Lactobacillus casei for prevention and auxiliary treatment of type II diabetes and application of lactobacillus casei

A technology of Lactobacillus casei and adjuvant therapy, which is applied in the field of microbiology and can solve problems such as drug dependence and drug resistance

Inactive Publication Date: 2020-08-25
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, diabetes is often treated with drugs, which can easily lead to drug dependence and drug resistance

Method used

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  • Lactobacillus casei for prevention and auxiliary treatment of type II diabetes and application of lactobacillus casei
  • Lactobacillus casei for prevention and auxiliary treatment of type II diabetes and application of lactobacillus casei
  • Lactobacillus casei for prevention and auxiliary treatment of type II diabetes and application of lactobacillus casei

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Experimental program
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Effect test

preparation example Construction

[0033] The application provides a method for preparing probiotic compressed candy, the preparation method comprising: mixing and sieving the raw materials, and then compressing the tablets to obtain probiotic compressed candy, wherein the raw materials include bacteria powder of Lactobacillus casei, Skim milk powder, fruit powder, galacto-oligosaccharide powder, xylitol, microcrystalline cellulose and magnesium stearate.

[0034] In an achievable manner, the raw material mass ratio of the probiotic compressed candy is: 4-5 parts of bacteria powder, 20-30 parts of skim milk powder, 20-30 parts of fruit powder, 10 parts of galacto-oligosaccharide powder -15 parts, 4-5 parts of xylitol, 5-10 parts of microcrystalline cellulose, 0.5-1 part of magnesium stearate.

[0035] Put the bacteria powder of Lactobacillus casei according to the above mass ratio in the probiotic tablet candy, the number of viable probiotic bacteria is 10 9 CFU / g, the moisture content is less than 4%, and the...

Embodiment 1

[0040] Example 1: Isolation, identification and in vitro functional evaluation of Lactobacillus casei LCZ.

[0041] 1. Isolation and identification of Lactobacillus casei LCZ

[0042]Lactobacillus casei was isolated from the traditional kumiss in Xilin Gol Prairie, Inner Mongolia, and passed through MRS medium (Difco TM Lactobacilli MRS Agar), cultivated at 37°C for 72 hours, isolated a single colony, centrifuged to extract bacterial DNA, and then performed bacterial 16S universal primer PCR, and was identified as Lactobacillus casei by comparison of 16S rDNA.

[0043] For the Lactobacillus casei strain of the present application, a single colony is inoculated on the MRS solid medium, and it grows well aerobically at 37 ° C. The colony is round, with a diameter of 2.0-3.0 mm, the surface is not smooth, and it is milky white; Gram staining is positive , the thallus is relatively straight or slightly curved, with blunt ends, and exists in single or double.

[0044] 2. Evalua...

Embodiment 2

[0047] Example 2: Intervention of Lactobacillus casei LCZ for 2 weeks can well prevent the progress of type Ⅱ diabetes.

[0048] 8-week-old male SD rats were selected and divided into normal control group, prevention group, model group and treatment group. Except for the normal group, all other groups were fed with high-fat diet for 2 weeks, and then treated with STZ (streptozocin, streptozocin). ) induction to establish a type Ⅱ diabetes rat model. Among them, the prevention group self-administered Lactobacillus casei LCZ powder 4×10 9 cfu / g for 2 weeks. After modeling, the prevention group and the treatment group were intragastrically administered 5.0×10 9 cfu / g, each group was fed with normal feed for 8 weeks. Such as image 3 and 4 As shown, the fasting and postprandial blood glucose level indicators showed that the intake of Lactobacillus casei LCZ 2 weeks in advance had significant preventive effects on the fasting and postprandial blood glucose levels and prevent...

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Abstract

The invention discloses lactobacillus casei for prevention and auxiliary treatment of type II diabetes and application of the lactobacillus casei. The lactobacillus casei LCZ has a remarkable functionof preventing type II diabetes and the process of the type II diabetes can be well prevented after the lactobacillus casei intervenes for two weeks; the weight increase of a high-fat diet mice can beintervened and delayed, and the oral glucose tolerance increase induced by high-fat diet is prevented; expression of intestinal tract iron-related protein (transferrin and iron transport carrier protein) can be improved, chronic inflammation caused by endotoxin entering tissue is reduced, and then the effect of lowering the hyperglycemia level is achieved. Due to the remarkable functional characteristics of the lactobacillus casei, the lactobacillus casei has wide application value in various forms of probiotic foods, such as probiotic tabletting candies and probiotic solid beverages.

Description

technical field [0001] The application relates to the technical field of microbes, in particular to a strain of Lactobacillus casei for the prevention and auxiliary treatment of type II diabetes and its application. Background technique [0002] Diabetes is a common chronic metabolic disease often accompanied by life-threatening complications such as heart disease, atherosclerosis, nephropathy, neuropathy, and retinopathy. Type Ⅱ diabetes accounts for about 95% of diabetic patients. Type Ⅱ diabetes is a common chronic metabolic disease, and its pathogenesis is very complex. Traditionally, diabetes has been treated with drugs, which can easily lead to drug dependence and drug resistance. Contents of the invention [0003] The purpose of this application is to provide a strain of Lactobacillus casei LCZ (Lactobacillus casei zhang) for the prevention and auxiliary treatment of type II diabetes. [0004] The purpose of this application is achieved through the following tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/10A23L33/135A23G3/36A23G3/38A23G3/46A23G3/48A23L2/39A23L2/52A23L2/60A23L2/68C12R1/245
CPCA61K35/747A61P3/10A23L33/135A23G3/366A23G3/46A23G3/48A23G3/36A23G3/38A23L2/39A23L2/52A23L2/60A23L2/68A23V2002/00C12R2001/245C12N1/205A23V2400/125A23V2200/328
Inventor 刘晓军马杰张凌宇张建军其木格苏都杨慧娟
Owner BEIJING SCITOP BIO TECH CO LTD
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