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Preparation method of monatomic biomimetic enzyme, and product and application thereof

A biomimetic enzyme and atom technology, applied in the field of materials, can solve problems such as disadvantages, and achieve the effects of improving electrical conductivity, convenient material acquisition, and improving electron transfer performance.

Active Publication Date: 2020-08-25
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complex structure and atomic composition of nanomaterials are not conducive to the identification of intrinsic active sites for biocatalysis

Method used

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  • Preparation method of monatomic biomimetic enzyme, and product and application thereof
  • Preparation method of monatomic biomimetic enzyme, and product and application thereof
  • Preparation method of monatomic biomimetic enzyme, and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In the synthesis of superoxide dismutase biomimetic enzyme material (A-Co-NSG), the specific steps are as follows:

[0037] (1) Preparation of carbon nanomaterials with different defect structures: the melamine (C 3 h 6 N 6 ) (12g), 1-cysteine ​​(C 3 h 7 NO 2 S) (3g) and cobalt acetate tetrahydrate (Co(CH 3 COO) 2 4H 2 O) (0.2 g) was ground into precursors; subsequently, a two-stage pyrolysis and carbonization process was carried out (first stage: from 25 to 600 °C, at a heating rate of 3 °C / min, at 600 °C for 2 h ; the second stage: 600 ° C to 900 ° C, with a heating rate of 2 ° C / min, in a tube furnace under an argon atmosphere at 900 ° C for 1 h); after cooling to room temperature, the obtained monoatomic metal The graphene-like material of Co was leached in 1mol / L HCl at 80°C for 12 hours, and then in 1mol / L HNO 3 leaching for 24 hours to remove metal particles and unstable substances; after that, the sample was heated again in argon at 850°C for 1 hour t...

Embodiment 2

[0047] Superoxide dismutase biomimetic enzyme material, the specific steps are as follows:

[0048] Graphene oxide (GO) and melamine (C 3 h 6 N 6 ) and 3g / L cysteine ​​(C 3 h 7 NO 2 S) were ground together into a homogeneous mixture, and pyrolyzed in an argon atmosphere at 1000 °C for 2 hours; after cooling to room temperature, GO was dispersed in deionized water by ultrasound, and cobalt acetate tetrahydrate (Co(CH 3 COO) 2 4H 2 O) The mixture was stirred in a water bath at 80°C for 2 hours, and then freeze-dried for 24 hours to remove deionized water (DIW). Subsequently, with 500mg C 3 h 6 N 6 The dried product was ground into a homogeneous mixture and then pyrolyzed at 900 °C for 2 h in argon. Obtain biomimetic enzyme material (abbreviated as Co-NSG biomimetic enzyme material) by cooling to room temperature;

[0049] Working electrode coated with Co-NSG biomimetic enzyme material:

[0050] The prepared Co-NSG biomimetic enzyme material was dispersed in water at...

Embodiment 3

[0056] Superoxide dismutase biomimetic enzyme material, the specific steps are as follows:

[0057] Disperse graphene in deionized water, then add potassium dihydrogen phosphate under continuous stirring, then add cobalt sulfate, stir and react at 30°C for 3 hours, centrifuge at 5000r / min for 10min, take the precipitate, and wash the precipitate with deionized water After washing, vacuum-dry at 80° C. for 12 hours to prepare superoxide dismutase biomimetic enzyme material; wherein, the mass ratio of graphene, potassium dihydrogen phosphate and cobalt sulfate is 20:5:10.

[0058] Working electrodes coated with single-atom biomimetic enzyme materials:

[0059] Disperse the prepared superoxide dismutase biomimetic material in water at a ratio of 0.5 mg / mL to obtain an electrode modification solution, apply the electrode modification solution to a glassy carbon electrode, dry at 20°C for 10 h, and then apply Cover with a Nafion solution with a mass fraction of 0.1% of Nafion, and...

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Abstract

The invention discloses a preparation method of a monatomic biomimetic enzyme as well as a product and application thereof. The specific method comprises the following steps: physically compounding asix-membered heterocyclic compound, biomass amino acid and acetate containing a transition metal source; then, under the protection of inert gas, performing variable-speed heating for pyrolysis; further carrying out annealing reaction treatment in an inert atmosphere; then, using strong acid treatment for removing metal particles and unstable substances; and finally, heating to recover the crystallinity of carbon in an inert atmosphere, modifying a working electrode of an electrochemical sensor with the prepared monatomic biomimetic enzyme, and applying the monatomic biomimetic enzyme to detection of superoxide anions released by cells, wherein the monatomic biomimetic enzyme has excellent selectivity, extremely short response time, relatively low detection limit and extremely high reaction sensitivity. Compared with an electrochemical sensor prepared on the basis of a traditional material, a sensor constructed on the basis of the monatomic biomimetic enzyme shows higher performance and has an important application prospect in the aspect of in-situ real-time detection of superoxide anion free radicals released by living cells.

Description

technical field [0001] The invention relates to the technical field of materials, in particular to a preparation method of a single-atom biomimetic enzyme, and also relates to a sensor prepared by the single-atom biomimetic enzyme and the application of the sensor. Background technique [0002] Superoxide anion (O 2 .- ) is the product of the reduction of oxygen molecules by a single electron, and is also the first free radical formed by cells in the process of oxygen metabolism. All other reactive oxygen species (ROS) are derived from O 2 .- Derived from. o 2 .- Concentration fluctuations are closely related to the occurrence and development of many biological processes and diseases. Under normal physiological conditions, O 2 .- The concentration in cells will be controlled in a low range and maintain a relatively stable dynamic balance, which can assist cells in normal growth and metabolism, and has unique physiological effects. Its physiological functions mainly ...

Claims

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Application Information

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IPC IPC(8): B01J23/75G01N27/327
CPCB01J23/75G01N27/3272B01J35/33
Inventor 李长明邹卓杨洪斌吴超
Owner SOUTHWEST UNIVERSITY
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