caco 3 /mgo nanocomposite and its application in bone repair
A nanocomposite, bone powder technology, applied in nanotechnology, nanotechnology, calcium carbonate/strontium/barium, etc., can solve problems such as biological toxicity, and achieve the effect of reducing biological toxicity, good bone regeneration ability, and improving biological activity.
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Embodiment 1
[0051] Example 1CaCO 3 Characterization of MgO / MgO nanocomposites
[0052] our CaCO 3 / MgO nanocomposites were subjected to X-ray diffraction (XRD) analysis to determine the crystal structure of the samples. Such as figure 1 As shown, the X-ray diffraction 2θ characteristic peaks are located at 23.0°, 29.5°, 31.4°, 35.8°, 39.4°, 43.16°, 47.2°, 47.5°, 57.3°, 60.8° and 64.6°, corresponding to the (012), (104), (006), (110), (11-3), (202), (024), (018), (122), (208) and (300) planes (CaCO 3 , JCPDS No. 47-1743). The characteristic diffraction peaks are located at 57.5° and 63.3° corresponding to the (101) crystal plane and (103) crystal plane of periclase, respectively (MgO, JCPDS No. 87-0653). In addition, no other diffraction peaks were observed from the XRD pattern, indicating that CaCO 3 / MgO nanocomposites with only MgO and CaCO 3 Crystal structure. The results showed that the MgO nanoparticles prepared by dipping and roasting method combined well on the eggshell sur...
Embodiment 2
[0054] Example 2 is based on CaCO 3 / MgO-prepared scaffolds have good biocompatibility and mechanical properties
[0055] Preparation of CaCO by chemical cross-linking 3 / MgO / CMC composite scaffold: the CaCO 3 / MgO nanocomposites and CMC dissolved in ddH 2 O, use ultrasonic dispersion to form a uniform mixed solution, then use EDC and NHS to crosslink the mixed solution, and solidify within 30 minutes to form a hydrogel, and finally prepare a composite scaffold by freeze-drying the hydrogel (see Figure 4 ). The composite scaffold has an interconnected porous structure with a pore size between 50 μm and 80 μm. After co-cultured with cells for 3 days, many flower-like microspheres with a diameter of nearly 10 μm and a pore diameter of nearly 1 μm were produced on the surface of the scaffold, suggesting mineralization of the scaffold. After further EDS analysis of the composite scaffold, in addition to the basic elements such as carbon, oxygen, sodium, magnesium and calcium,...
Embodiment 3
[0058] Example 3CaCO 3 / MgO / CMC Scaffold Promotes Bone Repair
[0059] We performed ALP and ARS staining on the hADSCs cultured on the scaffolds to further prove the osteogenic inductivity of the scaffolds. When hADSCs were cultured on scaffolds for 7 days, CaCO 3 / MgO / CMC group enhanced ALP activity ( Figure 7 A). Meanwhile, when the cells were cultured on the scaffold for 21 days, the CaCO 3 / MgO / CMC group showed obvious calcium mineral nodules, while CMC group had almost no nodules ( Figure 7 B). These results indicated that the CaCO3 / MgO / CMC scaffold had better osteogenic differentiation potential.
[0060] Evaluation of CaCO2 by Standard Defect Repair Experiment in Rat Skull 3 Osteogenic inductivity of / MgO / CMC scaffolds. hADSCs were seeded on scaffolds, and after 3 days of in vitro culture, the cell / scaffold complexes were transplanted into rat bone defects. After 8 weeks of implantation, the rat skull samples were removed, made into paraffin sections after de...
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