Lactobacillus reuteri and application thereof

A technology of Lactobacillus reuteri and bacteria agent, applied in the field of microorganisms, can solve the problems of dependence on imports of bacteria, huge differences in application effects, instability, etc., and achieve the effects of excellent effect, enhanced immune function of the body, and good growth performance

Active Publication Date: 2020-08-14
SHENYANG BOYANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the probiotics widely used in canine animals, including pet dogs, are mainly non-canine probiotics. The strains mainly rely on imports, and the application effects vary greatly and are unstable.

Method used

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  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Isolation and identification of canine source Lactobacillus reuteri

[0050] (I) medium for separation: MRS medium, the composition of the medium is as follows:

[0051] Element weight Element weight Peptone 10.0g K 2 HPO 4

2.0g Beef Extract 10.0g NaAc 2.0g Yeast extract 5.0g MnSO 4 4H 2 o

0.25g glucose 20.0g MgSO 4 ·7H 2 o

0.58g Tween 80 1.0ml agar 20.0g Diammonium citrate 2.0g distilled water 1000mL

[0052] pH 6.2-6.5, sterilized at 121°C for 30 minutes.

[0053] (II) Separation method:

[0054] (1) Sample collection and microbial isolation: select healthy adult blue foxes from the fur animal breeding base of the Institute of Specialty Products, Chinese Academy of Agricultural Sciences, collect the contents of the hindgut, and quickly put them into a sterilized screw-top test tube with a liquid medium After being brought back to the laboratory, serial dilution was...

Embodiment 2

[0066] Embodiment 2 Physiological and biochemical characteristics

[0067] The biolog microbial identification instrument was used to detect the physiological and biochemical characteristics and enzyme activity of the strains, and the results are shown in Table 1 and Table 2.

[0068] Table 1 Physiological and biochemical characteristics of strain ZJF036—acid production using carbon source

[0069]

[0070]

[0071] +: positive reaction; -: negative reaction.

[0072] Table 2 Physiological and biochemical characteristics of strain ZJF036-enzyme activity

[0073]

[0074]

[0075] +: Positive reaction; -: Negative reaction; W: Weak positive reaction.

Embodiment 3

[0076] Embodiment 3 growth characteristics and acid production performance measurement

[0077] Take the bacterial liquid in the logarithmic growth phase, inoculate it into the MRS culture medium according to the inoculum amount of 2.0% (V / V), take the uninoculated MRS culture medium as the blank, culture it at 37°C for 24 hours, and take the culture medium every 2 hours, respectively. Using a microplate reader and a pH meter to measure the absorbance value (OD) at 600 nm 600 ) and pH value, taking time as the abscissa, the OD of the culture solution 600 and pH as the ordinate, draw the growth curve and acid production curve, the results are as follows Figure 4 shown.

[0078] The growth curve of ZJF036 within 24 hours was divided into slow growth period, rapid growth period, stable period, and did not enter the decline period, which was in line with the general growth law of bacteria. by OD 600 The value found that the OD of the bacterial solution within 0-4h after inocu...

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Abstract

The invention relates to the technical field of microorganisms, and particularly provides Lactobacillus reuteri and application thereof. The Lactobacillus reuteri provided by the invention is a canine-derived Lactobacillus reuteri strain which is separated from hindgut content of a healthy adult blue fox for the first time by an inventor, and the preservation number of the Lactobacillus reuteri isCCTCC NO: M 2018943. The strain has good growth performance, is high in tolerance to gastric juice and intestinal juice and good in adhesion to small intestine epithelial cells, can promote proliferation of dog intestinal tract beneficial bacteria, can inhibit growth of harmful bacteria, can enhance the immune function of pet dog organisms, has better effect than that of similar products in the market, can serve as an alternative strain of probiotic preparations, and is quite wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus reuteri and application thereof. Background technique [0002] Probiotics can beneficially affect the health of host animals by improving the intestinal microecological balance of host animals. Many beneficial probiotic strains have been successfully developed and verified, most of which are derived from human intestines, dairy products or plant surfaces, and have been used in food or It is widely used in feed. In 2013, the Ministry of Agriculture of my country approved that Lactobacillus reuteri can be used as a feed additive for animal breeding as a probiotic. Lactobacillus reuteri (Lactobacillus reuteri) widely exists in human and animal intestines, can inhibit the reproduction of gastrointestinal pathogens, regulate animal immune function, reduce animal stress, reduce animal morbidity, regulate intestinal flora, prevent or alleviate Diarrhea, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10C12R1/225
CPCC12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/3204
Inventor 刘晗璐张如春司华哲李光玉
Owner SHENYANG BOYANG FEED
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