoxyacetic acid on-site quantitative detection platform based on integration of target response type 3D printing model and smart phone

A 3D printing and smart phone technology, applied in the field of biosensors, can solve the problems of cumbersome test methods, poor test stability, inconvenient portability, etc., and achieve the effects of low cost, good stability, and improved selectivity

Active Publication Date: 2020-08-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a 2D-based 3D printing model integrated with a smart phone based on a target responsive 3D printing model in order to overcome the problems of poor test stability, cumbersome test methods, inconvenient portability and low selectivity in the existing pesticide detection technology. , 4-D on-site quantitative detection platform (abbreviated as 3D printing model-smart phone detection platform), this platform can detect the content of 2,4-D accurately, easily and with high selectivity

Method used

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  • 2, 4-dichlorophenoxyacetic acid on-site quantitative detection platform based on integration of target response type 3D printing model and smart phone
  • 2, 4-dichlorophenoxyacetic acid on-site quantitative detection platform based on integration of target response type 3D printing model and smart phone
  • 2, 4-dichlorophenoxyacetic acid on-site quantitative detection platform based on integration of target response type 3D printing model and smart phone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Preparation of CDs / CoOOH composite material

[0038] First, add 1.0g of citric acid and 2.0g of urea into 10.0mL of N,N-dimethylformamide solution (≥99.9%), stir for 10min, react at a constant temperature of 160°C for 6h, and wash with alkali after cooling ( 20mL, 1.2mol L -1 Sodium hydroxide aqueous solution), pickling (25mL, 5wt% hydrochloric acid solution), centrifugation after washing, and freeze-drying to obtain CDs; secondly, 3.0mL, 1.0mol L -1 Sodium hydroxide aqueous solution and 10.0mL, 10.0mmol L -1 500 μL, 0.9mol L -1 The aqueous solution of sodium hypochlorite was added and ultrasonicated for 15 minutes, and the aqueous solution of CoOOH nanosheets was obtained after centrifugation. 5.0mL 0.3mg mL -1 CDs aqueous solution and 5.0mL CoOOH nanosheet aqueous solution (0.025mg mL -1 ) were mixed and stirred for 10 min, and centrifuged to obtain an aqueous solution of CDs / CoOOH composite material.

Embodiment 2

[0039] Example 2: Acquisition of Smartphone Assisted Images

[0040] 50 μL of 2,4-D aqueous solution (1.0 mg L -1 ) and 50μL ALP aqueous solution (7.0U L -1 ) were mixed and incubated at 37°C for 30 min to achieve the inhibition of ALP activity by 2,4-D; subsequently, 120 μL of L-ascorbic acid-2-phosphate trisodium salt solution (100 μmol L -1 ) and 100 μL Tris-HCl buffer solution with a pH of 8.0, after incubating at 37°C for 10 minutes, the aqueous solution (160 μL) of CDs / CoOOH composite material prepared in Example 1 was added and mixed evenly; after equilibrating at 25°C for 10 minutes, the ratio Put the color dish into the 3D printing model, and obtain the corresponding red fluorescent picture under the concentration of 2,4-D in this example under the 532nm laser. Under the 2,4-D concentration in this example, the grayscale value of the image corresponding to the sample read by the mobile phone is 42.57.

Embodiment 3

[0041] Embodiment 3: Application of APP to 2,4-D detection

[0042] According to the method of Example 2, different concentrations of 2,4-D (0.1, 0.5, 1.0, 2.0, 5.0, 10.0, 12.0 and 15.0mg L -1 , the gray values ​​corresponding to different concentrations are 46.46, 44.11, 42.57, 40.86, 35.34, 28.93, 22.20, 18.62, 12.29. It can be observed with the naked eye that with the increase of 2,4-D, the color of the solution in the cuvette changes from dark to light, and the sample image is obtained by using the self-made APP on the smartphone, which has a built-in image processing program , which can convert the corresponding image information into grayscale values. According to several groups of gray values ​​and corresponding pesticide concentration values, the relationship curve is fitted by regression analysis. Thus, the linear relationship between the gray value and the concentration of 2,4-D is obtained, thereby realizing the on-site handheld detection of 2,4-D (such as figur...

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Abstract

The invention discloses a 2, 4-dichlorophenoxyacetic acid (2, 4-D) on-site quantitative detection platform based on the integration of a target response type 3D printing model and a smart phone, and belongs to the technical field of biosensors. Based on a prepared carbon dot (CDs) / CoOOH composite material, the alkaline phosphatase can catalyze a substrate L-ascorbic acid-2-trisodium phosphate to generate the ascorbic acid with high reducibility, so that CoOOH nanosheets are effectively reduced into cobalt ions to further decompose the CDs / CoOOH composite material. The 2, 4-D is introduced to regulate and control a fluorescence response signal of the CDs / CoOOH composite material so as to realize the quantitative detection of the CDs / CoOOH composite material, and on the basis, the collectionand analysis of the fluorescence image data are further realized in combination with a 3D printing model and a smartphone application program. The quantitative detection platform has the advantages of being low in background and low in cost, being convenient to carry and the like, the 2, 4-D can be detected on site, and a new method is provided for the portable monitoring of food safety and lifehealth.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to an on-site quantitative detection platform for 2,4-dichlorophenoxyacetic acid integrated with a target-responsive 3D printing model and a smart phone. Background technique [0002] 2,4-Dichlorophenoxyacetic acid (2,4-D) is a phenoxyacetic acid herbicide and also a plant growth regulator, widely used in agricultural production and environmental epidemic prevention. However, this substance is a medium-persistent chemical substance with a half-life of 20 to 312 days, and is considered a compound that may be carcinogenic to humans. The abuse and improper treatment of 2,4-D will cause organ damage and neurobehavioral developmental retardation to many aquatic and mammals, and the resulting environmental and life safety hazards have become the focus of public attention. At present, routine pesticide analysis methods in the laboratory, such as high performance liquid chro...

Claims

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Application Information

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IPC IPC(8): G01N21/64G06T7/90
CPCG01N21/6428G06T2207/10064G06T7/90
Inventor 卢革宇闫旭苏丹丹孙鹏刘方猛王晨光刘晓敏高原梁喜双刘凤敏
Owner JILIN UNIV
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